FastQCFastQC Report
Wed 25 May 2016
SRR2088300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623973
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124882.0013686489639775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116601.8686705995291462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77091.2354701245085924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43910.703716346700899No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA29540.4734179203266808No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27660.44328841151780607No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26120.41860785642968523No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA24070.38575387076043355No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21270.34088013423657754No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18620.2984103478836424No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.2633126753881979No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12890.20657945135446565No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12840.20577813463082537No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12770.204656291217729No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.20080997094425562No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12290.19696365067078223No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10870.17420625571939813No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9510.15241044083638236No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9440.15128859742328593No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8870.14215358677378667No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.14071121667123418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8710.13958937325813778No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8460.13558278963993634No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG8100.12981330922972628No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8090.12965304588499824No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT7940.1272490957140774No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7940.1272490957140774No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT7870.12612725230098096No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA7110.11394723810164863No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT7110.11394723810164863No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7090.11362671141219251No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.11026118117290333No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA6600.10577380752051771No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC6550.10497249079687743No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT6440.1032095940048688No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA6440.1032095940048688No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6300.10096590717867598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.030.7299821
TAGGTCC451.3222653E-424.6666664
CGTTTAT450.003823877820.5555551
CGGTTGA555.1394734E-420.1818181
TATAGAG555.1394734E-420.1818185
TATCCTG555.1394734E-420.1818185
GTATCAA41450.019.9505422
GTATTGG1900.018.51
TATGCTA701.2180638E-418.55
GTAGAAC500.007031365318.53
CAATAGT500.007031365318.54
ATACCCT2750.018.16363737
GATACCC2650.017.45283136
TGGACCC3400.017.4117645
GGCTAGA650.001578839817.0769231
ATTGGAC1851.8189894E-1217.03
TTGGACC3300.016.8181824
AACCATG1107.793533E-716.8181827
TTAATAC1452.9704097E-916.5862063
TAATACC904.4407185E-516.4444454
Produced by FastQC (version 0.11.5)