##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3556882
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953003501381264	31.0	31.0	33.0	28.0	34.0
2	31.258810103905613	31.0	31.0	34.0	28.0	34.0
3	31.305249935196052	31.0	31.0	34.0	28.0	34.0
4	35.198965835807876	35.0	35.0	37.0	33.0	37.0
5	34.976726526210314	35.0	35.0	37.0	32.0	37.0
6	35.03973367685518	36.0	35.0	37.0	32.0	37.0
7	34.847638746520126	35.0	35.0	37.0	32.0	37.0
8	34.92350688046441	36.0	35.0	37.0	32.0	37.0
9	36.37998421089033	38.0	35.0	39.0	32.0	39.0
10	36.20486847750361	38.0	35.0	39.0	32.0	39.0
11	36.29650547867486	38.0	35.0	39.0	32.0	39.0
12	36.04401326780028	38.0	35.0	39.0	30.0	39.0
13	36.13934086090008	38.0	35.0	39.0	31.0	39.0
14	36.93415412712595	39.0	36.0	40.0	31.0	41.0
15	37.04878458155204	39.0	36.0	40.0	31.0	41.0
16	37.00425709933588	38.0	36.0	40.0	31.0	41.0
17	37.13067906104279	39.0	36.0	40.0	31.0	41.0
18	37.14678586469835	39.0	36.0	40.0	31.0	41.0
19	37.241515743283024	39.0	36.0	40.0	31.0	41.0
20	37.23050272682647	39.0	36.0	40.0	31.0	41.0
21	37.1612277269811	39.0	36.0	40.0	31.0	41.0
22	37.094451544920524	39.0	36.0	40.0	31.0	41.0
23	36.98670492864256	39.0	36.0	40.0	31.0	41.0
24	36.894475273568254	39.0	35.0	40.0	31.0	41.0
25	36.75758459234802	38.0	35.0	40.0	30.0	41.0
26	36.515708139882065	38.0	35.0	40.0	30.0	41.0
27	36.3942739736657	38.0	35.0	40.0	30.0	41.0
28	36.214551958709905	38.0	35.0	40.0	30.0	41.0
29	35.995600922380895	38.0	35.0	40.0	29.0	41.0
30	35.74337776738166	38.0	34.0	40.0	29.0	41.0
31	35.45934107457037	38.0	34.0	40.0	27.0	41.0
32	35.34970431968224	37.0	34.0	40.0	27.0	41.0
33	35.146451302011144	37.0	33.0	40.0	27.0	41.0
34	35.26533042141966	38.0	34.0	40.0	27.0	41.0
35	35.14102267097981	38.0	33.0	40.0	26.0	41.0
36	35.04718627157156	38.0	33.0	40.0	25.0	41.0
37	34.9091890031775	38.0	33.0	40.0	25.0	41.0
38	34.827353845306085	38.0	33.0	40.0	24.0	41.0
39	34.683335291977635	38.0	33.0	40.0	24.0	41.0
40	34.449573249829484	38.0	33.0	40.0	23.0	41.0
41	34.29170605041157	38.0	33.0	40.0	22.0	41.0
42	34.082514685615095	38.0	33.0	40.0	21.0	41.0
43	33.69191640318684	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	10.0
16	44.0
17	128.0
18	272.0
19	728.0
20	1571.0
21	2915.0
22	5428.0
23	9425.0
24	15787.0
25	24445.0
26	37280.0
27	53570.0
28	74536.0
29	99149.0
30	126482.0
31	152430.0
32	182724.0
33	221165.0
34	263203.0
35	312581.0
36	383713.0
37	489517.0
38	565490.0
39	534285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75558312027219	22.257527801034726	14.83358177190022	22.15330730679286
2	18.35655498270676	23.919910753294598	36.311662855276055	21.411871408722583
3	19.657947606920892	24.872683434536203	34.220393029625384	21.248975928917517
4	12.80551336816909	16.129435837342932	36.48456710118582	34.58048369330216
5	11.534315729338223	39.00770393844946	35.56128654253922	13.89669378967309
6	31.4014071875311	37.11973014567253	12.850131097967266	18.628731568829103
7	24.287789136665204	35.03979046816847	21.147960488990076	19.524459906176254
8	26.60535266562118	34.258431963725535	19.02672059404838	20.1094947766049
9	23.54927714779405	14.532897071086417	23.672699853410936	38.24512592770859
10	15.859339725073815	27.558940667697158	34.23245415507177	22.349265452157255
11	31.46637982367703	22.17357786960602	25.476611256713046	20.88343105000391
12	21.642044914619042	27.315581455893113	31.518644700611375	19.523728928876473
13	32.28569291868552	21.093896283317804	28.136187818431985	18.484222979564684
14	19.886068753475655	23.70773053477737	29.26186474558335	27.144335966163624
15	20.982562817658838	29.684960029598955	28.05679243787115	21.27568471487106
16	20.45381319931333	29.239120105755546	28.39855244003034	21.908514254900783
17	20.716993141746055	26.52590105603728	27.55503275059448	25.202073051622182
18	23.063936335250933	24.679143137163393	31.952226697427694	20.304693830157987
19	21.9544814812524	23.764212588441225	33.46400021142112	20.817305718885248
20	23.42307110553569	22.683322078157218	33.907647203365194	19.985959612941897
21	21.818210443866285	24.018424001695866	34.85648385299259	19.306881701445256
22	19.299965531608866	24.521280154922202	34.08445936637763	22.0942949470913
23	21.459244360650708	24.7320265333514	35.6323881421987	18.17634096379919
24	21.1093311501478	24.5580539360035	31.967211732073203	22.3654031817755
25	20.81736194790831	25.605010230870747	33.37206575871789	20.205562062503056
26	20.348327552052613	26.6146304544261	33.1897712659571	19.847270727564197
27	18.472358655699008	26.843313891211462	32.78053643612579	21.903791016963734
28	19.732816551125396	25.63289982630855	32.806542359291086	21.82774126327497
