##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2886348
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.70744969075108	31.0	30.0	31.0	28.0	34.0
2	30.998343928036398	31.0	31.0	34.0	28.0	34.0
3	31.024435722927382	31.0	31.0	34.0	27.0	34.0
4	34.92192278962897	35.0	35.0	37.0	32.0	37.0
5	34.680869735735264	35.0	35.0	37.0	32.0	37.0
6	34.770182943983194	35.0	35.0	37.0	32.0	37.0
7	34.56859533223298	35.0	35.0	37.0	30.0	37.0
8	34.657817075418485	35.0	35.0	37.0	30.0	37.0
9	36.073520933719706	38.0	35.0	39.0	31.0	39.0
10	35.84912872598869	37.0	35.0	39.0	30.0	39.0
11	35.97338262745864	37.0	35.0	39.0	30.0	39.0
12	35.720194515699426	37.0	35.0	39.0	30.0	39.0
13	35.81054467444674	37.0	35.0	39.0	30.0	39.0
14	36.556917946138164	38.0	35.0	40.0	30.0	41.0
15	36.67343230961755	38.0	35.0	40.0	31.0	41.0
16	36.623158745930844	38.0	35.0	40.0	30.0	41.0
17	36.73739549077242	38.0	36.0	40.0	31.0	41.0
18	36.74782042913744	38.0	36.0	40.0	31.0	41.0
19	36.85347747395671	39.0	36.0	40.0	31.0	41.0
20	36.83467551383271	39.0	36.0	40.0	31.0	41.0
21	36.758593211906536	39.0	35.0	40.0	30.0	41.0
22	36.67455414246653	38.0	35.0	40.0	30.0	41.0
23	36.53734269048638	38.0	35.0	40.0	30.0	41.0
24	36.45602401373639	38.0	35.0	40.0	30.0	41.0
25	36.319697763402054	38.0	35.0	40.0	30.0	41.0
26	36.03729938316516	38.0	34.0	40.0	29.0	41.0
27	35.89534872440884	38.0	34.0	40.0	29.0	41.0
28	35.70720474454224	38.0	34.0	40.0	28.0	41.0
29	35.49474422349627	38.0	34.0	40.0	27.0	41.0
30	35.25825749355241	38.0	34.0	40.0	27.0	41.0
31	34.959753640240194	37.0	33.0	40.0	26.0	41.0
32	34.85220908913271	37.0	33.0	40.0	26.0	41.0
33	34.646991284488216	37.0	33.0	40.0	25.0	41.0
34	34.78180940066825	37.0	33.0	40.0	25.0	41.0
35	34.67738124439603	37.0	33.0	40.0	25.0	41.0
36	34.593429482515624	37.0	33.0	40.0	25.0	41.0
37	34.469682796391844	37.0	33.0	40.0	24.0	41.0
38	34.38778865195742	37.0	33.0	40.0	24.0	41.0
39	34.25341712087385	37.0	33.0	40.0	23.0	41.0
40	34.0277121816219	37.0	33.0	40.0	22.0	41.0
41	33.86665883670299	37.0	33.0	40.0	21.0	41.0
42	33.661089030151594	37.0	32.0	40.0	20.0	41.0
43	33.271510919681205	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	20.0
16	49.0
17	136.0
18	348.0
19	855.0
20	1689.0
21	3475.0
22	6207.0
23	10619.0
24	16900.0
25	25232.0
26	37256.0
27	52180.0
28	70583.0
29	91411.0
30	113905.0
31	136539.0
32	161321.0
33	190818.0
34	224818.0
35	263060.0
36	319345.0
37	396753.0
38	453227.0
39	309595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.96811888240781	23.5682946061944	14.894635019755068	21.568951491642725
2	19.188988992318322	24.91667671396519	34.83633990080198	21.057994392914505
3	20.659116641513776	25.7037266469601	33.65176340482852	19.9853933066976
4	13.844934845001363	17.093954020790285	36.40264444897151	32.658466685236846
5	11.446887208333854	39.762149262666874	35.4701512083782	13.320812320621075
6	31.15497507577049	37.64158029454522	13.21188574627869	17.991558883405602
7	23.971191277004714	34.93650107332865	21.86770271637377	19.224604933292866
8	26.600014967010218	34.62236708809887	19.48760163362145	19.290016311269465
9	23.576609611869394	14.33260992783961	23.592061664082088	38.49871879620891
10	15.882630923228938	27.939146630967578	34.45312207675582	21.725100369047667
11	31.537742503675926	22.41455292293237	25.22672248807143	20.82098208532027
12	20.72269178907048	27.563550895456817	32.07277847300464	19.64097884246806
13	31.992711897525872	21.02431861993079	28.293608393721065	18.689361088822277
14	19.736843928729314	23.51923607271195	29.4255924788002	27.318327519758533
15	21.19023762900385	29.895598174579085	27.805552206456046	21.108611989961016
16	20.369650506453137	29.002843731940846	28.78277324840941	21.844732513196607
17	20.514296959341006	26.789458512972104	28.377589951038473	24.318654576648417
18	22.44032944052484	24.856011818394734	32.11670941965418	20.586949321426246
19	21.51937327030559	23.97229994442805	33.793846064299935	20.714480720966424
20	22.8987634200727	23.399499990992076	33.959730427516014	19.742006161419205
21	21.802499213539047	24.303964733289263	34.67003978730215	19.223496265869535
22	19.41806739866433	24.80910132804499	34.00982833670784	21.763002936582836
23	21.24511666645879	25.15379295913036	35.31441808125701	18.286672293153842
24	20.95658596953659	24.787482313290013	32.328915293651356	21.92701642352204
25	20.707828716426434	25.82159185240311	33.35037909496707	20.120200336203396
26	20.466208509853974	26.605003970415208	33.17382380780142	19.7549637119294
