FastQCFastQC Report
Wed 25 May 2016
SRR2088306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646229
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT442391.6717751940591687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT429531.6231777370741534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT380561.438121946362163No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA158780.6000236563048776No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG146550.5538069456573864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144950.5477606057525634No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG127680.4824979244048795No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA127320.48113749792629434No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG123190.46553038304696986No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA121810.46031541487905997No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA89600.33859503467009094No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA73300.2769979468897061No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA69410.262297782996105No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC67320.25439975149542987No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA61920.2339933543166521No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG54920.20754061723305123No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA54830.20720051061340494No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG54400.20557555676398379No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG52790.19949142723475555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.19688394315080066No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC46920.177308917708936No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC46150.17439911662973992No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43520.164460445411187No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT42660.16121053771234461No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG36900.13944371405498165No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.12992072870488533No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA33720.12742661349414583No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG33220.1255371322738886No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC33080.1250080775322166No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA32770.12383659917565713No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT32610.12323196518517483No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA32600.1231941755607697No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT32550.12300522743874395No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA32190.12164480096015877No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC31710.11983089898871185No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31660.11964195086668615No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30830.11650541204105919No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG30250.11431361382556082No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT30190.11408687607912997No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA30100.11374676945948366No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT29940.11314213546900136No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA29320.11079917875588244No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG29200.1103457032630207No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT28490.10766263993025547No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA27960.10565978983678283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC27570.10418599448498221No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC26710.10093608678613981No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA26660.1007471386641141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.030.1745971
GTATTGG12150.022.991771
TCTATAC1750.022.23
ATTGGAC12450.021.6947783
TTGGACC20500.019.4926834
TAAACGT1750.019.0285724
TATTGGA15100.018.7450332
ACGTTTA1700.018.526
TGGACCC21950.018.1207285
ACGCGAA1054.803096E-717.61904734
GGACCCT22200.017.5833346
TGACGCG852.7252863E-517.41176432
GTATCAA194050.017.3893322
CATTGCG1201.0426811E-716.95833429
TCCGGTC2950.016.9322038
GACCCTC23050.016.3731027
TCACGTA2850.016.2280725
TAGACTA1751.3278623E-1015.8571435
TACCCGG1059.352221E-615.85714220
GTCGTCA1302.5924965E-715.65384624
Produced by FastQC (version 0.11.5)