##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2646229
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.928684932407588	31.0	31.0	33.0	28.0	34.0
2	31.238271139799313	31.0	31.0	34.0	28.0	34.0
3	31.280884609759774	31.0	31.0	34.0	28.0	34.0
4	35.175430773376	35.0	35.0	37.0	33.0	37.0
5	34.96044635592762	35.0	35.0	37.0	32.0	37.0
6	35.02262615971634	36.0	35.0	37.0	32.0	37.0
7	34.82318385899331	35.0	35.0	37.0	32.0	37.0
8	34.904199145274276	36.0	35.0	37.0	32.0	37.0
9	36.3499848274658	38.0	35.0	39.0	32.0	39.0
10	36.17347478241679	38.0	35.0	39.0	31.0	39.0
11	36.2721638981358	38.0	35.0	39.0	32.0	39.0
12	36.023708076663056	38.0	35.0	39.0	30.0	39.0
13	36.09725235419913	38.0	35.0	39.0	31.0	39.0
14	36.86293816597127	39.0	36.0	40.0	31.0	41.0
15	36.99001409175094	38.0	36.0	40.0	31.0	41.0
16	36.94289496487266	38.0	36.0	40.0	31.0	41.0
17	37.08109388869973	39.0	36.0	40.0	31.0	41.0
18	37.100109249804156	39.0	36.0	40.0	31.0	41.0
19	37.200040132581115	39.0	36.0	40.0	31.0	41.0
20	37.190540576798156	39.0	36.0	40.0	31.0	41.0
21	37.11207004382463	39.0	36.0	40.0	31.0	41.0
22	37.04063707260407	39.0	36.0	40.0	31.0	41.0
23	36.907502714239776	39.0	35.0	40.0	31.0	41.0
24	36.8234797517524	39.0	35.0	40.0	30.0	41.0
25	36.69431783870557	38.0	35.0	40.0	30.0	41.0
26	36.43611116044757	38.0	35.0	40.0	30.0	41.0
27	36.31695631783946	38.0	35.0	40.0	30.0	41.0
28	36.13035644307428	38.0	35.0	40.0	30.0	41.0
29	35.90276351744313	38.0	34.0	40.0	29.0	41.0
30	35.64037768462215	38.0	34.0	40.0	28.0	41.0
31	35.33517620735016	38.0	34.0	40.0	27.0	41.0
32	35.22327394945789	37.0	34.0	40.0	27.0	41.0
33	35.01261342083395	37.0	33.0	40.0	27.0	41.0
34	35.11781293304548	37.0	33.0	40.0	27.0	41.0
35	34.99186124859187	37.0	33.0	40.0	26.0	41.0
36	34.90004871082586	37.0	33.0	40.0	25.0	41.0
37	34.74783210372194	37.0	33.0	40.0	25.0	41.0
38	34.66070358990095	38.0	33.0	40.0	24.0	41.0
39	34.512292397974626	38.0	33.0	40.0	24.0	41.0
40	34.26198677438725	37.0	33.0	40.0	23.0	41.0
41	34.09405610776694	37.0	33.0	40.0	21.0	41.0
42	33.85459535059135	37.0	33.0	40.0	20.0	41.0
43	33.47473064500465	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	5.0
15	10.0
16	28.0
17	88.0
18	248.0
19	555.0
20	1160.0
21	2393.0
22	4366.0
23	7645.0
24	12025.0
25	19162.0
26	29142.0
27	41601.0
28	56955.0
29	76020.0
30	96183.0
31	115362.0
32	139135.0
33	167140.0
34	197983.0
35	234404.0
36	287989.0
37	369262.0
38	417630.0
39	369736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.889885191342096	22.48331493608452	14.86572023811998	21.761079634453406
2	18.34538885334565	24.143904401319766	36.439779021392326	21.07092772394226
3	19.84420093650247	25.117780811864733	34.46205902814911	20.575959223483682
4	12.734838897162717	16.07903170889594	36.63292179172702	34.55320760221432
5	11.43993962729605	39.12318246077721	35.63312169884013	13.803756213086624
6	31.28969563858608	37.05552316144974	12.792732601751396	18.862048598212777
7	24.139294067142337	35.13732182664464	21.19582243260126	19.52756167361177
8	26.698369642234287	34.14175417169111	19.01260246184287	20.147273724231727
9	23.324134079098975	14.595788950994038	23.90998662625192	38.17009034365507
10	15.962715244976907	27.441275868415016	34.19798513280597	22.39802375380211
11	31.181881840158205	22.261716578572756	25.587505843220676	20.96889573804837
12	21.63962378161527	27.29087316328254	31.67310916780067	19.396393887301514
13	32.637046907127086	20.854960020466862	28.222085087874106	18.285907984531953
14	19.890304278276748	23.712309100988616	29.27596213328476	27.121424487449875
15	20.88719457008445	29.82848423171237	27.950075371405873	21.3342458267973
16	20.447436710881785	29.192220325602964	28.521756809406895	21.838586154108356
17	20.626030475820496	26.465623345523003	27.58181548157775	25.32653069707875
18	23.21624470142229	24.37351415920542	32.024136988900054	20.386104150472235
19	21.6581784871982	23.510134610421094	33.86022146987278	20.97146543250792
20	23.46728117634566	22.746670828563968	34.020487266975	19.765560728115368
21	22.127790149680923	23.85058133668704	34.90302615533274	19.1186023582993
22	19.300257082814827	24.507705115468088	34.180904222574846	22.011133579142246
23	21.460841068554533	24.812062750427117	35.727520180604174	17.999576000414173
24	21.03400726089843	24.394903086618733	32.16516786718005	22.405921785302784
25	20.81955114239924	25.667733215832794	33.344468675991386	20.168246965776582
26	20.56110790109246	26.515089963869343	33.306452313839806	19.617349821198392
27	18.455999083979506	26.852362361685252	32.95640702297496	21.735231531360284
28	19.886714263958257	25.659041602219613	32.851843132245925	21.602401001576204
29	19.918155231463338	25.715045825588035	31.575498567962185	22.79130037498644
