##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844220
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.590329534955345	31.0	30.0	31.0	27.0	34.0
2	30.8799234796617	31.0	31.0	34.0	27.0	34.0
3	30.886995096065007	31.0	30.0	34.0	27.0	34.0
4	34.787860984103666	35.0	35.0	37.0	32.0	37.0
5	34.52082277131554	35.0	35.0	37.0	30.0	37.0
6	34.64802184264765	35.0	35.0	37.0	31.0	37.0
7	34.45990263201535	35.0	35.0	37.0	30.0	37.0
8	34.55870389235034	35.0	35.0	37.0	30.0	37.0
9	35.97781976262112	37.0	35.0	39.0	30.0	39.0
10	35.70980905451186	37.0	35.0	39.0	30.0	39.0
11	35.85227665774324	37.0	35.0	39.0	30.0	39.0
12	35.57983464025965	37.0	35.0	39.0	30.0	39.0
13	35.690588946009335	37.0	35.0	39.0	30.0	39.0
14	36.435125915045845	38.0	35.0	40.0	30.0	41.0
15	36.54534363080714	38.0	35.0	40.0	30.0	41.0
16	36.47005638340717	38.0	35.0	40.0	30.0	41.0
17	36.57315865532681	38.0	35.0	40.0	30.0	41.0
18	36.57641136196726	38.0	35.0	40.0	30.0	41.0
19	36.67374262632963	38.0	35.0	40.0	30.0	41.0
20	36.64575229205657	38.0	35.0	40.0	30.0	41.0
21	36.56107768117315	38.0	35.0	40.0	30.0	41.0
22	36.48209353012248	38.0	35.0	40.0	30.0	41.0
23	36.347602520669966	38.0	35.0	40.0	30.0	41.0
24	36.26506597806259	38.0	35.0	40.0	30.0	41.0
25	36.12464523465447	38.0	34.0	40.0	29.0	41.0
26	35.83490085522731	38.0	34.0	40.0	29.0	41.0
27	35.67634976664851	38.0	34.0	40.0	27.0	41.0
28	35.483578924924785	38.0	34.0	40.0	27.0	41.0
29	35.28209116107176	38.0	34.0	40.0	27.0	41.0
30	35.05350856411836	37.0	33.0	40.0	27.0	41.0
31	34.77069484257658	37.0	33.0	40.0	25.0	41.0
32	34.651127668143374	37.0	33.0	40.0	25.0	41.0
33	34.465815782615906	37.0	33.0	40.0	25.0	41.0
34	34.61448200705977	37.0	33.0	40.0	25.0	41.0
35	34.526112861576365	37.0	33.0	40.0	25.0	41.0
36	34.46056478169198	37.0	33.0	40.0	24.0	41.0
37	34.337462983582476	37.0	33.0	40.0	24.0	41.0
38	34.286561559782996	37.0	33.0	40.0	24.0	41.0
39	34.15899054748762	37.0	33.0	40.0	23.0	41.0
40	33.962872237094594	37.0	33.0	40.0	22.0	41.0
41	33.820834616569144	37.0	33.0	40.0	21.0	41.0
42	33.64252564497406	37.0	32.0	40.0	21.0	41.0
43	33.238476937291225	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	23.0
17	55.0
18	124.0
19	256.0
20	577.0
21	1141.0
22	1961.0
23	3208.0
24	5204.0
25	7874.0
26	11182.0
27	15924.0
28	21344.0
29	27576.0
30	34295.0
31	41211.0
32	48888.0
33	58110.0
34	67997.0
35	79679.0
36	95990.0
37	117628.0
38	130747.0
39	73219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.606619127715526	24.150813768922795	14.818056904598329	21.424510198763354
2	20.60943829807396	25.137760299448008	33.461775366610595	20.79102603586743
3	21.753808249034613	25.614887114733126	32.42353888796759	20.20776574826467
4	15.483760157304967	17.804363791428774	35.06100305607543	31.650872995190827
5	12.009665726943213	39.84447182014167	35.087299519082706	13.058562933832413
6	31.89630664992538	37.79263699035796	13.221198265854872	17.08985809386179
7	24.37859799578309	34.697590675416365	21.912771552438937	19.011039776361613
8	26.526024022174315	35.34351235459951	19.36083011537277	18.769633507853403
9	24.152116746819548	14.414015304067659	22.812063206273248	38.621804742839544
10	16.215796830210135	28.186254767714576	34.08696785198171	21.510980550093578
11	32.53215986354268	22.564260500817323	24.237047215180876	20.66653242045912
12	20.358792731752388	27.84712515694961	31.5974509014238	20.1966312098742
13	31.637606311151124	21.22799744142522	27.668143374949654	19.466252872473998
14	19.975243419961622	23.474212882897824	28.740138826372274	27.810404870768284
15	21.95991566179432	29.936035630522852	26.875695908649405	21.228352799033427
16	20.8579517187463	28.918172988083672	28.172751178602734	22.051124114567294
17	20.847409443036174	27.328658406576483	28.169434507592804	23.654497642794535
18	22.3145625547843	25.321598635426785	31.225983748312053	21.137855061476866
19	21.955532917959776	24.577124446234393	32.673592191608826	20.79375044419701
20	22.829949539219633	24.273649048826133	32.80827272511904	20.088128686835187
