##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088310_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552222
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.926289538480965	31.0	31.0	33.0	28.0	34.0
2	31.24707355004632	31.0	31.0	34.0	28.0	34.0
3	31.30542216255149	31.0	31.0	34.0	28.0	34.0
4	35.19816559744676	35.0	35.0	37.0	33.0	37.0
5	34.96264194168102	35.0	35.0	37.0	32.0	37.0
6	35.014250538904875	36.0	35.0	37.0	32.0	37.0
7	34.82384929475294	35.0	35.0	37.0	32.0	37.0
8	34.89401000630065	36.0	35.0	37.0	32.0	37.0
9	36.35548201223794	38.0	35.0	39.0	32.0	39.0
10	36.16342894250951	38.0	35.0	39.0	31.0	39.0
11	36.269251434395336	38.0	35.0	39.0	32.0	39.0
12	36.02376335343784	38.0	35.0	39.0	30.0	39.0
13	36.09631483125481	38.0	35.0	39.0	31.0	39.0
14	36.88767135113405	39.0	36.0	40.0	31.0	41.0
15	37.02089713971326	38.0	36.0	40.0	31.0	41.0
16	36.980311450295126	38.0	36.0	40.0	31.0	41.0
17	37.08168676903175	39.0	36.0	40.0	31.0	41.0
18	37.098650837315795	39.0	36.0	40.0	31.0	41.0
19	37.20297096678181	39.0	36.0	40.0	31.0	41.0
20	37.19228757226737	39.0	36.0	40.0	31.0	41.0
21	37.110678756002685	39.0	36.0	40.0	31.0	41.0
22	37.03821682723219	39.0	36.0	40.0	31.0	41.0
23	36.913353888812296	39.0	35.0	40.0	31.0	41.0
24	36.824990239798176	39.0	35.0	40.0	30.0	41.0
25	36.69266316287232	38.0	35.0	40.0	30.0	41.0
26	36.45224265601183	38.0	35.0	40.0	30.0	41.0
27	36.324185586855485	38.0	35.0	40.0	30.0	41.0
28	36.15235578415974	38.0	35.0	40.0	30.0	41.0
29	35.933306575992354	38.0	34.0	40.0	29.0	41.0
30	35.696589147686346	38.0	34.0	40.0	28.0	41.0
31	35.42532318186445	38.0	34.0	40.0	27.0	41.0
32	35.30101493214244	37.0	34.0	40.0	27.0	41.0
33	35.11147567809244	37.0	33.0	40.0	27.0	41.0
34	35.2400197909835	38.0	34.0	40.0	27.0	41.0
35	35.142457071217905	38.0	33.0	40.0	26.0	41.0
36	35.07337545789198	38.0	33.0	40.0	26.0	41.0
37	34.936631486990905	38.0	33.0	40.0	25.0	41.0
38	34.889484236146636	38.0	33.0	40.0	25.0	41.0
39	34.75943776083576	38.0	33.0	40.0	24.0	41.0
40	34.55071954913666	38.0	33.0	40.0	23.0	41.0
41	34.411487532066936	38.0	33.0	40.0	23.0	41.0
42	34.216758942986246	38.0	33.0	40.0	22.0	41.0
43	33.829834907635636	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	5.0
15	5.0
16	22.0
17	47.0
18	152.0
19	295.0
20	654.0
21	1300.0
22	2365.0
23	4253.0
24	7007.0
25	10813.0
26	16211.0
27	23432.0
28	32474.0
29	43286.0
30	55173.0
31	66815.0
32	80171.0
33	97152.0
34	115867.0
35	137187.0
36	168073.0
37	212194.0
38	248018.0
39	229250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.201916993832064	22.67330317441706	14.78976589688846	22.33501393486241
2	18.23057526565143	24.161299092526715	36.08736379203491	21.52076184978695
3	19.454691403678083	25.476123904956893	33.82918165056287	21.24000304080215
4	13.123831513791199	16.58248626807248	36.80845909927832	33.485223118858
5	11.669593653485133	39.02702061947325	35.70159423072215	13.601791496319471
6	31.017921405572142	37.78654084274028	13.62768985364207	17.56784789804551
7	23.81476360984447	34.85899568489559	22.162809185799453	19.163431519460488
8	26.067856273136186	34.69864491032855	19.73841370628686	19.495085110248407
9	23.633861651232877	14.63205649707323	23.589344823098756	38.14473702859514
10	15.736344414652029	27.81728386790034	34.48862340567264	21.957748311774992
11	31.527642308896535	22.72658163587425	25.085007170366097	20.660768884863117
12	20.855586378752523	27.58845062110961	31.843963041369083	19.711999958768782
13	31.38752059950188	21.6298957236787	27.94864394397193	19.033939732847493
14	19.693123792859527	23.73159251704975	29.276482358837846	27.298801331252875
15	21.03629506604081	30.14034075022774	27.48949570357848	21.33386848015297
16	20.568578463647597	29.24040504515462	28.22850082011465	21.96251567108313
17	20.389287099396864	27.09831454521325	27.896589534228994	24.61580882116089
18	22.53047566649616	25.452673651062803	31.671436173433953	20.345414509007085
19	21.56450559262786	24.650275540483253	32.992767787082	20.792451079806884
20	22.821220160518276	24.081478036002583	33.085087055846394	20.012214747632747
21	21.66313839128681	24.999967788112784	33.870026323554235	19.46686749704617
22	19.578578321915295	25.416016523409667	33.17019086187414	21.8352142928009
23	21.17976681170606	25.738457514453472	34.385931909224325	18.695843764616143