30	20.28796449589208	26.063163845608223	34.39411328346866	19.254758375031034
31	22.244219982473172	25.425161616776176	33.688543206200215	18.642075194550433
32	21.233838794752835	25.64830934888855	31.761158992664658	21.356692863693958
33	20.543157829500018	25.69603764451225	32.7995045024448	20.961300023542936
34	19.08119063013821	27.17191142565515	33.7530500950598	19.993847849146842
35	18.254731544397707	30.666998207638112	31.694573674462795	19.383696573501386
36	22.08539019109835	26.820052232818853	31.35677222190521	19.737785354177586
37	20.851445585397183	28.432346558064324	30.961795067622646	19.75441278891585
38	20.7009673010159	25.85426280189659	32.73027391053457	20.71449598655294
39	20.157779239816357	25.48112805052019	33.58809838453134	20.772994325132103
40	17.84584780833405	26.755129658090816	32.90841419998042	22.49060833359471
41	21.01447002508097	25.23447517202782	32.74251774884184	21.008537054049366
42	18.697890469796832	28.685234724583548	31.846299016449446	20.770575789170174
43	19.320436742247175	29.092040031304922	29.99823522454028	21.58928800190762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	227.5
2	328.0
3	989.5
4	1651.0
5	1651.0
6	3104.0
7	4557.0
8	5315.0
9	6073.0
10	9557.5
11	13042.0
12	13042.0
13	24890.5
14	36739.0
15	62676.0
16	88613.0
17	86029.5
18	83446.0
19	83446.0
20	87133.5
21	90821.0
22	71194.5
23	51568.0
24	55571.5
25	59575.0
26	59575.0
27	62545.0
28	65515.0
29	66973.0
30	68431.0
31	72282.0
32	76133.0
33	76133.0
34	82600.5
35	89068.0
36	97591.0
37	106114.0
38	110004.5
39	113895.0
40	113895.0
41	117530.5
42	121166.0
43	127150.0
44	133134.0
45	152316.0
46	171498.0
47	171498.0
48	259427.5
49	347357.0
50	316058.0
51	284759.0
52	249493.5
53	214228.0
54	214228.0
55	177905.5
56	141583.0
57	110940.5
58	80298.0
59	68779.5
60	57261.0
61	57261.0
62	50041.0
63	42821.0
64	37056.0
65	31291.0
66	26964.5
67	22638.0
68	22638.0
69	19455.0
70	16272.0
71	13629.5
72	10987.0
73	8963.5
74	6940.0
75	6940.0
76	5453.5
77	3967.0
78	3031.0
79	2095.0
80	1590.0
81	1085.0
82	1085.0
83	828.0
84	571.0
85	444.0
86	317.0
87	238.5
88	160.0
89	160.0
90	118.0
91	76.0
92	46.5
93	17.0
94	12.0
95	7.0
96	7.0
97	6.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2646229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.90442522470662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12877636542515	26.987754591521256
2	13.633106564332314	10.06243923567718
3	5.254560224942042	5.817495747302736
4	2.5132815199939	3.710048396930076
5	1.3619230860399236	2.5130494345281025
6	0.8301487659555816	1.8381697835154143
7	0.5417561912311453	1.3995240594518166
8	0.38654629167995275	1.1412214973752361
9	0.2861784651790144	0.9505126592208221
>10	1.7357408266707064	12.258257985197266
>50	0.18497371541661825	4.769151440727313
>100	0.12324807229875724	8.536818656458042
>500	0.008897132627232436	2.2308654524056584
>1k	0.008793677596683218	6.967106849982742
>5k	0.0010345503054921433	2.4075601061636522
>10k+	0.0010345503054921433	8.410024103542787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44239	1.6717751940591687	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42953	1.6231777370741534	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38056	1.438121946362163	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	15878	0.6000236563048776	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14655	0.5538069456573864	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14495	0.5477606057525634	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12768	0.4824979244048795	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12732	0.48113749792629434	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12319	0.46553038304696986	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12181	0.46031541487905997	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8960	0.33859503467009094	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7330	0.2769979468897061	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6941	0.262297782996105	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6732	0.25439975149542987	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6192	0.2339933543166521	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5492	0.20754061723305123	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5483	0.20720051061340494	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5440	0.20557555676398379	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5279	0.19949142723475555	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5210	0.19688394315080066	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4692	0.177308917708936	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4615	0.17439911662973992	