FastQCFastQC Report
Wed 25 May 2016
SRR2088307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138807
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369991.1787599556137092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364991.1628303364940884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314091.0006668138563473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129250.4117806542422009No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA119260.3799532752411983No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG111770.3560907058000062No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG101400.32305267574591234No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA98300.3131763118917474No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA96310.30683632348213824No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG92270.2939651912334846No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66640.21230996362630772No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA57860.18433755245225336No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC51140.16292814435548283No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51100.16280070740252586No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46820.14916495343613034No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46030.14664807361523025No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43360.13814165700535266No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40820.13004941049258525No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG40300.12839273010414465No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38410.12237133407692795No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35930.11447024299359597No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35920.11443838375535674No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33840.10781166220159442No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31960.10182212541261697No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31790.10128051836254985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86150.029.2692991
GTATTGG10700.021.2663561
ATTGGAC12250.019.4816323
TTGGACC17450.018.128944
GGACCCT17900.017.1564246
GTATCAA151600.016.7549482
TATACTG5150.016.5242735
GTATTAG4100.016.2439041
TGGACCC19750.016.2050635
GCGAACG700.002593834715.85714231
TATTGGA16000.015.7252
CACGGTA957.065899E-515.57894832
ACGTTTA1902.7284841E-1115.57894826
TATACCG2550.015.2352945
CCGACCG2450.015.102049
CGTGTCT3350.014.91044835
CGGTATA2006.184564E-1114.826
GACCCTC20750.014.7108437
TGCGACG1904.5656634E-1014.60526422
CAATGCG1803.3378456E-914.38888919
Produced by FastQC (version 0.11.5)