21	21.935751344436287	24.961858283385848	33.32756864324465	19.77482172893322
22	20.10814716543081	25.39468385018123	32.77522446755585	21.721944516832107
23	21.5215228258037	25.702068181279763	33.87967591386132	18.896733079055224
24	21.34514699959726	25.544526308308264	31.282959418161145	21.827367273933334
25	21.25535997725711	26.18689441140935	32.17514391983132	20.382601691502213
26	21.04581744095141	26.858994101063704	32.0051645305726	20.090023927412286
27	19.751249674255526	26.92319537561299	31.7754850631352	21.550069886996283
28	20.502949468148113	26.45045130416242	32.07576224206961	20.97083698561986
29	20.64023595745185	25.883892824145367	31.23818435952714	22.237686858875648
30	20.936248845087775	26.291251095685958	32.822605481983366	19.9498945772429
31	21.840397052900904	26.07566748004075	32.443794271635355	19.640141195422995
32	21.22550993816778	26.105399066594014	31.369429769491365	21.299661225746842
33	20.951647674776716	26.255241524721047	31.801544621070338	20.9915661794319
34	20.06585961005425	26.967496624102722	32.615787354007246	20.350856411835778
35	19.53720594157921	28.959749828243826	31.452228092203455	20.050816137973516
36	22.14707066878302	26.268034398616475	30.934353604510672	20.650541328089833
37	21.214256947241243	27.28755537656061	30.922152993295587	20.57603468290256
38	21.09722584160527	25.535168557958826	32.1771576129445	21.19044798749141
39	20.53291795977352	25.522849494207673	32.78552983819383	21.158702707824975
40	19.166094146075668	26.326786856506594	32.05988960223639	22.44722939518135
41	20.9118476226576	25.32775816730236	32.099334296747294	21.661059913292743
42	19.64653763237071	27.500177678804107	31.27916893700694	21.574115751818248
43	20.06976854374452	27.626211177181304	30.223401483025754	22.080618796048423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	106.5
2	124.0
3	282.0
4	440.0
5	440.0
6	870.5
7	1301.0
8	1486.0
9	1671.0
10	2537.5
11	3404.0
12	3404.0
13	6760.0
14	10116.0
15	17119.5
16	24123.0
17	22900.5
18	21678.0
19	21678.0
20	23973.5
21	26269.0
22	21450.0
23	16631.0
24	17803.0
25	18975.0
26	18975.0
27	20220.5
28	21466.0
29	22645.0
30	23824.0
31	25778.5
32	27733.0
33	27733.0
34	30562.5
35	33392.0
36	36006.0
37	38620.0
38	40550.0
39	42480.0
40	42480.0
41	44010.0
42	45540.0
43	47229.0
44	48918.0
45	52034.5
46	55151.0
47	55151.0
48	73563.5
49	91976.0
50	83945.0
51	75914.0
52	67471.0
53	59028.0
54	59028.0
55	51001.5
56	42975.0
57	36152.5
58	29330.0
59	26144.5
60	22959.0
61	22959.0
62	20627.0
63	18295.0
64	15981.0
65	13667.0
66	11624.0
67	9581.0
68	9581.0
69	8193.5
70	6806.0
71	5796.5
72	4787.0
73	3935.5
74	3084.0
75	3084.0
76	2461.0
77	1838.0
78	1414.0
79	990.0
80	752.0
81	514.0
82	514.0
83	389.5
84	265.0
85	205.5
86	146.0
87	107.5
88	69.0
89	69.0
90	54.5
91	40.0
92	22.5
93	5.0
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	844220.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.1269804516918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.49321345493672	46.02728745979227
2	9.50269734402314	10.477100214446144
3	2.7322544409325014	4.518628114630023
4	1.2398716783881447	2.7340152710843824
5	0.6572115746162921	1.8115044813248957
6	0.4132652990197848	1.3669240836255745
7	0.30486419733712505	1.1764369852917083
8	0.2270544333753763	1.0013460248123462
9	0.16894927768221082	0.8382297175303217
>10	1.113237956202103	11.49359349761771
>50	0.09114355811931125	3.5047655326417613
>100	0.04503252162000731	4.6464452833436996
>500	0.007110398147300715	2.7223242349262264
>1k	0.0034474657683882253	3.8596449971505216
>5k	2.1546661052426408E-4	1.0398021882518516
>10k+	4.3093322104852816E-4	2.781951913530571