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4352	0.164460445411187	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4266	0.16121053771234461	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3690	0.13944371405498165	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3438	0.12992072870488533	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3372	0.12742661349414583	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3322	0.1255371322738886	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3308	0.1250080775322166	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3277	0.12383659917565713	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3261	0.12323196518517483	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3260	0.1231941755607697	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3255	0.12300522743874395	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3219	0.12164480096015877	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	3171	0.11983089898871185	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3166	0.11964195086668615	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3083	0.11650541204105919	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3025	0.11431361382556082	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	3019	0.11408687607912997	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	3010	0.11374676945948366	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2994	0.11314213546900136	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2932	0.11079917875588244	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2920	0.1103457032630207	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	2849	0.10766263993025547	No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA	2796	0.10565978983678283	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2757	0.10418599448498221	No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC	2671	0.10093608678613981	No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA	2666	0.1007471386641141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.778962440514407E-5	0.0	0.0	0.0	0.0
6	3.778962440514407E-5	0.0	0.0	0.0	7.557924881028814E-5
7	7.557924881028814E-5	0.0	0.0	0.0	7.557924881028814E-5
8	1.511584976205763E-4	0.0	0.0	0.0	7.557924881028814E-5
9	1.511584976205763E-4	0.0	0.0	3.778962440514407E-5	7.557924881028814E-5
10	1.8894812202572037E-4	0.0	0.0	3.023169952411526E-4	7.557924881028814E-5
11	1.8894812202572037E-4	0.0	0.0	5.668443660771611E-4	7.557924881028814E-5
12	1.8894812202572037E-4	0.0	0.0	9.825302345337458E-4	1.1336887321543223E-4
13	1.8894812202572037E-4	0.0	0.0	0.0011714783565594663	1.1336887321543223E-4
14	1.8894812202572037E-4	0.0	0.0	0.001549374600610907	1.511584976205763E-4
15	1.8894812202572037E-4	0.0	0.0	0.00204063971787778	2.2673774643086446E-4
16	2.2673774643086446E-4	0.0	0.0	0.0028720114547909495	2.2673774643086446E-4
17	2.645273708360085E-4	0.0	0.0	0.0037789624405144074	2.2673774643086446E-4
18	2.645273708360085E-4	0.0	0.0	0.004459175679807001	2.2673774643086446E-4
19	2.645273708360085E-4	0.0	0.0	0.005706233285176755	2.2673774643086446E-4
20	3.023169952411526E-4	0.0	0.0	0.009485195725691163	2.2673774643086446E-4
21	3.023169952411526E-4	0.0	0.0	0.017005330982314833	2.2673774643086446E-4
22	3.023169952411526E-4	0.0	0.0	0.027397477693729456	2.2673774643086446E-4
23	3.023169952411526E-4	0.0	0.0	0.0341240308378451	2.2673774643086446E-4
24	3.023169952411526E-4	0.0	0.0	0.04372259543675169	2.2673774643086446E-4
25	3.023169952411526E-4	0.0	0.0	0.04905093247787701	2.2673774643086446E-4
26	3.023169952411526E-4	0.0	0.0	0.05880065557440418	2.2673774643086446E-4
27	3.023169952411526E-4	0.0	0.0	0.12258954157028738	2.2673774643086446E-4
28	3.023169952411526E-4	0.0	0.0	0.21456948737240805	2.2673774643086446E-4
29	3.401066196462967E-4	0.0	0.0	0.3240460292741104	2.2673774643086446E-4
30	3.7789624405144075E-4	0.0	0.0	0.5239909320017277	2.2673774643086446E-4
31	4.156858684565848E-4	0.0	0.0	0.7529582662724957	2.645273708360085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11140	0.0	30.174597	1
GTATTGG	1215	0.0	22.99177	1
TCTATAC	175	0.0	22.2	3
ATTGGAC	1245	0.0	21.694778	3
TTGGACC	2050	0.0	19.492683	4
TAAACGT	175	0.0	19.028572	4
TATTGGA	1510	0.0	18.745033	2
ACGTTTA	170	0.0	18.5	26
TGGACCC	2195	0.0	18.120728	5
ACGCGAA	105	4.803096E-7	17.619047	34
GGACCCT	2220	0.0	17.583334	6
TGACGCG	85	2.7252863E-5	17.411764	32
GTATCAA	19405	0.0	17.389332	2
CATTGCG	120	1.0426811E-7	16.958334	29
TCCGGTC	295	0.0	16.932203	8
GACCCTC	2305	0.0	16.373102	7
TCACGTA	285	0.0	16.22807	25
TAGACTA	175	1.3278623E-10	15.857143	5
TACCCGG	105	9.352221E-6	15.857142	20
GTCGTCA	130	2.5924965E-7	15.653846	24
>>END_MODULE