FastQCFastQC Report
Wed 25 May 2016
SRR2088304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844220
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120511.4274715121650754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113701.3468053351022244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87541.036933500746251No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.4734547866669826No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA31300.37075643789533536No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30130.3568974911752861No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29790.35287010494894694No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28380.33616829736324655No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27150.32159863542678446No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23580.27931108005022387No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14700.17412522802113195No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.17069010447513683No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA14130.1673734334652105No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13190.15623889507474356No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12350.14628888204496457No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12250.1451043566842766No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12050.14273530596290068No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.1282840965625074No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10730.12709957120181942No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9920.11750491578024687No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG9780.11584658027528369No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT9670.11454360237852693No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC9640.11418824477032054No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9410.11146383644073819No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8480.10044775058634006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.030.4543761
AATTACG400.001930764523.12500216
ATTGGAC2700.019.870373
TTGGACC4150.018.722894
GTATTGG3200.018.51
CGGCATA500.007033370418.536
ATTCCGT609.234044E-418.56
GTATCAA45100.018.08982
TGGACCC4650.017.9032255
GGACCCT4300.017.6395346
TGCGGAC700.002591728715.85714322
TATACTG1059.337144E-615.8571425
GTGGTAT3850.015.3766231
TAGGTTA1353.9681981E-715.07407533
AGGTTAC1353.9681981E-715.07407534
GACCCTC4950.014.9494957
AGGTAAT750.00410320614.81
AGTGCGG750.00410320614.820
TAGGACA1001.09281704E-414.84
ATACTGC1252.956309E-614.7999996
Produced by FastQC (version 0.11.5)