##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504717
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.832151895802998	31.0	31.0	33.0	28.0	34.0
2	31.13055606681314	31.0	31.0	34.0	28.0	34.0
3	31.168561158805566	31.0	31.0	34.0	28.0	34.0
4	35.07618465479334	35.0	35.0	37.0	32.0	37.0
5	34.84026818199421	35.0	35.0	37.0	32.0	37.0
6	34.903476520501115	35.0	35.0	37.0	32.0	37.0
7	34.70747713214706	35.0	35.0	37.0	31.0	37.0
8	34.786335142852465	35.0	35.0	37.0	32.0	37.0
9	36.21947908685892	38.0	35.0	39.0	32.0	39.0
10	36.026488022399334	38.0	35.0	39.0	30.0	39.0
11	36.13269842461244	38.0	35.0	39.0	31.0	39.0
12	35.87492598964274	37.0	35.0	39.0	30.0	39.0
13	35.96513218858657	37.0	35.0	39.0	30.0	39.0
14	36.73133571577148	38.0	36.0	40.0	31.0	41.0
15	36.86183429105963	38.0	36.0	40.0	31.0	41.0
16	36.82371820848423	38.0	36.0	40.0	31.0	41.0
17	36.94573997780987	38.0	36.0	40.0	31.0	41.0
18	36.96172621497758	38.0	36.0	40.0	31.0	41.0
19	37.068223276322236	39.0	36.0	40.0	31.0	41.0
20	37.056941762282925	39.0	36.0	40.0	31.0	41.0
21	36.98139909618532	39.0	36.0	40.0	31.0	41.0
22	36.904615172093294	39.0	36.0	40.0	31.0	41.0
23	36.78585205434386	39.0	35.0	40.0	30.0	41.0
24	36.69418261623968	38.0	35.0	40.0	30.0	41.0
25	36.567740786683686	38.0	35.0	40.0	30.0	41.0
26	36.30061879246238	38.0	35.0	40.0	30.0	41.0
27	36.159992925348455	38.0	35.0	40.0	30.0	41.0
28	35.974175525618264	38.0	34.0	40.0	29.0	41.0
29	35.7530830828393	38.0	34.0	40.0	29.0	41.0
30	35.5116861505711	38.0	34.0	40.0	27.0	41.0
31	35.21457434113315	37.0	34.0	40.0	27.0	41.0
32	35.08437400313089	37.0	33.0	40.0	27.0	41.0
33	34.87851960920136	37.0	33.0	40.0	26.0	41.0
34	34.99281236163606	37.0	33.0	40.0	26.0	41.0
35	34.89425671642744	37.0	33.0	40.0	25.0	41.0
36	34.79909905989379	37.0	33.0	40.0	25.0	41.0
37	34.654934270019325	37.0	33.0	40.0	24.0	41.0
38	34.57349193541626	37.0	33.0	40.0	24.0	41.0
39	34.42510071996158	37.0	33.0	40.0	23.0	41.0
40	34.1948363827131	37.0	33.0	40.0	22.0	41.0
41	34.02249076442568	37.0	33.0	40.0	21.0	41.0
42	33.814955142636876	37.0	33.0	40.0	20.0	41.0
43	33.415056072202965	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	6.0
15	10.0
16	37.0
17	119.0
18	280.0
19	612.0
20	1323.0
21	2554.0
22	4584.0
23	7767.0
24	12678.0
25	19616.0
26	29114.0
27	41567.0
28	57052.0
29	75089.0
30	93996.0
31	113600.0
32	135662.0
33	161802.0
34	190557.0
35	223446.0
36	273197.0
37	340571.0
38	391858.0
39	327617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.89979706290171	23.11414822512883	15.318177662386608	21.66787704958285
2	18.434298166219975	24.73620772326774	35.80184907117251	21.027645039339774
3	19.840524897623165	25.549113931833418	34.097065656519284	20.513295514024136
4	13.3274936849153	16.889652603467777	36.41557110044767	33.367282611169244
5	11.49315471568245	39.51687955166193	35.32482911243066	13.66513662022496
6	30.885445341729223	37.74186864224581	13.342625134895481	18.030060881129483
7	23.5192239282921	35.26482233322168	21.95026424142927	19.265689497056954
8	26.29937833296137	34.76871838215655	19.507752772069658	19.424150512812425
9	23.466643137727736	14.71671250684209	23.926774961003577	37.8898693944266
10	15.901437168350757	28.228378695078128	34.122537596063744	21.747646540507372
11	31.341385074641163	22.878712445358097	25.08363220275983	20.696270277240902
12	20.771408506430067	28.018055532820675	31.99447282866687	19.216063132082386
13	31.724981305273207	21.594096259178183	28.140264948095933	18.540657487452673
14	19.513821321929782	24.058446523100212	29.795302223764203	26.632429931205802
15	20.87752827964197	30.34710108966402	27.878997906749547	20.896372723944463
16	20.143712842608565	29.29508603167543	28.759815979210423	21.801385146505574
17	20.255382144968873	27.187422770716214	28.42868076513235	24.128514319182568
18	22.323240509806098	25.2990257981241	32.20463629224379	20.17309739982601
19	21.478234866453974	24.607051415389446	33.78445548938263	20.13025822877395
20	22.611975724203575	23.890603209863627	33.978289762875406	19.519131303057392
21	21.339336939063376	24.978590395641504	34.71893231850145	18.96314034679367
22	19.104473679062345	25.414048772775526	34.09674625915822	21.38473128900391
23	20.793606623023678	25.7211493354339	35.28797864189846	18.197265399643953
