FastQCFastQC Report
Wed 25 May 2016
SRR2088309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098683
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT485661.5673110156798873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT477701.541622682926908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT421771.3611266463849319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163820.5286762150242539No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG147950.4774609083923719No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG146730.47352375186490525No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA146210.47184561957450954No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA140430.4531925337312659No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG138090.44564093842448554No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA132080.42624560176048987No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA92490.29848164526671495No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA81640.26346676959211385No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA76960.24836357897855313No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC76340.2463627289400045No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA69700.22493427046264494No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA63700.2055712055734646No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60520.19530878118219902No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG56560.18252915835534No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG52400.16910410003217496No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA51020.1646505951076635No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG51000.16458605155803288No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49220.1588416756409094No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT47150.15216141825414214No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG44210.1426735164584438No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC43800.1413503736910165No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.13573508487315417No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT40170.12963571943306237No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT40110.12944208878417057No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA38900.1255372040315192No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG36830.11885694664475197No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT35460.1144357134950558No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.11401618042245688No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT35100.11327392960170499No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA34680.11191851505946235No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA34610.11169261263575525No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34350.11085354649055744No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA34290.11065991584166562No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT33670.108659065803117No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG32800.10585142139418585No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA32160.10378602780600661No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA31680.10223698261487218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115500.031.4259761
ACGTTTA1400.023.78571326
GTATTGG10400.022.9471151
ATTGGAC12150.020.7078193
TCACGTT1700.019.58823624
GGACCCT19850.019.5717896
TTGGACC21550.018.8004634
CAATGCG1409.458745E-1118.519
TGGACCC21550.018.1995355
GACCCTC21450.018.1981357
GTATCAA205850.017.6956522
GATACCT15000.016.03333536
TATTGGA15400.015.8571422
TATACCG2003.6379788E-1215.7255
TTTTTAC24350.015.4989731
CGCATAC1101.4533696E-515.13636437
GCGAATA750.004106495514.829
TACTGTG4700.014.1702147
TTGATAC43450.014.13578834
TGATACC29250.013.977777535
Produced by FastQC (version 0.11.5)