29	19.990963995994242	25.59975281721463	31.523171136967715	22.886112049823414
30	20.52511160055352	25.783003203367443	34.33892381023605	19.352961385842992
31	22.043632597314165	25.414534415254707	33.5240528080493	19.01778017938183
32	21.195361555429727	25.543214534527714	31.838109895127246	21.42331401491531
33	20.48074690135911	25.492917673400466	32.97812522315893	21.048210202081485
34	19.140359449652813	26.652950533641544	34.03177277177033	20.174917244935312
35	18.403084499288987	30.030571719837766	31.817417614641137	19.74892616623211
36	22.147909320579092	26.42013426366126	31.42699139302344	20.004965022736204
37	20.95096210669907	27.83977652337075	31.075306968294143	20.13395440163604
38	20.697003724048198	25.466967979258236	32.86763519284587	20.968393103847696
39	20.077331775414535	25.085988233514634	33.70848400368637	21.12819598738446
40	17.98788939301332	26.182060580024864	33.064605460625344	22.76544456633647
41	20.857818730000037	24.91007573487116	32.98723432489467	21.24487121023413
42	18.83973097786207	28.133910542998052	31.995438701649366	21.03091977749051
43	19.514282453002377	28.425261225983885	30.30522238297475	21.75523393803899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	337.0
2	422.0
3	1349.5
4	2277.0
5	2277.0
6	4157.5
7	6038.0
8	6975.5
9	7913.0
10	12280.5
11	16648.0
12	16648.0
13	31976.5
14	47305.0
15	80643.5
16	113982.0
17	110772.0
18	107562.0
19	107562.0
20	112154.0
21	116746.0
22	92076.0
23	67406.0
24	71967.5
25	76529.0
26	76529.0
27	80750.0
28	84971.0
29	87383.5
30	89796.0
31	96906.0
32	104016.0
33	104016.0
34	114048.5
35	124081.0
36	135731.5
37	147382.0
38	154854.0
39	162326.0
40	162326.0
41	167416.5
42	172507.0
43	180328.0
44	188149.0
45	211897.5
46	235646.0
47	235646.0
48	350193.5
49	464741.0
50	419622.5
51	374504.0
52	325520.0
53	276536.0
54	276536.0
55	231443.0
56	186350.0
57	147133.5
58	107917.0
59	93623.0
60	79329.0
61	79329.0
62	70246.0
63	61163.0
64	52803.0
65	44443.0
66	38006.5
67	31570.0
68	31570.0
69	26803.5
70	22037.0
71	18583.5
72	15130.0
73	12320.0
74	9510.0
75	9510.0
76	7574.5
77	5639.0
78	4212.0
79	2785.0
80	2177.0
81	1569.0
82	1569.0
83	1196.5
84	824.0
85	670.0
86	516.0
87	364.0
88	212.0
89	212.0
90	163.5
91	115.0
92	71.0
93	27.0
94	18.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3556882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.124780738716545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13270687546182	24.27388025476529
2	13.922238549095125	9.501866757599567
3	5.51799961208415	5.649015806360837
4	2.8169395255649694	3.8450977465651515
5	1.6157428075973108	2.756843451970825
6	0.9971042426547293	2.0415578192541997
7	0.6833360722727109	1.6323085546013416
8	0.4936545563914288	1.3476682798020714
9	0.37359520257858514	1.1473968935727548
>10	2.086922377910252	13.475878939963726
>50	0.19724365523334447	4.707593760061488
>100	0.14019154223199876	9.11716262156034
>500	0.01120398670892795	2.60884617002978
>1k	0.009046181861237257	6.807860221693167
>5k	0.0010789024238172875	2.4668446426526938
>10k+	9.959099296774961E-4	8.620178079546871
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	56219	1.5805697237074492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54908	1.543711599091564	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	49481	1.3911341450180243	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18989	0.5338664594439737	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	18606	0.5230986015279675	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18507	0.5203152648864933	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	18074	0.508141681393985	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	17654	0.4963335865513672	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	17293	0.4861842478890219	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	14115	0.39683633024654735	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10364	0.2913787974973586	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	10164	0.28575589519135014	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8907	0.250415954198087	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8876	0.24954440434065564	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7715	0.21690345645427653	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	7479	0.21026843173318652	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	7109	0.19986606246707087	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6857	0.19278120556150022	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	6701	0.1883953417628136	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	6534	