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12051	1.4274715121650754	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11370	1.3468053351022244	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8754	1.036933500746251	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3997	0.4734547866669826	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3130	0.37075643789533536	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3013	0.3568974911752861	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2979	0.35287010494894694	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2838	0.33616829736324655	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2715	0.32159863542678446	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2358	0.27931108005022387	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1470	0.17412522802113195	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.17069010447513683	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1413	0.1673734334652105	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1319	0.15623889507474356	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1235	0.14628888204496457	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1225	0.1451043566842766	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1205	0.14273530596290068	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1083	0.1282840965625074	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1073	0.12709957120181942	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	992	0.11750491578024687	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	978	0.11584658027528369	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	967	0.11454360237852693	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	964	0.11418824477032054	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	941	0.11146383644073819	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	848	0.10044775058634006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.1845253606879723E-4	0.0	0.0	0.0	0.0
3	1.1845253606879723E-4	0.0	0.0	0.0	0.0
4	1.1845253606879723E-4	0.0	0.0	0.0	0.0
5	2.3690507213759446E-4	0.0	0.0	0.0	0.0
6	2.3690507213759446E-4	0.0	0.0	0.0	0.0
7	2.3690507213759446E-4	0.0	0.0	0.0	0.0
8	2.3690507213759446E-4	0.0	0.0	0.0	0.0
9	2.3690507213759446E-4	0.0	0.0	0.0	0.0
10	2.3690507213759446E-4	0.0	0.0	2.3690507213759446E-4	0.0
11	2.3690507213759446E-4	0.0	0.0	5.922626803439862E-4	0.0
12	2.3690507213759446E-4	0.0	0.0	0.0010660728246191752	0.0
13	2.3690507213759446E-4	0.0	0.0	0.0010660728246191752	0.0
14	2.3690507213759446E-4	0.0	0.0	0.0014214304328255668	0.0
15	2.3690507213759446E-4	0.0	0.0	0.002013693113169553	0.0
16	2.3690507213759446E-4	0.0	0.0	0.0026059557935135392	0.0
17	2.3690507213759446E-4	0.0	0.0	0.003553576082063917	0.0
18	2.3690507213759446E-4	0.0	0.0	0.004027386226339106	0.0
19	2.3690507213759446E-4	0.0	0.0	0.004856553978820686	0.0
20	3.553576082063917E-4	0.0	0.0	0.007936319916609414	0.0
21	3.553576082063917E-4	0.0	0.0	0.013148231503636493	0.0
22	3.553576082063917E-4	0.0	0.0	0.02475658003837862	0.0
23	3.553576082063917E-4	0.0	0.0	0.030916111913956077	0.0
24	3.553576082063917E-4	0.0	0.0	0.038260169150221505	0.0
25	3.553576082063917E-4	0.0	0.0	0.043353628201179786	0.0
26	3.553576082063917E-4	0.0	0.0	0.05105304304565161	0.0
27	3.553576082063917E-4	0.0	0.0	0.11229300419321978	0.0
28	3.553576082063917E-4	0.0	0.0	0.1920115609675203	0.0
29	3.553576082063917E-4	0.0	0.0	0.28049560541091184	0.0
30	3.553576082063917E-4	0.0	0.0	0.44324938996943924	0.0
31	3.553576082063917E-4	0.0	0.0	0.6613205088720949	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2685	0.0	30.454376	1
AATTACG	40	0.0019307645	23.125002	16
ATTGGAC	270	0.0	19.87037	3
TTGGACC	415	0.0	18.72289	4
GTATTGG	320	0.0	18.5	1
CGGCATA	50	0.0070333704	18.5	36
ATTCCGT	60	9.234044E-4	18.5	6
GTATCAA	4510	0.0	18.0898	2
TGGACCC	465	0.0	17.903225	5
GGACCCT	430	0.0	17.639534	6
TGCGGAC	70	0.0025917287	15.857143	22
TATACTG	105	9.337144E-6	15.857142	5
GTGGTAT	385	0.0	15.376623	1
TAGGTTA	135	3.9681981E-7	15.074075	33
AGGTTAC	135	3.9681981E-7	15.074075	34
GACCCTC	495	0.0	14.949495	7
AGGTAAT	75	0.004103206	14.8	1
AGTGCGG	75	0.004103206	14.8	20
TAGGACA	100	1.09281704E-4	14.8	4
ATACTGC	125	2.956309E-6	14.799999	6
>>END_MODULE