FastQCFastQC Report
Wed 25 May 2016
SRR2088310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552222
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213921.3781533826991241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213581.375962974368357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186951.204402463049744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72130.464688684994801No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63310.40786691594372454No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG62630.4034860992821903No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA59770.38506089979397273No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59380.382548372591034No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG56340.3629635451629986No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54820.3531711314489809No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39560.25486045166219784No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34650.22322837841494322No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32430.2089263004905226No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC31470.20274161814482725No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29830.1921761191375976No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.17710095591996505No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27240.17549036155910688No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG22550.1452756113494075No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22490.14488906870280155No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG22400.14430925473289258No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG22130.14256981282316575No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20130.12968505793630036No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20100.12949178661299737No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG19180.12356479936503928No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.1212455434854035No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17940.11557625133518272No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17250.11113101089921416No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT16970.10932714521505299No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA15930.10262707267388299No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG15540.10011454547094424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.031.3292331
GTCGTCA551.9032159E-523.54545424
CGCGATA609.238803E-418.514
TTGGACC7550.017.8874194
ATTGGAC3850.017.7792223
GTATCAA93800.017.7505342
ACGTTTA852.724112E-517.41176426
TACGTGC752.0678541E-417.2666668
GTATTGG3750.016.7733331
GGACCCT7900.016.6265816
CAAACGA1352.2208042E-816.44444523
CGAACTA803.3835217E-416.187524
CTGTGCG1059.347243E-615.8571429
TGGACCC8800.015.7670455
CTAATAC2350.015.7446813
TGCGCGA957.0622205E-515.57894812
ATAGCGG1455.3487383E-815.31034537
CGAGTAT750.00410526714.810
TAGGTCG750.00410526714.821
TAGGCTC1001.093756E-414.85
Produced by FastQC (version 0.11.5)