24	21.036552761138548	25.526438872790102	31.585688129661865	21.85132023640948
25	20.55195712984354	26.488865639064514	32.63180137892647	20.327375852165478
26	20.476130347334337	27.284628100877324	32.367857175069034	19.87138437671931
27	18.830296181860582	27.34608838168767	32.16910983093913	21.654505605512615
28	19.819780933397414	26.52855068411606	32.17368391892397	21.477984463562557
29	20.06356049585691	26.364721025729565	31.158622928936712	22.413095549476814
30	20.353274209488077	26.702623722637615	33.37344787021444	19.57065419765987
31	21.701921503496276	26.202888504350536	32.958687610406244	19.136502381746944
32	20.814226315565687	26.43668238177271	31.488472654040468	21.260618648621136
33	20.37923699058511	26.34990355760967	32.321600905025186	20.949258546780037
34	19.166008470437863	27.26401249305834	33.344908138140035	20.225070898363764
35	18.63148441395625	29.784979210447993	31.59451418675937	19.98902218883639
36	21.597941531559275	26.877083303805772	31.230455437430987	20.29451972720397
37	20.543968581813683	28.146553779034182	30.98674029874593	20.322737340406206
38	20.43599433586175	26.16642464802071	32.430670355142496	20.966910660975042
39	19.93187830091314	25.701220572830437	33.302324023238945	21.06457710301748
40	18.16621591499154	26.460068211892374	32.874485737220574	22.49923013589551
41	20.488306440702424	25.349015797997964	32.71516574304449	21.44751201825512
42	18.813352729184356	28.012423480661912	31.99162233237256	21.182601457781168
43	19.29640218989294	28.273726309767543	30.54028354191604	21.889587958423473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	162.0
2	231.0
3	633.0
4	1035.0
5	1035.0
6	1809.0
7	2583.0
8	2991.5
9	3400.0
10	5200.0
11	7000.0
12	7000.0
13	13184.0
14	19368.0
15	32665.0
16	45962.0
17	44833.5
18	43705.0
19	43705.0
20	45487.0
21	47269.0
22	38843.0
23	30417.0
24	33279.5
25	36142.0
26	36142.0
27	38915.5
28	41689.0
29	43021.5
30	44354.0
31	47813.5
32	51273.0
33	51273.0
34	56342.0
35	61411.0
36	66273.5
37	71136.0
38	74105.0
39	77074.0
40	77074.0
41	79219.0
42	81364.0
43	84228.0
44	87092.0
45	95117.5
46	103143.0
47	103143.0
48	144024.0
49	184905.0
50	167392.5
51	149880.0
52	132036.5
53	114193.0
54	114193.0
55	96711.5
56	79230.0
57	64201.0
58	49172.0
59	42817.5
60	36463.0
61	36463.0
62	31731.5
63	27000.0
64	23087.5
65	19175.0
66	16204.5
67	13234.0
68	13234.0
69	11131.5
70	9029.0
71	7412.5
72	5796.0
73	4724.5
74	3653.0
75	3653.0
76	2916.0
77	2179.0
78	1714.5
79	1250.0
80	962.5
81	675.0
82	675.0
83	509.0
84	343.0
85	269.0
86	195.0
87	126.5
88	58.0
89	58.0
90	46.5
91	35.0
92	23.0
93	11.0
94	7.0
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1552222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.94676147259519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36980244405703	36.32261660522958
2	12.823388303284435	12.040331038895232
3	4.162532357275531	5.862522410969212
4	1.8072586564275368	3.3937976425034577
5	0.9808765257422651	2.3024488144045
6	0.5784214721051618	1.6293008928929043
7	0.3885885431555546	1.277008155255496
8	0.27071318542621153	1.016728587495263
9	0.20382068881095178	0.8611849134709024
>10	1.2055431220452284	10.764151360024073
>50	0.12124642646294756	3.9653194388078266
>100	0.07416151629984721	6.486327298627661
>500	0.005513841982840088	1.8663591456786797
>1k	0.006754456428979108	5.463306927756085
>5k	9.649223469970154E-4	2.7722293024474087
>10k+	4.1353814871300667E-4	3.976367465541833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21392	1.3781533826991241	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21358	1.375962974368357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18695	1.204402463049744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7213	0.464688684994801	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6331	0.40786691594372454	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6263	0.4034860992821903	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5977	0.38506089979397273	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5938	0.382548372591034	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5634	0.3629635451629986	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5482	0.3531711314489809	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3956	