FastQCFastQC Report
Wed 25 May 2016
SRR2088303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504717
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT394191.573790571948847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT387541.5472406663108047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT324251.2945574290428818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133640.5335532916493161No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG110460.4410079062824263No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA105720.4220836126396715No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA104090.41557589140809126No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100190.4000052700564575No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG97840.39062297257534484No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87080.34766402751288866No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA64720.25839246509685526No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55150.22018455578015403No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53350.21299811515632308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50170.2003020700542217No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47230.1885642170352978No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45730.18257551651543868No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42490.16963992339254294No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38250.1527118632564078No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG37890.1512745751316416No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37710.15055593106925852No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.14560527197284165No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36410.14536572395204728No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32770.1308331440238558No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG32640.13031412331213468No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT32020.12783879376392623No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT31970.12763917041326425No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT30900.12336723070909808No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30460.12161054522327272No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.11194877505123332No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG27940.1115495283499094No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA27780.1109107336277911No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT27710.11063126093686432No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA27470.10967306885368686No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT25750.10280602559091506No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA25650.10240677888959113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC25060.10005122335177986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96150.029.8616751
TCACGTT1250.025.1624
GTATTGG8500.022.4176451
ATTGGAC8650.021.8150313
CGCGATA855.1868483E-821.76470614
TTGGACC14800.019.54
TGGACCC15000.019.3633355
GGACCCT15350.018.680786
GACCCTC15250.018.4393447
ACGTGCG1054.8028414E-717.6190479
TATTGGA10900.017.3119262
GTCCCGA1107.816652E-716.81818211
GTATCAA171850.016.739892
GTGCGCG1151.2435503E-616.08695611
TGCGCGA1059.351823E-615.85714212
GCGATAT1059.351823E-615.85714215
TACTACG1059.351823E-615.8571425
TTTTTAC19550.015.61381151
GCGGACT1201.937622E-615.41666637
ACGTTTA2055.456968E-1215.34146326
Produced by FastQC (version 0.11.5)