##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	502402
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.963768058248174	31.0	31.0	33.0	28.0	34.0
2	31.3013124947751	31.0	31.0	34.0	28.0	34.0
3	31.388380221416316	31.0	31.0	34.0	28.0	34.0
4	35.2757015298506	37.0	35.0	37.0	33.0	37.0
5	35.008706175532744	35.0	35.0	37.0	32.0	37.0
6	35.05010330372889	36.0	35.0	37.0	32.0	37.0
7	34.877054629559595	36.0	35.0	37.0	32.0	37.0
8	34.92022125708098	36.0	35.0	37.0	32.0	37.0
9	36.43860892273518	38.0	35.0	39.0	32.0	39.0
10	36.21114167539142	38.0	35.0	39.0	32.0	39.0
11	36.32384226177444	38.0	35.0	39.0	32.0	39.0
12	36.0744045604914	38.0	35.0	39.0	30.0	39.0
13	36.16217889259995	38.0	35.0	39.0	31.0	39.0
14	37.015654794367855	39.0	36.0	40.0	31.0	41.0
15	37.155240225954515	39.0	36.0	40.0	31.0	41.0
16	37.13405997587589	39.0	36.0	40.0	31.0	41.0
17	37.17302279847612	39.0	36.0	40.0	31.0	41.0
18	37.17405782620292	39.0	36.0	40.0	31.0	41.0
19	37.26523779762023	39.0	36.0	40.0	31.0	41.0
20	37.24620523007472	39.0	36.0	40.0	31.0	41.0
21	37.176876286320514	39.0	36.0	40.0	31.0	41.0
22	37.09554898268717	39.0	36.0	40.0	31.0	41.0
23	36.98508166766852	39.0	36.0	40.0	31.0	41.0
24	36.90339011389286	39.0	35.0	40.0	31.0	41.0
25	36.77012830362936	39.0	35.0	40.0	30.0	41.0
26	36.53540989088419	38.0	35.0	40.0	30.0	41.0
27	36.41245257781617	38.0	35.0	40.0	30.0	41.0
28	36.264254521279774	38.0	35.0	40.0	30.0	41.0
29	36.05699619030179	38.0	35.0	40.0	29.0	41.0
30	35.86277522780562	38.0	34.0	40.0	29.0	41.0
31	35.67102240835028	38.0	34.0	40.0	28.0	41.0
32	35.53319254302332	38.0	34.0	40.0	27.0	41.0
33	35.363290751231084	38.0	34.0	40.0	27.0	41.0
34	35.539026516614186	38.0	34.0	40.0	27.0	41.0
35	35.499325241539644	38.0	34.0	40.0	27.0	41.0
36	35.443527294875416	38.0	34.0	40.0	27.0	41.0
37	35.3557987428394	38.0	34.0	40.0	27.0	41.0
38	35.33205082782314	38.0	34.0	40.0	26.0	41.0
39	35.24105596713389	38.0	34.0	40.0	26.0	41.0
40	35.07025847827039	38.0	34.0	40.0	25.0	41.0
41	34.97381379851195	38.0	33.0	40.0	24.0	41.0
42	34.863684857942445	38.0	33.0	40.0	24.0	41.0
43	34.47922978013622	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	6.0
18	22.0
19	88.0
20	184.0
21	355.0
22	665.0
23	1181.0
24	2018.0
25	3194.0
26	4823.0
27	6661.0
28	9643.0
29	12971.0
30	16574.0
31	20416.0
32	25072.0
33	30352.0
34	36755.0
35	44044.0
36	53723.0
37	67856.0
38	83855.0
39	81940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79542278892202	22.789917237590615	14.203964156193646	23.210695817293722
2	18.02003176738946	23.79011230050836	35.93974546279672	22.250110469305458
3	19.139255018889255	25.535129239135195	32.95627804029442	22.369337701681125
4	13.514874542696884	17.114780594026296	37.26358573413322	32.10675912914359
5	12.351861656601686	38.655498982886215	35.57629149565487	13.416347864857226
6	30.980768388660874	38.34240309552908	14.804678325325138	15.87215019048491
7	24.67744953244613	33.9530893587207	23.0277745709611	18.34168653787206
8	25.446554750976308	35.25682620690204	20.16970473843655	19.126914303685098
9	24.245524500300554	14.908579185592414	23.001699833997478	37.84419648010955
10	15.573186412474474	28.160118789336032	35.00244823866147	21.264246559528026
11	32.04824025382065	23.51961178498493	24.464074585690344	19.968073375504076
12	20.160349680136626	27.634643174191186	32.002261137495466	20.20274600817672
13	30.015405989625837	22.217666330946134	27.24093455041978	20.525993129008242
14	19.94637760199999	23.67665733814754	28.949924562402217	27.42704049745025
15	21.96726923857787	30.354974701533834	26.139028108964535	21.53872795092376
