##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474008
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946467148233786	31.0	31.0	33.0	28.0	34.0
2	31.29106681743768	31.0	31.0	34.0	28.0	34.0
3	31.36103610065653	31.0	31.0	34.0	28.0	34.0
4	35.24681861909504	35.0	35.0	37.0	33.0	37.0
5	34.952806703684324	35.0	35.0	37.0	32.0	37.0
6	35.04293809387183	36.0	35.0	37.0	32.0	37.0
7	34.867546539298914	35.0	35.0	37.0	32.0	37.0
8	34.927395740156285	36.0	35.0	37.0	32.0	37.0
9	36.41074834180014	38.0	35.0	39.0	32.0	39.0
10	36.19225835850872	38.0	35.0	39.0	32.0	39.0
11	36.324697051526556	38.0	35.0	39.0	32.0	39.0
12	36.05927115154174	38.0	35.0	39.0	31.0	39.0
13	36.14470641845707	38.0	35.0	39.0	31.0	39.0
14	36.927606707059795	39.0	36.0	40.0	31.0	41.0
15	37.09148790737709	39.0	36.0	40.0	31.0	41.0
16	37.079935359740766	39.0	36.0	40.0	31.0	41.0
17	37.15303117246966	39.0	36.0	40.0	31.0	41.0
18	37.15986017113635	39.0	36.0	40.0	31.0	41.0
19	37.26026353985587	39.0	36.0	40.0	31.0	41.0
20	37.25292611095171	39.0	36.0	40.0	31.0	41.0
21	37.169982363166866	39.0	36.0	40.0	31.0	41.0
22	37.08948161212469	39.0	36.0	40.0	31.0	41.0
23	36.972152368736396	39.0	36.0	40.0	31.0	41.0
24	36.88678883056826	39.0	35.0	40.0	31.0	41.0
25	36.787811598116484	38.0	35.0	40.0	30.0	41.0
26	36.56951992371437	38.0	35.0	40.0	30.0	41.0
27	36.43993983223912	38.0	35.0	40.0	30.0	41.0
28	36.27230764037738	38.0	35.0	40.0	30.0	41.0
29	36.075445984034026	38.0	35.0	40.0	30.0	41.0
30	35.84430642520801	38.0	34.0	40.0	29.0	41.0
31	35.650588175726995	38.0	34.0	40.0	28.0	41.0
32	35.519721186140316	38.0	34.0	40.0	28.0	41.0
33	35.35314804813421	38.0	34.0	40.0	27.0	41.0
34	35.50231008759346	38.0	34.0	40.0	27.0	41.0
35	35.427429494860846	38.0	34.0	40.0	27.0	41.0
36	35.38559264822535	38.0	34.0	40.0	27.0	41.0
37	35.28056488498085	38.0	34.0	40.0	27.0	41.0
38	35.261634824728695	38.0	34.0	40.0	26.0	41.0
39	35.179323977654384	38.0	34.0	40.0	25.0	41.0
40	34.99786712460549	38.0	33.0	40.0	25.0	41.0
41	34.89055669946499	38.0	33.0	40.0	24.0	41.0
42	34.744571821572634	38.0	33.0	40.0	24.0	41.0
43	34.3782974127019	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	6.0
17	8.0
18	29.0
19	68.0
20	146.0
21	339.0
22	633.0
23	1052.0
24	1744.0
25	2840.0
26	4259.0
27	6277.0
28	9033.0
29	12268.0
30	15505.0
31	19724.0
32	24131.0
33	29318.0
34	35364.0
35	42471.0
36	52032.0
37	65518.0
38	78823.0
39	72419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83202815142361	22.625567500970448	14.580344635533576	22.962059712072367
2	18.06045467587045	23.910566910263118	36.288206106226056	21.740772307640377
3	19.249675110968592	25.45146917351606	33.39901436262679	21.899841352888558
4	13.341336011206561	16.57904507940794	37.306332382575825	32.77328652680968
5	11.97574724477224	38.517493375639226	36.042218696730856	13.464540682857674
6	31.01972962481646	38.09408280029029	14.130141263438592	16.756046311454657
7	24.41477780965722	34.225160756780475	22.43211085044978	18.927950583112523
8	25.6930262780375	35.14307775396196	20.034682958937402	19.129213009063136
9	23.886094749455705	14.915360078310917	23.02978008809978	38.1687650841336
10	15.689608614200603	27.719785320078987	34.97493713186275	21.615668933857656
11	31.96169684899833	22.791809420938044	24.774898313952505	20.47159541611112
12	20.525391976506725	27.285193498843903	32.02266628411335	20.166748240536027
13	30.767413208215892	21.765455435351303	27.503966177786026	19.963165178646776
14	19.80662773624074	23.703608377917675	28.857529830720157	27.632234055121437
15	21.590141938532685	30.20539737725946	26.580142107306205	21.624318576901654
16	21.07622656157702	29.387056758535724	27.48202561982076	22.054691060066496
