##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3217318
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93166419980866	31.0	31.0	33.0	28.0	34.0
2	31.242330102277737	31.0	31.0	34.0	28.0	34.0
3	31.296261358062832	31.0	31.0	34.0	28.0	34.0
4	35.18728984825249	35.0	35.0	37.0	33.0	37.0
5	34.95470637344521	35.0	35.0	37.0	32.0	37.0
6	35.00880329516697	36.0	35.0	37.0	32.0	37.0
7	34.82041066503218	35.0	35.0	37.0	32.0	37.0
8	34.88671122966396	36.0	35.0	37.0	32.0	37.0
9	36.35115210868183	38.0	35.0	39.0	32.0	39.0
10	36.16328289587787	38.0	35.0	39.0	31.0	39.0
11	36.259297961842755	38.0	35.0	39.0	32.0	39.0
12	36.008754185939964	38.0	35.0	39.0	30.0	39.0
13	36.10211579955727	38.0	35.0	39.0	31.0	39.0
14	36.90291292312417	39.0	36.0	40.0	31.0	41.0
15	37.03405787056175	39.0	36.0	40.0	31.0	41.0
16	37.00452022460944	38.0	36.0	40.0	31.0	41.0
17	37.09478049729619	39.0	36.0	40.0	31.0	41.0
18	37.10994561308519	39.0	36.0	40.0	31.0	41.0
19	37.200410714763045	39.0	36.0	40.0	31.0	41.0
20	37.18182971033637	39.0	36.0	40.0	31.0	41.0
21	37.11931801581317	39.0	36.0	40.0	31.0	41.0
22	37.046054197937536	39.0	36.0	40.0	31.0	41.0
23	36.927303113960136	39.0	35.0	40.0	31.0	41.0
24	36.84223847316305	39.0	35.0	40.0	31.0	41.0
25	36.712597573506876	38.0	35.0	40.0	30.0	41.0
26	36.46560924347547	38.0	35.0	40.0	30.0	41.0
27	36.33093495886947	38.0	35.0	40.0	30.0	41.0
28	36.15787528618557	38.0	35.0	40.0	30.0	41.0
29	35.94562769362556	38.0	34.0	40.0	29.0	41.0
30	35.713807587562066	38.0	34.0	40.0	28.0	41.0
31	35.45380624482877	38.0	34.0	40.0	27.0	41.0
32	35.33991262287408	38.0	34.0	40.0	27.0	41.0
33	35.14516501011091	37.0	33.0	40.0	27.0	41.0
34	35.288957137591	38.0	34.0	40.0	27.0	41.0
35	35.20113523127027	38.0	34.0	40.0	26.0	41.0
36	35.11971088962919	38.0	34.0	40.0	26.0	41.0
37	34.99139252010526	38.0	33.0	40.0	25.0	41.0
38	34.934770203007595	38.0	33.0	40.0	25.0	41.0
39	34.80998707619203	38.0	33.0	40.0	24.0	41.0
40	34.59820011574858	38.0	33.0	40.0	23.0	41.0
41	34.4593108297035	38.0	33.0	40.0	23.0	41.0
42	34.28082862806847	38.0	33.0	40.0	22.0	41.0
43	33.8972460913096	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	9.0
16	38.0
17	112.0
18	268.0
19	648.0
20	1455.0
21	2793.0
22	5078.0
23	8727.0
24	14214.0
25	22350.0
26	33426.0
27	48049.0
28	67263.0
29	88992.0
30	112867.0
31	138404.0
32	166521.0
33	200035.0
34	238414.0
35	282791.0
36	346572.0
37	438447.0
38	517784.0
39	482056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22455971091449	22.571253447747473	14.761083610634696	22.443103230703336
2	18.43535516228113	23.98040852660508	35.77712865187712	21.807107659236667
3	19.580408277950763	25.127792776467857	33.67133743074201	21.620461514839377
4	13.322867058835964	16.514500587134997	36.48784484468119	33.67478750934785
5	11.850429457081955	38.93991827975973	35.49288568926043	13.716766573897887
6	31.413804914528185	37.43565292582207	13.526173042266882	17.624369117382862
7	24.311833645290893	34.6020816095891	21.882791816040566	19.20329292907944
8	26.341474482783482	34.623683453112186	19.513924330762457	19.520917733341868
9	23.724356746830747	14.584197148059348	23.45988180217187	38.23156430293804
10	15.975977506730763	27.721878906592384	34.30465996833387	21.99748361834298
11	31.81047070883264	22.54800426939457	24.900615978899197	20.740909042873596
12	20.896069334768896	27.406864972626266	31.696276215158093	20.00078947744674
13	31.45287472360519	21.40963373841193	27.82143387753402	19.316057660448855
14	19.813148715793712	23.62595180209106	29.18427709042128	27.37662239169395
15	21.32574398924819	29.917527580425684	27.299508472584925	21.4572199577412
16	20.78669251842684	28.93425517775986	28.0874007480765	22.191651555736797
17	20.654346259835055	26.96304810404194	27.936716233832033	24.445889402290977
18	22.62054916548504	25.263526950086995	31.507547590881597	20.608376293546364
19	21.846923431255476	24.560798777118084	32.88614927091447	20.70612852071197