27	18.83379966656827	26.932788423294767	32.89495930497639	21.338452605160573
28	19.954523848129195	25.99312349030678	32.90566487478294	21.146687786781083
29	20.01432259727517	25.73944652550559	31.934749378799783	22.311481498419454
30	20.35835595707794	26.09189882855428	34.136528235680515	19.413216978687252
31	21.711172734542057	25.690526575451056	33.60350172605659	18.994798963950295
32	21.054945557500343	25.85693062652182	31.980759076868072	21.10736473910977
33	20.53487659838661	25.99433609530105	32.7616420473207	20.70914525899164
34	19.32057395712506	27.129611536793206	33.61365296215148	19.936161543930254
35	18.707134413452568	29.93041726084311	31.927161936121358	19.435286389582963
36	21.894969005816346	26.686872130456894	31.51809830276876	19.900060560958
37	20.824065566591415	28.16614628589484	31.161245975883713	19.848542171630033
38	20.779545640373236	25.89344735977782	32.68892039352151	20.63808660632744
39	20.200751953679873	25.762312791111814	33.437721300411454	20.59921395479686
40	18.43706995830025	26.78834984554877	32.45083406436092	22.323746131790067
41	20.9935877447903	25.576437768418774	32.419029167654074	21.010945319136848
42	19.158569929890643	28.405479865906674	31.55582764101903	20.88012256318365
43	19.70919653485997	28.649767803466524	30.03577531191665	21.605260349756854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	331.0
2	395.0
3	1060.5
4	1726.0
5	1726.0
6	3384.5
7	5043.0
8	5561.5
9	6080.0
10	9591.0
11	13102.0
12	13102.0
13	25910.0
14	38718.0
15	64883.5
16	91049.0
17	87215.0
18	83381.0
19	83381.0
20	89471.5
21	95562.0
22	76640.0
23	57718.0
24	61802.5
25	65887.0
26	65887.0
27	70848.5
28	75810.0
29	79061.0
30	82312.0
31	88467.5
32	94623.0
33	94623.0
34	102867.5
35	111112.0
36	119780.5
37	128449.0
38	133482.0
39	138515.0
40	138515.0
41	142840.0
42	147165.0
43	152204.5
44	157244.0
45	171766.0
46	186288.0
47	186288.0
48	264672.0
49	343056.0
50	313012.0
51	282968.0
52	249503.5
53	216039.0
54	216039.0
55	182288.0
56	148537.0
57	119753.5
58	90970.0
59	78896.5
60	66823.0
61	66823.0
62	58035.0
63	49247.0
64	42700.0
65	36153.0
66	30592.0
67	25031.0
68	25031.0
69	21498.0
70	17965.0
71	15150.5
72	12336.0
73	9902.0
74	7468.0
75	7468.0
76	6004.5
77	4541.0
78	3428.5
79	2316.0
80	1769.5
81	1223.0
82	1223.0
83	933.0
84	643.0
85	491.0
86	339.0
87	239.5
88	140.0
89	140.0
90	107.5
91	75.0
92	47.5
93	20.0
94	14.0
95	8.0
96	8.0
97	5.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2886348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.37426157623207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9317685542389	29.8494056240229
2	13.556000320526964	10.94627005736883
3	5.148172682505047	6.235610115692134
4	2.3389281799398725	3.7772999257965125
5	1.2523122147652999	2.528059046702237
6	0.7848699629482554	1.901312711244026
7	0.49520600359898426	1.399550370639814
8	0.3506576199108845	1.1326033975984813
9	0.2702602159322008	0.9820400986526074
>10	1.5971179528895454	12.299796233752522
>50	0.15893292078976962	4.4615724808581145
>100	0.10042940863573008	7.541869504612429
>500	0.007413375535939586	2.0593194006650335
>1k	0.006378951042552121	5.7773706356324706
>5k	6.896163289245536E-4	1.6255641583041314
>10k+	8.62020411155692E-4	7.4823562384577675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45053	1.5608997944807765	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43899	1.5209184755268594	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36034	1.2484288103859964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15705	0.5441131838572479	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14227	0.4929066072420928	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13790	0.4777663677422127	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12037	0.41703218045779655	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11896	0.4121471146237391	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11869	0.41121167648530255	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10479	0.36305393528431085	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6928	0.24002649715141763	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6263	0.2169870022602957	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6251	0.21657125197654614	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5633	0.19516011236344336	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5607	