17	20.66041079475452	27.39721692460887	27.846154495282782	24.096217785353836
18	22.322197093719936	26.121078125263708	30.83281294830467	20.723911832711682
19	21.70722856998194	25.408853859006598	32.10852981384281	20.775387757168655
20	22.961848745168858	24.771733810399823	32.068235135271976	20.19818230915934
21	21.797733371588667	25.74028286442423	32.39017063003156	20.071813133955548
22	20.255987240721677	25.994919916963426	31.904313851243018	21.84477899107188
23	21.408077500801674	26.224029974177647	32.801767058783824	19.566125466236855
24	21.262088403571248	26.189220435098143	30.6410440330121	21.90764712831851
25	20.984666925452736	26.659254696123273	31.597357006632798	20.758721371791193
26	20.86884609542455	27.600378052691095	31.260653828627365	20.27012202325699
27	19.58701118968456	27.497004269970127	31.080277126124457	21.835707414220856
28	20.32602825268772	26.89448279353935	31.174157398187376	21.60533155558556
29	20.588893014463892	26.561787986700647	30.43450743447368	22.414811564361784
30	20.597331690604378	26.985831462760125	32.30114259674942	20.11569424988608
31	21.801741742755397	26.535628090665135	31.915073163322138	19.74755700325733
32	21.026438372348146	26.610521341411957	30.782180891461746	21.580859394778148
33	20.668427537087982	26.509257227726113	31.528792762991344	21.293522472194564
34	19.760847918178595	27.297218612344093	32.33278763227625	20.60914583720106
35	19.356846297952774	29.352247219456213	30.824585239067698	20.466321243523318
36	21.731067830078818	27.13329732831513	30.52543416988743	20.61020067171862
37	20.85323454456465	27.89066851192385	30.487249160351727	20.768847783159778
38	20.646697946026226	26.30651803345091	31.794400094513176	21.25238392600969
39	20.242907292703922	25.86137786704022	32.571602167052035	21.324112673203828
40	18.89060944119087	26.336686300653156	32.2937587551265	22.478945503029486
41	20.60893487029755	25.60062277429917	32.0530455182191	21.737396837184182
42	19.05474169212334	27.79868694199254	31.50242190005232	21.6441494658318
43	19.613171085720072	27.796999206764443	30.608766096774737	21.981063610740748
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	50.0
2	67.0
3	201.0
4	335.0
5	335.0
6	542.0
7	749.0
8	832.5
9	916.0
10	1386.0
11	1856.0
12	1856.0
13	3410.0
14	4964.0
15	8285.0
16	11606.0
17	11302.0
18	10998.0
19	10998.0
20	11803.0
21	12608.0
22	10770.0
23	8932.0
24	9838.5
25	10745.0
26	10745.0
27	11879.5
28	13014.0
29	13936.0
30	14858.0
31	16133.5
32	17409.0
33	17409.0
34	18917.5
35	20426.0
36	21987.5
37	23549.0
38	24533.5
39	25518.0
40	25518.0
41	26158.5
42	26799.0
43	27563.0
44	28327.0
45	30042.5
46	31758.0
47	31758.0
48	41770.0
49	51782.0
50	46964.0
51	42146.0
52	37538.5
53	32931.0
54	32931.0
55	28752.5
56	24574.0
57	20256.5
58	15939.0
59	14081.0
60	12223.0
61	12223.0
62	10768.0
63	9313.0
64	7985.0
65	6657.0
66	5645.0
67	4633.0
68	4633.0
69	3877.5
70	3122.0
71	2650.5
72	2179.0
73	1751.5
74	1324.0
75	1324.0
76	1061.5
77	799.0
78	601.5
79	404.0
80	333.5
81	263.0
82	263.0
83	192.0
84	121.0
85	95.5
86	70.0
87	51.5
88	33.0
89	33.0
90	26.0
91	19.0
92	13.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	474008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.149709262495534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.33954438254644	54.52317125614135
2	8.360260849319038	10.558960839862426
3	2.210643628938876	4.188045073514343
4	0.923176865105472	2.3319340251709044
5	0.4761640127573847	1.5034809483446028
6	0.3175840894339627	1.2033205748489475
7	0.2091669768202021	0.9246183641457634
8	0.15295517018657634	0.7727259621982255
9	0.13002175551655842	0.7389752452802945
>10	0.7857464846687852	9.260704068594054
