FastQCFastQC Report
Wed 25 May 2016
SRR2088298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886348
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT450531.5608997944807765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT438991.5209184755268594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360341.2484288103859964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157050.5441131838572479No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG142270.4929066072420928No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA137900.4777663677422127No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA120370.41703218045779655No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA118960.4121471146237391No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG118690.41121167648530255No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG104790.36305393528431085No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69280.24002649715141763No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA62630.2169870022602957No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62510.21657125197654614No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA56330.19516011236344336No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56070.19425932008198596No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.19297742337375812No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53530.18545927240928675No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG51020.17676316230752492No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG46290.16037567195639613No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45050.1560795856909839No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC44200.15313468784775777No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43160.14953151872192819No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC42380.146829141877556No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39770.13778657320600288No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.1375786980641281No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG34810.12060222814435405No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33410.11575180816727575No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA33080.11460849488696442No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC32420.11232186832634179No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG30950.10722892735040958No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT30750.10653601021082698No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA30620.10608561407009828No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT29780.10317536208385128No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA29270.10140842337791561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100600.031.1153071
GTATTGG8950.022.737431
ATTGGAC9900.020.9292933
TTGGACC18000.018.3972234
GTATCAA176050.017.8011932
GGACCCT18000.017.7805566
TGGACCC18200.017.6868135
CGTGTCT3050.016.98360635
TATTGGA12500.016.5762
GACCCTC18850.016.4880627
TTTTTAC21600.016.0162051
TATACCG1557.221388E-915.5161295
GATACCT12150.015.378636
CGAATTA1455.3541953E-815.31034515
TTGATAC32350.015.26893334
GATACTG10100.015.01980236
AAACGCG750.00410640514.85
ATACCTG13650.014.36630237
ACGTTTA2450.014.3469426
TGATACC23900.014.24267735
Produced by FastQC (version 0.11.5)