##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3098683
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9193557391963	31.0	31.0	33.0	28.0	34.0
2	31.235312550525496	31.0	31.0	34.0	28.0	34.0
3	31.281826505002286	31.0	31.0	34.0	28.0	34.0
4	35.176631168790095	35.0	35.0	37.0	33.0	37.0
5	34.9509197939899	35.0	35.0	37.0	32.0	37.0
6	35.012556302145136	36.0	35.0	37.0	32.0	37.0
7	34.81464802950156	35.0	35.0	37.0	32.0	37.0
8	34.88867076754867	36.0	35.0	37.0	32.0	37.0
9	36.33370757834861	38.0	35.0	39.0	32.0	39.0
10	36.15876583697009	38.0	35.0	39.0	31.0	39.0
11	36.2478578802672	38.0	35.0	39.0	32.0	39.0
12	36.01325046802141	38.0	35.0	39.0	30.0	39.0
13	36.086247609064884	38.0	35.0	39.0	31.0	39.0
14	36.86284527975272	38.0	36.0	40.0	31.0	41.0
15	36.988289541072774	38.0	36.0	40.0	31.0	41.0
16	36.947558688642886	38.0	36.0	40.0	31.0	41.0
17	37.07306329818184	39.0	36.0	40.0	31.0	41.0
18	37.09169927998443	39.0	36.0	40.0	31.0	41.0
19	37.19314140878561	39.0	36.0	40.0	31.0	41.0
20	37.18136124282477	39.0	36.0	40.0	31.0	41.0
21	37.10485196452816	39.0	36.0	40.0	31.0	41.0
22	37.038493127564195	39.0	36.0	40.0	31.0	41.0
23	36.90495768686245	39.0	35.0	40.0	31.0	41.0
24	36.81825891838565	39.0	35.0	40.0	30.0	41.0
25	36.68042068194778	38.0	35.0	40.0	30.0	41.0
26	36.42953925909814	38.0	35.0	40.0	30.0	41.0
27	36.309732231402826	38.0	35.0	40.0	30.0	41.0
28	36.120802934666116	38.0	35.0	40.0	30.0	41.0
29	35.893117172682715	38.0	34.0	40.0	29.0	41.0
30	35.64707425703113	38.0	34.0	40.0	28.0	41.0
31	35.34693900602288	38.0	34.0	40.0	27.0	41.0
32	35.22706485303595	37.0	34.0	40.0	27.0	41.0
33	35.01996945153796	37.0	33.0	40.0	26.0	41.0
34	35.13021790225073	38.0	33.0	40.0	27.0	41.0
35	35.02278645476159	38.0	33.0	40.0	26.0	41.0
36	34.93201918363382	38.0	33.0	40.0	25.0	41.0
37	34.78934082640915	38.0	33.0	40.0	25.0	41.0
38	34.704003281394066	38.0	33.0	40.0	24.0	41.0
39	34.56599368183193	38.0	33.0	40.0	24.0	41.0
40	34.337855792283364	38.0	33.0	40.0	23.0	41.0
41	34.17116013480566	38.0	33.0	40.0	21.0	41.0
42	33.963459960247626	37.0	33.0	40.0	21.0	41.0
43	33.56941223093811	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	6.0
15	9.0
16	39.0
17	108.0
18	291.0
19	648.0
20	1488.0
21	2917.0
22	5211.0
23	8973.0
24	14620.0
25	22663.0
26	34068.0
27	48817.0
28	67608.0
29	88845.0
30	112455.0
31	135164.0
32	162637.0
33	194192.0
34	230871.0
35	271263.0
36	334387.0
37	424612.0
38	489470.0
39	447316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52928292439078	22.542383328659305	14.901976097587266	22.026357649362648
2	18.42795794213219	24.110081605636978	36.154940663501236	21.3070197887296
3	19.553242458166906	25.198350395958542	34.33203719128417	20.916369954590387
4	12.901126059038631	16.2882101847785	36.790468724938954	34.020195031243915
5	11.391484704953685	39.072696368102186	35.75238254445517	13.783436382488947
6	30.966058806273505	37.51093609769053	13.101243334668307	18.42176176136765
7	23.81963563229927	35.15493517729952	21.574940063246224	19.450489127154988
8	26.43665066739644	34.32693825086335	19.420024571729343	19.816386510010865
9	23.45644907852788	14.51168125297102	23.909673884033957	38.122195784467145
10	15.984855501514675	27.5679377335468	34.28011190560635	22.167094859332174
11	31.192961654999884	22.37534462221531	25.54440063730301	20.8872930854818
12	21.24260532619826	27.551575943715445	31.583546945589465	19.622271784496835
13	31.832685047163583	21.3747259722921	28.253390230623783	18.53919874992053
14	19.74961620791801	23.75631841011165	29.386549059713435	27.10751632225691
15	20.859023010743595	29.858911027684986	28.13027340970341	21.151792551868002
16	20.38617696614981	29.16364791106415	28.51059627590173	21.939578846884306
17	20.37933534988897	26.699149283744095	27.85409156083407	25.06742380553287
18	22.71113243916851	24.898932869222183	32.158726788122564	20.23120790348674
19	21.626349000526996	23.975508304657172	33.657815271842914	20.74032742297292
20	23.09116485939349	23.256009085150044	33.884944022993	19.76788203246347
21	21.697121002696953	24.364641365380066	34.70638977914165	19.23184785278133
22	19.14858667375785	24.92084540432177	33.987955528203436	21.942612393716942
23	21.201007008461335	25.2256845892271	35.47933105774292	18.093977344568643
24	20.97065107982972	24.969672599617322	31.987202304979245	22.07247401557371
25	20.38395021368756	25.979585520687337	33.52395194990904	20.112512315716064
26	20.28148732864898	26.95335405396422	33.152181104036785	19.612977513350028