>50	0.054565134018113456	2.39153512624299
>100	0.03314078681806374	4.664265084621229
>500	0.003682309646451527	1.577444981029895
>1k	0.002678043379237474	3.1280425334091873
>5k	6.695108448093685E-4	2.2327759165957852
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5319	1.1221329597812695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5243	1.106099475114344	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4435	0.935638217076505	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.37109078327791933	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1506	0.3177161566893386	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1451	0.30611297699616885	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1449	0.3056910431891445	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1440	0.3037923410575349	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1396	0.2945097973029991	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1361	0.2871259556800729	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	988	0.20843530067003088	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	776	0.16371031712544937	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	767	0.16181161499383975	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	757	0.159701945958718	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.15252907123930398	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	674	0.14219169296720732	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	632	0.13333108301969587	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	553	0.11666469764223389	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	544	0.11476599551062429	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	540	0.11392212789657558	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	508	0.10717118698418593	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	484	0.10210798129989367	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	482	0.10168604749286933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.10966903512177E-4	0.0
12	0.0	0.0	0.0	0.0014767683245852392	0.0
13	0.0	0.0	0.0	0.0018987021316095932	0.0
14	0.0	0.0	0.0	0.0018987021316095932	0.0
15	0.0	0.0	0.0	0.002320635938633947	0.0
16	0.0	0.0	0.0	0.002742569745658301	0.0
17	0.0	0.0	0.0	0.004219338070243541	0.0
18	0.0	0.0	0.0	0.005063205684292248	0.0
19	0.0	0.0	0.0	0.006118040201853133	0.0
20	0.0	0.0	0.0	0.009493510658047965	0.0
21	0.0	0.0	0.0	0.017932186798535046	0.0
22	0.0	0.0	0.0	0.025737962228485595	0.0
23	0.0	0.0	0.0	0.03291083694789961	0.0
24	0.0	0.0	0.0	0.04177144689541105	0.0
25	0.0	0.0	0.0	0.05021012303589813	0.0
26	0.0	0.0	0.0	0.06413393866770181	0.0
27	0.0	0.0	0.0	0.15527164098496227	0.0
28	0.0	0.0	0.0	0.25801252299539246	0.0
29	0.0	0.0	0.0	0.37657592276923596	0.0
30	0.0	0.0	0.0	0.5807918853690233	0.0
31	0.0	0.0	0.0	0.8434456802416836	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	35	2.3853698E-5	31.714285	5
GGTATCA	1405	0.0	28.967972	1
CGGGTCT	40	5.932878E-5	27.750002	6
ACCGTCG	35	8.8616763E-4	26.42857	23
GTGGCGT	40	0.001929522	23.125002	6
CCGTCGG	40	0.001929522	23.125002	24
TCACGTA	40	0.001929522	23.125002	25
CAGACCG	65	2.6773268E-6	22.76923	20
GGCGTGA	50	2.698858E-4	22.199999	8
GACCGTC	45	0.0038225416	20.555555	22
TACGGGT	90	9.4487405E-8	20.555555	4
TACCTGC	45	0.0038225416	20.555555	6
CTAGCCG	45	0.0038225416	20.555555	10
TACTCCG	55	5.13697E-4	20.181818	5
CTCTAGG	70	1.2172804E-4	18.5	1
CTAATAC	80	1.6134063E-5	18.5	3
CTCCTAA	50	0.0070289345	18.499998	1
GCTCGAG	50	0.0070289345	18.499998	29
GCGTCGG	50	0.0070289345	18.499998	9
TAGCTCG	50	0.0070289345	18.499998	27
>>END_MODULE