16	21.053061094502013	29.245703639714808	27.809403624985567	21.89183164079761
17	20.781764403804125	27.90355133936569	28.260038773730994	23.054645483099193
18	21.8376917289342	26.973817779387822	30.461463131118112	20.727027360559873
19	21.706521868941607	26.35558775641817	31.717031381244503	20.220858993395726
20	22.312212132913483	25.93222160739806	31.281523560813852	20.474042698874605
21	21.105210568429268	26.821151189684755	31.895573664117578	20.1780645777684
22	20.379894984494488	27.11354652250588	31.352383151340955	21.15417534165867
23	21.22364162563047	27.043881194740464	31.79844029283323	19.934036886795834
24	21.149995421992745	26.98058526837075	30.516598261949596	21.352821047686913
25	21.031763408585157	27.406538986707858	31.08685873065792	20.474838874049066
26	20.64044331033714	28.050843746641135	30.90035469604022	20.4083582469815
27	19.957723098236073	27.8484162085342	30.732361734228764	21.46149895900096
28	20.31480766398223	27.63703169971457	30.833077893798194	21.215082742505007
29	20.59167758090135	27.589261189246855	30.350993825661522	21.46806740419027
30	20.762855243410655	27.79089255217933	31.399755574221437	20.046496630188575
31	21.423879682007634	27.61434070724241	31.051428935394366	19.91035067535559
32	20.86715419126516	27.644396320078346	30.59502151663409	20.893427972022405
33	20.6360643468776	27.545869642238685	30.90274322156361	20.915322789320108
34	20.121138052794375	27.716450173367146	31.569738973969052	20.592672799869426
35	19.775996114665148	29.19773408545348	30.728181814562838	20.29808798531853
36	21.36118088701876	27.715454954399064	30.366519241563527	20.556844917018644
37	20.751111659587345	28.06039784873468	30.451510941437338	20.736979550240644
38	20.483795844761765	27.153355281228976	31.16886477362749	21.193984100381766
39	20.092674790307363	26.94216981620296	32.023757867205944	20.941397526283733
40	19.317797301762337	27.03173952332992	31.702700228104185	21.947762946803557
41	20.284553007352677	26.349815486403315	31.848798372617942	21.516833133626058
42	19.327550447649493	27.83448314298112	31.198323255082585	21.639643154286805
43	19.657366013670327	27.87628233964037	30.459074605594722	22.007277041094582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	64.0
2	85.0
3	224.5
4	364.0
5	364.0
6	589.0
7	814.0
8	900.0
9	986.0
10	1557.0
11	2128.0
12	2128.0
13	3716.5
14	5305.0
15	8375.5
16	11446.0
17	11262.5
18	11079.0
19	11079.0
20	11942.5
21	12806.0
22	11300.0
23	9794.0
24	11205.0
25	12616.0
26	12616.0
27	13814.0
28	15012.0
29	15946.5
30	16881.0
31	18493.5
32	20106.0
33	20106.0
34	21902.0
35	23698.0
36	25636.5
37	27575.0
38	28563.0
39	29551.0
40	29551.0
41	29813.0
42	30075.0
43	30827.0
44	31579.0
45	32687.0
46	33795.0
47	33795.0
48	40213.0
49	46631.0
50	43011.5
51	39392.0
52	35106.0
53	30820.0
54	30820.0
55	27298.5
56	23777.0
57	20668.0
58	17559.0
59	15862.0
60	14165.0
61	14165.0
62	12335.0
63	10505.0
64	9205.5
65	7906.0
66	6715.5
67	5525.0
68	5525.0
69	4652.5
70	3780.0
71	3221.5
72	2663.0
73	2233.0
74	1803.0
75	1803.0
76	1435.0
77	1067.0
78	791.0
79	515.0
80	394.5
81	274.0
82	274.0
83	209.0
84	144.0
85	117.0
86	90.0
87	56.5
88	23.0
89	23.0
90	20.0
91	17.0
92	11.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	502402.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.6360191963137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88385008584997	54.016703135201624
2	9.268914956803926	11.796737002403475
3	2.449116023496213	4.675559828556135
4	1.0353804222847982	2.635499537120112
5	0.5827998510963722	1.8543531255988757
