FastQCFastQC Report
Wed 25 May 2016
SRR2088302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217318
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT442931.3767056908891195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT436251.3559430556755658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT372471.157703403891067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150390.4674390284081337No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG124670.3874966664781038No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA122710.3814046357867018No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA117990.3667340312645502No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG117580.36545967790563444No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG113090.3515039545360452No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA108980.33872933915764625No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA76780.23864597779889957No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66390.20635199877662075No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62420.1940125284476076No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA61080.18984756868920014No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56600.17592292710885277No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA56290.17495939164235552No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53890.16749976222431232No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG46440.14434382923913644No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43670.1357341736191449No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43520.13526794678051718No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42510.13212868606709066No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.12898942535366414No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39560.12295955824074586No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39220.12190277740652307No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG38630.12006895184125412No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36430.11323095820804782No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT35470.11024710644083054No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT33440.10393750322473565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105150.030.842131
GTATTGG10850.022.677421
ATTGGAC12350.020.0728743
GGACCCT18850.018.745366
TTGGACC20350.017.6363644
GTATCAA186150.017.481332
GACCCTC19950.017.1553887
CGTGTCT2850.016.87719335
TATTGGA14900.016.5134222
TGGACCC21550.016.48265
CTAGTAC4000.016.18753
TATACCG2350.015.7446815
CGCCGGA2300.015.28260914
CTAACGG1101.4534044E-515.13636427
TGCGATT1353.9783117E-715.07407529
CGACCGC2600.014.94230710
TTACGGG1001.0943353E-414.814
ACGTTTA1752.242814E-914.79999926
CCGACCG2700.014.3888899
ACCCTCG24050.014.3846158
Produced by FastQC (version 0.11.5)