0.19425932008198596	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5570	0.19297742337375812	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5353	0.18545927240928675	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5102	0.17676316230752492	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4629	0.16037567195639613	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4505	0.1560795856909839	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4420	0.15313468784775777	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4316	0.14953151872192819	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4238	0.146829141877556	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3977	0.13778657320600288	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3971	0.1375786980641281	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3481	0.12060222814435405	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3341	0.11575180816727575	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3308	0.11460849488696442	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3242	0.11232186832634179	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3095	0.10722892735040958	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3075	0.10653601021082698	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3062	0.10608561407009828	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2978	0.10317536208385128	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2927	0.10140842337791561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.464585697913072E-5	0.0	0.0	0.0	0.0
2	3.464585697913072E-5	0.0	0.0	0.0	0.0
3	6.929171395826145E-5	1.0393757093739216E-4	0.0	0.0	0.0
4	6.929171395826145E-5	1.0393757093739216E-4	0.0	3.464585697913072E-5	0.0
5	6.929171395826145E-5	1.0393757093739216E-4	0.0	3.464585697913072E-5	0.0
6	1.385834279165229E-4	1.0393757093739216E-4	0.0	3.464585697913072E-5	0.0
7	1.732292848956536E-4	1.0393757093739216E-4	0.0	3.464585697913072E-5	0.0
8	2.4252099885391505E-4	1.0393757093739216E-4	0.0	6.929171395826145E-5	0.0
9	2.4252099885391505E-4	1.0393757093739216E-4	0.0	6.929171395826145E-5	0.0
10	2.4252099885391505E-4	1.0393757093739216E-4	0.0	1.0393757093739216E-4	0.0
11	2.4252099885391505E-4	1.0393757093739216E-4	0.0	2.0787514187478433E-4	0.0
12	2.4252099885391505E-4	1.0393757093739216E-4	0.0	4.850419977078301E-4	0.0
13	3.118127128121765E-4	1.732292848956536E-4	0.0	5.196878546869609E-4	0.0
14	3.118127128121765E-4	1.732292848956536E-4	0.0	6.23625425624353E-4	0.0
15	3.118127128121765E-4	1.732292848956536E-4	0.0	0.0010393757093739217	0.0
16	3.118127128121765E-4	1.732292848956536E-4	0.0	0.0015244177070817518	0.0
17	3.464585697913072E-4	1.732292848956536E-4	0.0	0.0021826889896852356	0.0
18	3.464585697913072E-4	3.464585697913072E-4	0.0	0.0025291475594765426	0.0
19	4.503961407286994E-4	5.543337116660916E-4	0.0	0.0030834812711426343	0.0
20	4.503961407286994E-4	6.23625425624353E-4	0.0	0.004989003404994824	0.0
21	4.503961407286994E-4	6.582712826034837E-4	0.0	0.009770131668114863	0.0
22	4.503961407286994E-4	6.582712826034837E-4	0.0	0.016144969352274916	0.0
23	4.503961407286994E-4	6.582712826034837E-4	0.0	0.020094597047895818	0.0
24	4.503961407286994E-4	6.582712826034837E-4	0.0	0.02442532917028716	0.0
25	4.503961407286994E-4	6.582712826034837E-4	0.0	0.028063144153095885	0.0
26	4.850419977078301E-4	6.582712826034837E-4	0.0	0.034645856979130724	0.0
27	5.196878546869609E-4	6.582712826034837E-4	0.0	0.08460518274303722	0.0
28	5.196878546869609E-4	6.582712826034837E-4	0.0	0.14991262314869863	0.0
29	5.543337116660916E-4	6.582712826034837E-4	0.0	0.22405475708403838	0.0
30	5.543337116660916E-4	6.929171395826144E-4	0.0	0.3610791214365004	0.0
31	6.23625425624353E-4	6.929171395826144E-4	0.0	0.5145256219970703	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10060	0.0	31.115307	1
GTATTGG	895	0.0	22.73743	1
ATTGGAC	990	0.0	20.929293	3
TTGGACC	1800	0.0	18.397223	4
GTATCAA	17605	0.0	17.801193	2
GGACCCT	1800	0.0	17.780556	6
TGGACCC	1820	0.0	17.686813	5
CGTGTCT	305	0.0	16.983606	35
TATTGGA	1250	0.0	16.576	2
GACCCTC	1885	0.0	16.488062	7
TTTTTAC	2160	0.0	16.016205	1
TATACCG	155	7.221388E-9	15.516129	5
GATACCT	1215	0.0	15.3786	36
CGAATTA	145	5.3541953E-8	15.310345	15
TTGATAC	3235	0.0	15.268933	34
GATACTG	1010	0.0	15.019802	36
AAACGCG	75	0.004106405	14.8	5
ATACCTG	1365	0.0	14.366302	37
ACGTTTA	245	0.0	14.34694	26
TGATACC	2390	0.0	14.242677	35
>>END_MODULE