0.18370021833729655	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5871	0.16506029719287849	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	5504	0.15474227146135294	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5272	0.14821970478638313	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5202	0.14625168897928015	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	5076	0.14270926052649485	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4606	0.12949544010737493	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4606	0.12949544010737493	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4539	0.1276117678348621	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	4514	0.12690890504661104	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4420	0.12426614096278707	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	4204	0.11819340647229792	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	4189	0.1177716887993473	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	4142	0.11645030675743531	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	4046	0.11375131365055123	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4016	0.11290787830464998	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4010	0.11273919123546972	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3854	0.10835332743678312	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3825	0.10753800660241188	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3746	0.10531696019153855	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3740	0.10514827312235828	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3724	0.1046984409378776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3662	0.10295534122301499	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3597	0.10112789797356223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8114511530042322E-5	0.0
3	0.0	0.0	0.0	2.8114511530042322E-5	0.0
4	0.0	0.0	0.0	2.8114511530042322E-5	0.0
5	0.0	0.0	0.0	2.8114511530042322E-5	0.0
6	2.8114511530042322E-5	0.0	0.0	2.8114511530042322E-5	0.0
7	2.8114511530042322E-5	0.0	0.0	5.6229023060084644E-5	0.0
8	2.8114511530042322E-5	0.0	0.0	5.6229023060084644E-5	0.0
9	5.6229023060084644E-5	0.0	0.0	1.1245804612016929E-4	0.0
10	5.6229023060084644E-5	0.0	0.0	2.2491609224033858E-4	2.8114511530042322E-5
11	5.6229023060084644E-5	0.0	0.0	5.341757190708041E-4	2.8114511530042322E-5
12	5.6229023060084644E-5	0.0	0.0	0.0010683514381416082	2.8114511530042322E-5
13	5.6229023060084644E-5	0.0	0.0	0.0013213820419119891	2.8114511530042322E-5
14	5.6229023060084644E-5	0.0	0.0	0.001546298134152328	2.8114511530042322E-5
15	5.6229023060084644E-5	0.0	0.0	0.002108588364753174	2.8114511530042322E-5
16	1.1245804612016929E-4	0.0	0.0	0.0029239091991244016	2.8114511530042322E-5
17	1.1245804612016929E-4	0.0	0.0	0.004048489660326095	2.8114511530042322E-5
18	1.6868706918025394E-4	0.0	0.0	0.004976268540817491	2.8114511530042322E-5
19	1.6868706918025394E-4	0.0	0.0	0.006691253744150073	2.8114511530042322E-5
20	1.6868706918025394E-4	0.0	0.0	0.010093109639285194	2.8114511530042322E-5
21	1.9680158071029626E-4	0.0	0.0	0.018836722725128357	2.8114511530042322E-5
22	1.9680158071029626E-4	0.0	0.0	0.030532359521625963	2.8114511530042322E-5
23	1.9680158071029626E-4	0.0	0.0	0.03874179688839832	2.8114511530042322E-5
24	1.9680158071029626E-4	0.0	0.0	0.047625982531891695	2.8114511530042322E-5
25	1.9680158071029626E-4	0.0	0.0	0.05285528167647957	2.8114511530042322E-5
26	1.9680158071029626E-4	0.0	0.0	0.06277970424658451	2.8114511530042322E-5
27	2.2491609224033858E-4	0.0	0.0	0.1450146504719583	2.8114511530042322E-5
28	2.2491609224033858E-4	0.0	0.0	0.25064087029032733	2.8114511530042322E-5
29	2.2491609224033858E-4	0.0	0.0	0.3704649184313677	2.8114511530042322E-5
30	2.2491609224033858E-4	0.0	0.0	0.579299510076522	2.8114511530042322E-5
31	2.2491609224033858E-4	0.0	0.0	0.8525163331254734	2.8114511530042322E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12805	0.0	32.188988	1
GTATTGG	1190	0.0	25.962185	1
ATTGGAC	1430	0.0	22.122377	3
TTGGACC	2415	0.0	20.070395	4
GGACCCT	2260	0.0	19.973452	6
TGGACCC	2400	0.0	19.19375	5
GACCCTC	2400	0.0	18.26875	7
GTATCAA	23380	0.0	17.732464	2
TATTGGA	1775	0.0	17.614086	2
GACGCTT	75	2.068801E-4	17.266666	14
CGAATTA	180	1.0913936E-11	16.444445	15
TACCCCG	260	0.0	16.365385	5
GTGGTAT	1405	0.0	16.19573	1
GATACCT	1750	0.0	15.434286	36
CAATGCG	170	1.4897523E-9	15.235294	19
TATACCG	160	1.0986696E-8	15.03125	5
TTAACGG	310	0.0	14.919355	35
TTTTTAC	3055	0.0	14.71522	1
GTATAGA	190	4.5656634E-10	14.605264	1
GATACTG	1505	0.0	14.504982	36
>>END_MODULE