FastQCFastQC Report
Wed 25 May 2016
SRR2088301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871745
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106951.2268495947782894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105941.2152636378757549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83650.9595695989079376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.4112441138176875No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27570.31626220970582No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA27380.3140826732588085No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25670.2944668452357054No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG25550.2930902959007508No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24090.2763422789921365No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23180.2659034465353974No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13750.15772961129688154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.15222341395706313No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12860.14752020372930158No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12090.1386873454966762No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11770.1350165472701306No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11410.13088689926526678No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.12446300236881198No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10450.11987450458562997No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10040.11517129435786842No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9000.1032412001215952No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8800.10094695123000419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.031.2910181
CGAGACG250.005495625529.59999831
GCCGAGT753.7386235E-722.212
TGCGGTC450.003825077320.55555712
TTGGACC4000.019.8874994
GGACCCT4100.019.4024416
TAAACGT701.21876816E-418.54
CGCTGAA801.61594E-518.526
CGGGCGA701.21876816E-418.528
AACGAGA701.21876816E-418.536
ATAAGAC609.2343724E-418.53
GCGATCT801.61594E-518.528
CGCAAAT701.21876816E-418.517
TACACTC902.1510023E-618.55
GTCAATC500.00703355418.49999816
GTAACAC500.00703355418.4999983
TGGACCC4650.018.3010755
GTATTGG2650.018.1509441
GACCCTC4600.018.0978247
GTATCAA44950.017.862072
Produced by FastQC (version 0.11.5)