20	22.998969949504524	23.86904247575154	33.05818697436809	20.07380060037584
21	21.77773536840312	24.84936210843939	33.62036329638538	19.75253922677211
22	19.752072999933485	25.308782035223125	33.045381277200455	21.893763687642938
23	21.345232271102827	25.572044790101568	34.24078689144188	18.841936047353727
24	21.163310558670297	25.413558746757392	31.482775404855843	21.940355289716464
25	20.879347332156783	26.132698104446	32.627393375476096	20.36056118792112
26	20.66149507136068	27.029998278068877	32.27958815385983	20.028918496710617
27	19.092206614329076	27.10959874031725	31.947572481178423	21.850622164175252
28	19.979249797502145	26.305077707581283	32.12933878466474	21.586333710251832
29	20.26899423681464	26.083992940704025	31.08853399011226	22.558478832369072
30	20.57229033623658	26.41290043446125	33.234980191575715	19.779829037726454
31	21.819011984516294	25.935732806020418	32.847172707205196	19.39808250225809
32	20.877699997326964	26.19737930785828	31.445011030927	21.479909663887746
33	20.522714882395835	26.014991368587125	32.30687796481418	21.155415784202866
34	19.38863363832857	26.94331116787336	33.14453218488194	20.523523008916122
35	18.971951171752373	29.341302289671084	31.50571376531633	20.181032773260213
36	21.86488870543726	26.555907746762987	31.08194465079299	20.497258897006763
37	20.838816678985417	27.583564944466165	30.94664562222323	20.630972754325185
38	20.564955033975505	25.74283922198552	32.36235895861086	21.32984678542811
39	20.03827411527241	25.412284393398476	33.231809849073045	21.317631642256067
40	18.472093837164994	26.17639910012004	32.76685736380426	22.58464969891071
41	20.56806321289969	25.13854707554553	32.68850017312557	21.604889538429216
42	19.044371740685875	27.63612425007413	31.820416881390027	21.49908712784997
43	19.37181839034873	27.874055346720468	30.588800982681846	22.16532528024895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	347.5
2	482.0
3	1393.5
4	2305.0
5	2305.0
6	3896.5
7	5488.0
8	6279.5
9	7071.0
10	10858.0
11	14645.0
12	14645.0
13	27581.5
14	40518.0
15	67230.5
16	93943.0
17	90899.5
18	87856.0
19	87856.0
20	93548.0
21	99240.0
22	80589.0
23	61938.0
24	66834.0
25	71730.0
26	71730.0
27	76606.5
28	81483.0
29	84861.0
30	88239.0
31	94896.0
32	101553.0
33	101553.0
34	111333.0
35	121113.0
36	132300.0
37	143487.0
38	149774.0
39	156061.0
40	156061.0
41	162335.0
42	168609.0
43	174680.0
44	180751.0
45	198141.0
46	215531.0
47	215531.0
48	297303.5
49	379076.0
50	343958.0
51	308840.0
52	272327.0
53	235814.0
54	235814.0
55	201618.5
56	167423.0
57	137608.5
58	107794.0
59	94776.0
60	81758.0
61	81758.0
62	71700.5
63	61643.0
64	53037.0
65	44431.0
66	37764.0
67	31097.0
68	31097.0
69	26504.5
70	21912.0
71	18122.5
72	14333.0
73	11972.0
74	9611.0
75	9611.0
76	7407.0
77	5203.0
78	4021.0
79	2839.0
80	2230.5
81	1622.0
82	1622.0
83	1215.5
84	809.0
85	627.0
86	445.0
87	346.5
88	248.0
89	248.0
90	181.5
91	115.0
92	74.0
93	33.0
94	21.5
95	10.0
96	10.0
97	6.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3217318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.78795251667981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42939808332095	28.093880536675524
2	13.90978869171786	10.790644465828043
3	5.397554940244765	6.280803141851534
4	2.5690986608685416	3.9860030747373867
5	1.4653496942140527	2.8418957179752975
6	0.9024665650205892	2.100289816314585
7	0.6190811748177416	1.6809023848960638
8	0.4091705870132978	1.2696711440235038
9	0.3112484741884863	1.086542193394084
>10	1.675436868450477	12.279468844639108
>50	0.1738242358755395	4.705700884521112
>100	0.12125792776355299	8.967725797768527
>500	0.008242860611047393	2.180202083807797
>1k	0.006707425791322361	5.772924604998339
>5k	5.65686512521163E-4	1.3586689381262684