0.25486045166219784	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3465	0.22322837841494322	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3243	0.2089263004905226	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3147	0.20274161814482725	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2983	0.1921761191375976	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2749	0.17710095591996505	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2724	0.17549036155910688	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2255	0.1452756113494075	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2249	0.14488906870280155	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2240	0.14430925473289258	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2213	0.14256981282316575	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2013	0.12968505793630036	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2010	0.12949178661299737	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1918	0.12356479936503928	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1882	0.1212455434854035	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1794	0.11557625133518272	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1725	0.11113101089921416	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1697	0.10932714521505299	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1593	0.10262707267388299	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1554	0.10011454547094424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.442377443432705E-5	0.0
2	0.0	0.0	0.0	6.442377443432705E-5	0.0
3	0.0	0.0	0.0	6.442377443432705E-5	0.0
4	0.0	0.0	0.0	6.442377443432705E-5	0.0
5	0.0	0.0	0.0	6.442377443432705E-5	0.0
6	0.0	0.0	0.0	6.442377443432705E-5	0.0
7	0.0	0.0	0.0	6.442377443432705E-5	6.442377443432705E-5
8	0.0	0.0	0.0	6.442377443432705E-5	6.442377443432705E-5
9	0.0	0.0	0.0	6.442377443432705E-5	6.442377443432705E-5
10	0.0	0.0	0.0	2.576950977373082E-4	6.442377443432705E-5
11	0.0	0.0	0.0	5.153901954746164E-4	6.442377443432705E-5
12	0.0	0.0	0.0	0.0010307803909492329	1.9327132330298113E-4
13	6.442377443432705E-5	0.0	0.0	0.0014173230375551951	1.9327132330298113E-4
14	6.442377443432705E-5	0.0	0.0	0.0016750181352925032	1.9327132330298113E-4
15	1.9327132330298113E-4	0.0	0.0	0.0019971370074641386	1.9327132330298113E-4
16	1.9327132330298113E-4	0.0	0.0	0.0030279173984133715	1.9327132330298113E-4
17	1.9327132330298113E-4	0.0	0.0	0.004702935533705875	1.9327132330298113E-4
18	1.9327132330298113E-4	0.0	0.0	0.005798139699089435	1.9327132330298113E-4
19	1.9327132330298113E-4	0.0	0.0	0.007473157834381938	1.9327132330298113E-4
20	1.9327132330298113E-4	0.0	0.0	0.010952041653835598	1.9327132330298113E-4
21	1.9327132330298113E-4	0.0	0.0	0.017780961743874266	1.9327132330298113E-4
22	1.9327132330298113E-4	0.0	0.0	0.026735866390245725	2.576950977373082E-4
23	1.9327132330298113E-4	0.0	0.0	0.03324266760811276	2.576950977373082E-4
24	1.9327132330298113E-4	0.0	0.0	0.041360063186837964	2.576950977373082E-4
25	1.9327132330298113E-4	0.0	0.0	0.04677166023932144	2.576950977373082E-4
26	1.9327132330298113E-4	0.0	0.0	0.056306378855601844	2.576950977373082E-4
27	1.9327132330298113E-4	0.0	0.0	0.13509665498878382	3.2211887217163524E-4
28	1.9327132330298113E-4	0.0	0.0	0.23830354163257575	3.2211887217163524E-4
29	1.9327132330298113E-4	0.0	0.0	0.3547817258098391	3.2211887217163524E-4
30	2.576950977373082E-4	0.0	0.0	0.5633859074281901	3.2211887217163524E-4
31	2.576950977373082E-4	0.0	0.0	0.8445312590595933	3.2211887217163524E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5285	0.0	31.329233	1
GTCGTCA	55	1.9032159E-5	23.545454	24
CGCGATA	60	9.238803E-4	18.5	14
TTGGACC	755	0.0	17.887419	4
ATTGGAC	385	0.0	17.779222	3
GTATCAA	9380	0.0	17.750534	2
ACGTTTA	85	2.724112E-5	17.411764	26
TACGTGC	75	2.0678541E-4	17.266666	8
GTATTGG	375	0.0	16.773333	1
GGACCCT	790	0.0	16.626581	6
CAAACGA	135	2.2208042E-8	16.444445	23
CGAACTA	80	3.3835217E-4	16.1875	24
CTGTGCG	105	9.347243E-6	15.857142	9
TGGACCC	880	0.0	15.767045	5
CTAATAC	235	0.0	15.744681	3
TGCGCGA	95	7.0622205E-5	15.578948	12
ATAGCGG	145	5.3487383E-8	15.310345	37
CGAGTAT	75	0.004105267	14.8	10
TAGGTCG	75	0.004105267	14.8	21
TAGGCTC	100	1.093756E-4	14.8	5
>>END_MODULE