FastQCFastQC Report
Wed 25 May 2016
SRR2088305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556882
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT562191.5805697237074492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT549081.543711599091564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT494811.3911341450180243No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG189890.5338664594439737No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA186060.5230986015279675No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185070.5203152648864933No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG180740.508141681393985No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA176540.4963335865513672No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG172930.4861842478890219No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA141150.39683633024654735No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA103640.2913787974973586No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA101640.28575589519135014No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC89070.250415954198087No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA88760.24954440434065564No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA77150.21690345645427653No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA74790.21026843173318652No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG71090.19986606246707087No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.19278120556150022No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG67010.1883953417628136No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG65340.18370021833729655No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58710.16506029719287849No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC55040.15474227146135294No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT52720.14821970478638313No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC52020.14625168897928015No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG50760.14270926052649485No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT46060.12949544010737493No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT46060.12949544010737493No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45390.1276117678348621No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA45140.12690890504661104No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG44200.12426614096278707No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA42040.11819340647229792No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT41890.1177716887993473No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA41420.11645030675743531No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT40460.11375131365055123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40160.11290787830464998No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA40100.11273919123546972No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG38540.10835332743678312No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT38250.10753800660241188No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37460.10531696019153855No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA37400.10514827312235828No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG37240.1046984409378776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC36620.10295534122301499No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35970.10112789797356223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128050.032.1889881
GTATTGG11900.025.9621851
ATTGGAC14300.022.1223773
TTGGACC24150.020.0703954
GGACCCT22600.019.9734526
TGGACCC24000.019.193755
GACCCTC24000.018.268757
GTATCAA233800.017.7324642
TATTGGA17750.017.6140862
GACGCTT752.068801E-417.26666614
CGAATTA1801.0913936E-1116.44444515
TACCCCG2600.016.3653855
GTGGTAT14050.016.195731
GATACCT17500.015.43428636
CAATGCG1701.4897523E-915.23529419
TATACCG1601.0986696E-815.031255
TTAACGG3100.014.91935535
TTTTTAC30550.014.715221
GTATAGA1904.5656634E-1014.6052641
GATACTG15050.014.50498236
Produced by FastQC (version 0.11.5)