FastQCFastQC Report
Wed 25 May 2016
SRR2088311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502402
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.9542159465925694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47320.94187523138841No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39600.7882134227172664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.3521084709057687No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG10670.21237972778770783No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA9920.1974514432665475No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9780.1946648301559309No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG9370.1865040346176966No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA8980.1787413266666932No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7300.14530196933929404No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA6740.13415551689682764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.12898037826282538No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC5550.11046930545658656No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.10708556096512355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT303.5966208E-430.8333324
ACGCTAT250.00549343529.632
GGTATCA11500.027.5086961
CTATGCA450.003822856320.5555554
GGATAGG450.003822856320.5555551
ATTGGAC909.451651E-820.5555553
GTGACCC555.137557E-420.18181815
TACCGAC555.137557E-420.1818187
CTAATAC951.671433E-719.4736843
GTATTGG1409.276846E-1118.51
TAGGGTG500.00702950518.55
GTATAGA500.00702950518.51
TAGGCTA500.00702950518.54
TGTACTG1306.9303496E-1018.55
TACTGGT1107.7861114E-716.8181827
GTATCAA19200.016.3802072
TTGGACC1708.367351E-1116.323534
AATACTG1251.6521517E-716.2799995
GTCCAGT700.002589770415.8571431
TGATCAA855.356689E-415.23529410
Produced by FastQC (version 0.11.5)