>10k+	8.081235893159472E-4	6.6046763704425775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44293	1.3767056908891195	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43625	1.3559430556755658	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	37247	1.157703403891067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15039	0.4674390284081337	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12467	0.3874966664781038	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12271	0.3814046357867018	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11799	0.3667340312645502	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11758	0.36545967790563444	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11309	0.3515039545360452	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10898	0.33872933915764625	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7678	0.23864597779889957	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6639	0.20635199877662075	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6242	0.1940125284476076	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6108	0.18984756868920014	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5660	0.17592292710885277	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5629	0.17495939164235552	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5389	0.16749976222431232	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4644	0.14434382923913644	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4367	0.1357341736191449	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4352	0.13526794678051718	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4251	0.13212868606709066	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4150	0.12898942535366414	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3956	0.12295955824074586	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3922	0.12190277740652307	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3863	0.12006895184125412	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3643	0.11323095820804782	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3547	0.11024710644083054	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3344	0.10393750322473565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.108178924184678E-5	0.0
5	0.0	0.0	0.0	3.108178924184678E-5	0.0
6	0.0	0.0	0.0	3.108178924184678E-5	0.0
7	0.0	0.0	0.0	3.108178924184678E-5	0.0
8	0.0	0.0	0.0	3.108178924184678E-5	0.0
9	0.0	0.0	0.0	9.324536772554034E-5	0.0
10	0.0	0.0	0.0	2.7973610317662105E-4	0.0
11	0.0	0.0	0.0	5.283904171113952E-4	0.0
12	0.0	0.0	0.0	7.459629418043228E-4	0.0
13	0.0	0.0	0.0	9.013718880135567E-4	0.0
14	0.0	0.0	0.0	0.0011811079911901777	0.0
15	0.0	0.0	0.0	0.001554089462092339	0.0
16	0.0	0.0	0.0	0.0023932977716222023	0.0
17	0.0	0.0	0.0	0.003108178924184678	0.0
18	0.0	0.0	0.0	0.00357440576281238	0.0
19	0.0	0.0	0.0	0.0048487591217280974	0.0
20	0.0	0.0	0.0	0.00829883772757309	0.0
21	0.0	0.0	0.0	0.013800314423379971	0.0
22	0.0	0.0	0.0	0.021881579626260132	0.0
23	0.0	0.0	0.0	0.0270411566404067	0.0
24	0.0	0.0	0.0	0.03493593110783578	0.0
25	0.0	0.0	0.0	0.040188753489707886	0.0
26	0.0	0.0	0.0	0.05088088898890318	0.0
27	0.0	0.0	0.0	0.13989913337755236	0.0
28	0.0	0.0	0.0	0.2485610685670487	0.0
29	0.0	0.0	0.0	0.36645429516137357	0.0
30	0.0	0.0	0.0	0.5771577444318529	0.0
31	0.0	0.0	0.0	0.863358859770778	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10515	0.0	30.84213	1
GTATTGG	1085	0.0	22.67742	1
ATTGGAC	1235	0.0	20.072874	3
GGACCCT	1885	0.0	18.74536	6
TTGGACC	2035	0.0	17.636364	4
GTATCAA	18615	0.0	17.48133	2
GACCCTC	1995	0.0	17.155388	7
CGTGTCT	285	0.0	16.877193	35
TATTGGA	1490	0.0	16.513422	2
TGGACCC	2155	0.0	16.4826	5
CTAGTAC	400	0.0	16.1875	3
TATACCG	235	0.0	15.744681	5
CGCCGGA	230	0.0	15.282609	14
CTAACGG	110	1.4534044E-5	15.136364	27
TGCGATT	135	3.9783117E-7	15.074075	29
CGACCGC	260	0.0	14.942307	10
TTACGGG	100	1.0943353E-4	14.8	14
ACGTTTA	175	2.242814E-9	14.799999	26
CCGACCG	270	0.0	14.388889	9
ACCCTCG	2405	0.0	14.384615	8
>>END_MODULE