24	20.514692877478772	25.48695122043728	32.44933459548524	21.54902130659871
25	20.300536946888613	26.45085253144367	33.453000877943495	19.79560964372422
26	20.00233958566976	27.28092634816628	33.23876509801307	19.477968968150893
27	18.405552403724652	27.37914103669197	32.98045248225648	21.2348540773269
28	19.307730174706364	26.71243897015112	32.94515907385944	21.034671781283077
29	19.61710644356229	26.236776450193773	31.91087056940964	22.235246536834303
30	19.921452203981527	26.72034405483733	34.21771800965938	19.140485731521764
31	21.333388163213648	26.28245027282523	33.69282837142879	18.69133319253233
32	20.511898150569504	26.522836711692378	32.248273956698505	20.716991181039614
33	19.885879322893565	26.509781344559087	33.091403140554405	20.51293619199295
34	18.719679708326332	27.370557232613507	34.02799597719024	19.881767081869928
35	18.29927293183222	29.93867171420963	32.190742507037726	19.57131284692043
36	21.28036820127783	27.026406576072265	31.74350635221464	19.949718870435262
37	20.228552766639904	28.22490524877661	31.591153810989425	19.955388173594063
38	20.02841039526621	26.143352722083975	33.08824909161394	20.739987791035873
39	19.520049570470434	25.742988129996323	33.88802008370606	20.848942215827176
40	17.837983293122537	26.502075883223537	33.33574212176465	22.324198701889276
41	20.013438643966563	25.532425419718074	33.31581971136859	21.13831622494677
42	18.463642798767285	28.19472219815652	32.39036585769969	20.951269145376504
43	18.857819066984412	28.536996395201538	31.056562477916668	21.548622059897387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	314.0
2	424.0
3	1117.0
4	1810.0
5	1810.0
6	3266.5
7	4723.0
8	5493.0
9	6263.0
10	9665.0
11	13067.0
12	13067.0
13	24548.5
14	36030.0
15	60340.5
16	84651.0
17	80824.0
18	76997.0
19	76997.0
20	81584.0
21	86171.0
22	69444.5
23	52718.0
24	56309.5
25	59901.0
26	59901.0
27	63428.5
28	66956.0
29	68944.5
30	70933.0
31	76154.5
32	81376.0
33	81376.0
34	88376.0
35	95376.0
36	103263.0
37	111150.0
38	116293.5
39	121437.0
40	121437.0
41	125520.0
42	129603.0
43	133588.5
44	137574.0
45	152223.5
46	166873.0
47	166873.0
48	236882.5
49	306892.0
50	276467.0
51	246042.0
52	213060.0
53	180078.0
54	180078.0
55	150813.5
56	121549.0
57	97254.0
58	72959.0
59	62561.5
60	52164.0
61	52164.0
62	45316.5
63	38469.0
64	33209.5
65	27950.0
66	23428.5
67	18907.0
68	18907.0
69	16032.5
70	13158.0
71	10931.5
72	8705.0
73	7365.0
74	6025.0
75	6025.0
76	4730.5
77	3436.0
78	2706.5
79	1977.0
80	1513.0
81	1049.0
82	1049.0
83	776.0
84	503.0
85	431.0
86	359.0
87	245.0
88	131.0
89	131.0
90	112.5
91	94.0
92	58.5
93	23.0
94	16.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2504717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.39472403634004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50595586930234	28.957468464985553
2	13.487070899655881	10.626388723009917
3	5.165800453498453	6.105158498771153
4	2.4839897629675134	3.9142436488479566
5	1.3721217737203992	2.702717930998427
6	0.8327029391059129	1.9682461494196022
7	0.5402778184896646	1.4898866893649307
8	0.3763388354180307	1.186061165236073
9	0.2707793039756927	0.9600548359387182
>10	1.661217871477138	12.42617820054624
>50	0.1784905845132331	4.8628073542210615
>100	0.10868016081288796	7.9597108867596145
>500	0.007263793959617834	1.9606867817623028
>1k	0.007877635702684127	6.544046046455421
>5k	6.138417430662956E-4	1.6456339826125754
>10k+	8.184556574217275E-4	6.6907106410704715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39419	1.573790571948847	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38754	1.5472406663108047	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32425	1.2945574290428818	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13364	0.5335532916493161	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11046	0.4410079062824263	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10572	0.4220836126396715	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10409	0.41557589140809126	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10019	0.4000052700564575	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9784	0.39062297257534484	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8708	0.34766402751288866	