FastQCFastQC Report
Wed 25 May 2016
SRR2088308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335604
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT511241.5326759411488893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT494551.4826400256145515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT437541.3117264519409377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172200.5162483316364892No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA166540.49927989053856514No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG166060.4978408707988119No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG145750.43695234806050115No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA144770.43401434942517153No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA141860.4252902922529173No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG137060.41090009485538453No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA94930.2845961331141227No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA82900.24853070088655607No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA75170.22535648716094595No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC72560.21753181732603752No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA69440.20817818901764118No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA63110.1892011161996448No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62670.18788201477153763No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG61040.1829953435719588No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG58790.17624993854186527No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG58570.1755903878278117No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC51990.15586382556202716No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC51370.1540050917315125No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49680.1489385430644645No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT48210.14453154511147007No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG44350.13295942803762076No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.12426535044327804No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA38430.11521151791399697No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37680.1129630495706325No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT37460.1123034988565789No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.11146407067505616No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA37040.11104435658429478No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG36310.10885584739675333No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA35660.1069071748325041No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT35350.10597780791724677No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT34670.10393919661926296No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC34440.10324966632729783No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT34310.102859931814448No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA34270.10274001350280189No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA34060.10211044236665982No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG33460.10031166769196823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120300.030.5565261
GTATTGG12750.021.6196081
ATTGGAC14550.019.7079053
TTGGACC21700.017.9032274
GGACCCT20900.017.5263166
GTATCAA211250.017.488522
TGGACCC21600.017.129635
GACCCTC22000.016.3977287
TATTGGA17200.016.0261632
GATACCT15000.015.78666636
CGACGAG1557.223207E-915.51612924
CGGTTAC855.367951E-415.23529431
TTGATAC38600.015.04922334
CGTTTAG1853.0559022E-1015.026
ATAAGCG1252.9626462E-614.8000013
TATACCG2151.2732926E-1114.6279075
TGATACC28700.014.18118535
CGAATTA2353.6379788E-1214.17021215
GTATTAG5150.014.0097091
GATACTG12600.013.94841336
Produced by FastQC (version 0.11.5)