6	0.3300727444519593	1.2602709301274895
7	0.2369569546585926	1.0555298120747962
8	0.16560014961193525	0.8430507439694026
9	0.13634028029920348	0.7808537424915811
>10	0.8126923538917493	9.65671349477923
>50	0.060718403150303114	2.661494555583732
>100	0.03286305260439068	4.028191766008141
>500	0.0031298145337514908	1.484206924118302
>1k	0.0015649072668757454	3.250835401967113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4794	0.9542159465925694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4732	0.94187523138841	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3960	0.7882134227172664	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1769	0.3521084709057687	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1067	0.21237972778770783	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	992	0.1974514432665475	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	978	0.1946648301559309	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	937	0.1865040346176966	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	898	0.1787413266666932	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	730	0.14530196933929404	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	674	0.13415551689682764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.12898037826282538	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	555	0.11046930545658656	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.10708556096512355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.9904379361547128E-4	0.0
10	0.0	0.0	0.0	1.9904379361547128E-4	0.0
11	1.9904379361547128E-4	0.0	0.0	1.9904379361547128E-4	0.0
12	1.9904379361547128E-4	0.0	0.0	3.9808758723094257E-4	0.0
13	1.9904379361547128E-4	0.0	0.0	3.9808758723094257E-4	0.0
14	1.9904379361547128E-4	0.0	0.0	5.971313808464139E-4	0.0
15	1.9904379361547128E-4	0.0	0.0	5.971313808464139E-4	0.0
16	1.9904379361547128E-4	0.0	0.0	5.971313808464139E-4	0.0
17	1.9904379361547128E-4	0.0	0.0	9.952189680773563E-4	0.0
18	1.9904379361547128E-4	0.0	0.0	0.0011942627616928277	0.0
19	1.9904379361547128E-4	0.0	0.0	0.0017913941425392415	0.0
20	1.9904379361547128E-4	0.0	0.0	0.0027866131106165978	0.0
21	1.9904379361547128E-4	0.0	0.0	0.004777051046771311	0.0
22	1.9904379361547128E-4	0.0	0.0	0.009156014506311679	0.0
23	1.9904379361547128E-4	0.0	0.0	0.011146452442466391	0.0
24	1.9904379361547128E-4	0.0	0.0	0.018112985219007886	0.0
25	1.9904379361547128E-4	0.0	0.0	0.02189481729770184	0.0
26	1.9904379361547128E-4	0.0	0.0	0.030254656629551634	0.0
27	1.9904379361547128E-4	0.0	0.0	0.08956970712696208	0.0
28	1.9904379361547128E-4	0.0	0.0	0.1753575821752302	0.0
29	1.9904379361547128E-4	0.0	0.0	0.2708986031106564	0.0
30	1.9904379361547128E-4	0.0	0.0	0.4309298131774953	0.0
31	1.9904379361547128E-4	0.0	0.0	0.6620196575650574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACT	30	3.5966208E-4	30.833332	4
ACGCTAT	25	0.005493435	29.6	32
GGTATCA	1150	0.0	27.508696	1
CTATGCA	45	0.0038228563	20.555555	4
GGATAGG	45	0.0038228563	20.555555	1
ATTGGAC	90	9.451651E-8	20.555555	3
GTGACCC	55	5.137557E-4	20.181818	15
TACCGAC	55	5.137557E-4	20.181818	7
CTAATAC	95	1.671433E-7	19.473684	3
GTATTGG	140	9.276846E-11	18.5	1
TAGGGTG	50	0.007029505	18.5	5
GTATAGA	50	0.007029505	18.5	1
TAGGCTA	50	0.007029505	18.5	4
TGTACTG	130	6.9303496E-10	18.5	5
TACTGGT	110	7.7861114E-7	16.818182	7
GTATCAA	1920	0.0	16.380207	2
TTGGACC	170	8.367351E-11	16.32353	4
AATACTG	125	1.6521517E-7	16.279999	5
GTCCAGT	70	0.0025897704	15.857143	1
TGATCAA	85	5.356689E-4	15.235294	10
>>END_MODULE