27	18.343147717917578	27.203202134584277	32.70331298813076	21.750337159367383
28	19.515290850984112	26.11361020149528	32.82323490334442	21.547864044176187
29	19.84594745574168	25.999239031549852	31.60574992666239	22.549063586046074
30	20.25373360230782	26.22685185932217	34.193623549101346	19.32579098926867
31	21.77095882347436	25.749810484002396	33.68347133282107	18.79575935970217
32	20.766854821871096	25.945829244230534	31.947572565506054	21.339743368392313
33	20.196483473785477	25.865569340264884	33.068145402417734	20.86980178353191
34	18.811669344686113	27.126685756497192	34.03036064031074	20.031284258505952
35	18.32433327320026	30.054316624191635	31.949379784895708	19.671970317712393
36	21.80206881439631	26.735325943312045	31.45807428510758	20.00453095718407
37	20.633378761235015	28.12133412807957	31.142746773387277	20.102540337298137
38	20.311435535677578	25.75726526398473	33.04829826090633	20.883000939431366
39	19.74280686343198	25.47088553427375	33.75143569058209	21.03487191171217
40	17.862750078017015	26.352356791578874	33.20275097517236	22.582142155231754
41	20.51339230247173	25.034377508121995	33.160442678389494	21.29178751101678
42	18.61355291909498	28.147022460832556	32.200389649409125	21.03903497066334
43	19.196574802908202	28.432401765524258	30.66731898680827	21.70370444475927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	234.0
1	336.0
2	438.0
3	1293.5
4	2149.0
5	2149.0
6	3857.5
7	5566.0
8	6445.0
9	7324.0
10	11299.0
11	15274.0
12	15274.0
13	28772.5
14	42271.0
15	71311.0
16	100351.0
17	97236.5
18	94122.0
19	94122.0
20	98917.5
21	103713.0
22	82710.5
23	61708.0
24	66040.0
25	70372.0
26	70372.0
27	74311.5
28	78251.0
29	79473.5
30	80696.0
31	87491.0
32	94286.0
33	94286.0
34	102505.0
35	110724.0
36	121893.5
37	133063.0
38	138318.5
39	143574.0
40	143574.0
41	148879.5
42	154185.0
43	161147.0
44	168109.0
45	186538.5
46	204968.0
47	204968.0
48	300577.0
49	396186.0
50	357559.0
51	318932.0
52	278190.5
53	237449.0
54	237449.0
55	198857.5
56	160266.0
57	125738.0
58	91210.0
59	78900.5
60	66591.0
61	66591.0
62	58306.0
63	50021.0
64	43289.0
65	36557.0
66	31007.5
67	25458.0
68	25458.0
69	21477.5
70	17497.0
71	14486.0
72	11475.0
73	9302.5
74	7130.0
75	7130.0
76	5633.0
77	4136.0
78	3182.0
79	2228.0
80	1678.5
81	1129.0
82	1129.0
83	838.0
84	547.0
85	409.5
86	272.0
87	198.0
88	124.0
89	124.0
90	99.5
91	75.0
92	44.5
93	14.0
94	10.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3098683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.15048753433181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89376839553077	25.27101009783242
2	13.825612610335094	9.719540474281688
3	5.576284310106919	5.880273363909099
4	2.693249197735979	3.786760894074707
5	1.5475995702608925	2.7199439701296377
6	0.9249699750888062	1.950788734739417
7	0.6235984962355443	1.5343853817848894
8	0.41856765545167685	1.1770285724182925
9	0.30681791100571915	0.9706319240504632
>10	1.8162146242044324	12.277671794009779
>50	0.20260685261176575	4.9772482866519985
>100	0.14795192600280813	9.88778113111633
>500	0.012489407247909937	2.989747170734749
>1k	0.008326271498605828	6.669681140743247
>5k	0.0010176554053851566	2.3814761529987822
>10k+	9.251412776228697E-4	7.806030910524485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	48566	1.5673110156798873	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47770	1.541622682926908	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	42177	1.3611266463849319	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16382	0.5286762150242539	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14795	0.4774609083923719	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14673	0.47352375186490525	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14621	0.47184561957450954	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14043	0.4531925337312659	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13809	0.44564093842448554	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13208	0.42624560176048987	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9249	0.29848164526671495	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8164	0.26346676959211385	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7696	0.24836357897855313	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7634	0.2463627289400045	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6970	0.22493427046264494	