FastQCFastQC Report
Wed 25 May 2016
SRR2088299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162583
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415671.314337046648262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT412521.3043768337463397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT365601.156017091092945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142950.4520039474062815No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG124100.3924007686122388No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA123640.39094626133132315No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG123360.39006090907337454No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG118210.37377675147181905No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA116930.36972942686405386No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA114930.36340548216442065No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA77080.24372482872386272No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66850.211377851585239No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA64600.20426341379815172No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC64520.20401045601016637No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA57590.1820979876259374No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA54480.17226425361800782No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.1674580556462866No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG46840.14810678486540907No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43930.1389054453274428No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42730.13511107850766288No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42590.13466840237868855No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40410.12777530265608839No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38610.12208375242641853No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG38200.12078734376299372No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.11550684993880003No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35750.11304051150594309No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT33550.10608417233634658No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT32700.1033964958390025No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA31680.10017128404218956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106050.030.562941
GTATTGG8500.021.9823531
ACGTTTA2750.019.50909226
TTGGACC18050.018.1412734
ATTGGAC10250.017.6878053
GTATCAA185400.017.5121362
CGGCGTA752.0687093E-417.26666612
GGACCCT18950.017.0844336
TCACGTT3250.016.50769224
TGGACCC20550.015.8442835
CGTTTAT3650.015.7123327
TAGGTCG1659.767973E-1015.6969721
GACCCTC20350.015.5454557
TATTGGA13150.015.0532322
CTCGACG1406.0039383E-714.53571418
TACGTTA1406.0039383E-714.53571419
CTGTGCG3200.014.4531259
TCGACGG1458.9234163E-714.03448319
ATTAGAC2650.013.9622643
GATACTG11600.013.87536
Produced by FastQC (version 0.11.5)