FastQCFastQC Report
Wed 25 May 2016
SRR2088297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510937
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT454961.2958364106220077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT447801.275442994277596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397891.1332872107930163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159900.45543397674182134No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA147240.4193752266132944No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG138810.39536454228600515No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG126440.36013178248427696No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA123020.35039079311306354No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG117280.3340418811274597No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA115710.32957014039272137No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA80410.22902717992376395No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA79020.2250681228401421No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA69160.19698445172898288No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC68390.19479130499920677No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60050.17103696249747574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57570.1639733210820929No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA57460.1636600144064106No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG50310.14329508048706086No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG49150.13999111917986565No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48960.13944995310368713No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45490.12956655160716357No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC43570.12409792599525427No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC42180.12013886891163242No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40950.11663553062900302No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.11182200079352035No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG38730.1103124322652329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111450.029.1816961
GTATTGG11850.023.1054841
ATTGGAC12250.021.7469393
TTGGACC19900.020.266334
GGACCCT21050.018.5439436
TGGACCC22400.018.417415
GTATCAA194750.016.8138642
GACCCTC23700.016.0801687
TATTGGA17450.016.0085952
TTTTTAC23150.015.3434131
CTAGTAC4600.014.8804353
TACGACT1001.0943808E-414.79999920
CGGTATA2006.184564E-1114.79999926
ATTCGCG908.279986E-414.38888817
ACGTTTA2252.7284841E-1113.977777526
ATTGCGG800.0063020213.8750018
ACCCTCG26800.013.8758
CCCTCGC27000.013.8407419
AACACCG2151.9826984E-1013.7674425
TACTAGG2750.013.4545452
Produced by FastQC (version 0.11.5)