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6370	0.2055712055734646	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6052	0.19530878118219902	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5656	0.18252915835534	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5240	0.16910410003217496	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5102	0.1646505951076635	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5100	0.16458605155803288	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4922	0.1588416756409094	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4715	0.15216141825414214	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4421	0.1426735164584438	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4380	0.1413503736910165	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4206	0.13573508487315417	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4017	0.12963571943306237	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4011	0.12944208878417057	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3890	0.1255372040315192	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3683	0.11885694664475197	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3546	0.1144357134950558	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3533	0.11401618042245688	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	3510	0.11327392960170499	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	3468	0.11191851505946235	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	3461	0.11169261263575525	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3435	0.11085354649055744	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3429	0.11065991584166562	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3367	0.108659065803117	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3280	0.10585142139418585	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3216	0.10378602780600661	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3168	0.10223698261487218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2271774815300566E-5	0.0
3	0.0	0.0	0.0	3.2271774815300566E-5	0.0
4	0.0	0.0	0.0	3.2271774815300566E-5	0.0
5	3.2271774815300566E-5	0.0	0.0	3.2271774815300566E-5	0.0
6	1.2908709926120226E-4	0.0	0.0	3.2271774815300566E-5	0.0
7	1.2908709926120226E-4	0.0	0.0	3.2271774815300566E-5	0.0
8	1.9363064889180338E-4	0.0	0.0	3.2271774815300566E-5	0.0
9	1.9363064889180338E-4	0.0	0.0	1.6135887407650284E-4	0.0
10	1.9363064889180338E-4	0.0	0.0	2.904459733377051E-4	0.0
11	1.9363064889180338E-4	0.0	0.0	6.777072711213119E-4	0.0
12	1.9363064889180338E-4	0.0	0.0	0.001258599217796722	0.0
13	1.9363064889180338E-4	0.0	0.0	0.0015167734163191266	0.0
14	1.9363064889180338E-4	0.0	0.0	0.0018717629392874327	0.0
15	1.9363064889180338E-4	0.0	0.0	0.0025494702104087445	0.0
16	1.9363064889180338E-4	0.0	0.0	0.0031949057067147558	0.0
17	1.9363064889180338E-4	0.0	0.0	0.004163058951173773	0.0
18	1.9363064889180338E-4	0.0	0.0	0.005034396871186888	0.0
19	1.9363064889180338E-4	0.0	0.0	0.006357539638614212	0.0
20	2.2590242370710396E-4	0.0	0.0	0.009810619543851372	0.0
21	2.2590242370710396E-4	0.0	0.0	0.018265824545460118	0.0
22	2.2590242370710396E-4	0.0	0.0	0.028850966684878705	0.0
23	2.2590242370710396E-4	0.0	0.0	0.03556349584646122	0.0
24	2.2590242370710396E-4	0.0	0.0	0.04440596214585358	0.0
25	2.2590242370710396E-4	0.0	0.0	0.050118066288161775	0.0
26	2.2590242370710396E-4	0.0	0.0	0.06109046972536397	0.0
27	2.2590242370710396E-4	0.0	0.0	0.14386757212660992	0.0
28	2.2590242370710396E-4	0.0	0.0	0.25194574598305153	0.0
29	2.2590242370710396E-4	0.0	0.0	0.37496575157897727	0.0
30	2.2590242370710396E-4	0.0	0.0	0.5908639250933381	0.0
31	2.2590242370710396E-4	0.0	0.0	0.8677557530086168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11550	0.0	31.425976	1
ACGTTTA	140	0.0	23.785713	26
GTATTGG	1040	0.0	22.947115	1
ATTGGAC	1215	0.0	20.707819	3
TCACGTT	170	0.0	19.588236	24
GGACCCT	1985	0.0	19.571789	6
TTGGACC	2155	0.0	18.800463	4
CAATGCG	140	9.458745E-11	18.5	19
TGGACCC	2155	0.0	18.199535	5
GACCCTC	2145	0.0	18.198135	7
GTATCAA	20585	0.0	17.695652	2
GATACCT	1500	0.0	16.033335	36
TATTGGA	1540	0.0	15.857142	2
TATACCG	200	3.6379788E-12	15.725	5
TTTTTAC	2435	0.0	15.498973	1
CGCATAC	110	1.4533696E-5	15.136364	37
GCGAATA	75	0.0041064955	14.8	29
TACTGTG	470	0.0	14.170214	7
TTGATAC	4345	0.0	14.135788	34
TGATACC	2925	0.0	13.9777775	35
>>END_MODULE