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6472	0.25839246509685526	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5515	0.22018455578015403	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5335	0.21299811515632308	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5017	0.2003020700542217	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4723	0.1885642170352978	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4573	0.18257551651543868	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4249	0.16963992339254294	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3825	0.1527118632564078	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3789	0.1512745751316416	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3771	0.15055593106925852	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3647	0.14560527197284165	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3641	0.14536572395204728	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3277	0.1308331440238558	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3264	0.13031412331213468	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3202	0.12783879376392623	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3197	0.12763917041326425	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3090	0.12336723070909808	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3046	0.12161054522327272	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.11194877505123332	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	2794	0.1115495283499094	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2778	0.1109107336277911	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2771	0.11063126093686432	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2747	0.10967306885368686	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2575	0.10280602559091506	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2565	0.10240677888959113	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2506	0.10005122335177986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.9924670132394196E-5	0.0	0.0	0.0	0.0
6	7.984934026478839E-5	0.0	0.0	0.0	0.0
7	7.984934026478839E-5	0.0	0.0	0.0	0.0
8	1.197740103971826E-4	0.0	0.0	0.0	0.0
9	1.197740103971826E-4	0.0	0.0	7.984934026478839E-5	0.0
10	1.5969868052957678E-4	0.0	0.0	1.5969868052957678E-4	0.0
11	1.5969868052957678E-4	0.0	0.0	3.99246701323942E-4	0.0
12	1.5969868052957678E-4	0.0	0.0	7.98493402647884E-4	0.0
13	1.5969868052957678E-4	0.0	0.0	9.182674130450665E-4	0.0
14	1.99623350661971E-4	0.0	0.0	0.0012775894442366143	0.0
15	1.99623350661971E-4	0.0	0.0	0.0017167608156929506	0.0
16	1.99623350661971E-4	0.0	0.0	0.002235781527414075	0.0
17	1.99623350661971E-4	0.0	0.0	0.003193973610591536	0.0
18	1.99623350661971E-4	0.0	0.0	0.003832768332709843	0.0
19	1.99623350661971E-4	0.0	0.0	0.004950659096416881	0.0
20	1.99623350661971E-4	0.0	0.0	0.008384180727802781	0.0
21	1.99623350661971E-4	0.0	0.0	0.014093408556735153	0.0
22	1.99623350661971E-4	0.0	0.0	0.02327608268718582	0.0
23	1.99623350661971E-4	0.0	0.0	0.02930470787717734	0.0
24	2.395480207943652E-4	0.0	0.0	0.03697024454259703	0.0
25	2.395480207943652E-4	0.0	0.0	0.04283917105205898	0.0
26	2.794726909267594E-4	0.0	0.0	0.05441732539045329	0.0
27	2.794726909267594E-4	0.0	0.0	0.13434651499550648	0.0
28	2.794726909267594E-4	0.0	0.0	0.23771148596827507	0.0
29	2.794726909267594E-4	0.0	0.0	0.35556911219910275	0.0
30	3.99246701323942E-4	0.0	0.0	0.5683676040047638	0.0
31	4.391713714563362E-4	0.0	0.0	0.8432888825364303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9615	0.0	29.861675	1
TCACGTT	125	0.0	25.16	24
GTATTGG	850	0.0	22.417645	1
ATTGGAC	865	0.0	21.815031	3
CGCGATA	85	5.1868483E-8	21.764706	14
TTGGACC	1480	0.0	19.5	4
TGGACCC	1500	0.0	19.363335	5
GGACCCT	1535	0.0	18.68078	6
GACCCTC	1525	0.0	18.439344	7
ACGTGCG	105	4.8028414E-7	17.619047	9
TATTGGA	1090	0.0	17.311926	2
GTCCCGA	110	7.816652E-7	16.818182	11
GTATCAA	17185	0.0	16.73989	2
GTGCGCG	115	1.2435503E-6	16.086956	11
TGCGCGA	105	9.351823E-6	15.857142	12
GCGATAT	105	9.351823E-6	15.857142	15
TACTACG	105	9.351823E-6	15.857142	5
TTTTTAC	1955	0.0	15.6138115	1
GCGGACT	120	1.937622E-6	15.416666	37
ACGTTTA	205	5.456968E-12	15.341463	26
>>END_MODULE