FastQCFastQC Report
Wed 25 May 2016
SRR2088296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876668
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT378961.317357442708022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364301.2663957050309596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327421.1381918247083085No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA144920.5037772867776191No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128360.4462106854179905No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG126050.43818056167760755No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA108100.3757819810975754No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA105900.36813424420197255No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG105320.3661180226567682No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG100920.3508225488655625No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA80570.2800809825812363No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA62080.215805230217738No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60830.21145992516341822No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC56760.197311611906553No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52360.18201613811534734No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49440.1718655055084563No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG48110.16724210093066005No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.16334870760198952No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG46930.16314013295938218No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG46320.1610196240928741No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC40560.14099645840256853No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40540.1409269335216994No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36730.1276824437161327No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36490.12684814514570328No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG31190.1084240517153874No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG30740.10685974189583226No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.10571258136149184No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA29900.10393969689932936No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC29510.10258396172238159No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA29360.10206252511586321No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA29280.10178442559238676No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA28970.10070678993891545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93000.029.520431
GTATTGG11350.022.656391
ACGTTTA1700.020.67647226
ATTGGAC12600.020.2619043
TTGGACC20500.018.6804894
TCACGTT2500.017.7624
GGACCCT21050.017.4014246
TATTGGA15100.017.1523192
TGGACCC21950.017.1093395
GTATCAA162550.016.9806212
CCGACCG2300.016.8913049
GACCCTC22250.015.9640457
ATTAGAC2003.6379788E-1215.7249993
TATACTG4500.015.6222225
TTTTTAC21550.015.5382831
CGACGAG1101.4532976E-515.13636424
GACGTCG1101.4532976E-515.13636436
GATACTG10400.015.12019336
TATACCG2109.094947E-1214.976195
TCGAACC4700.014.95744631
Produced by FastQC (version 0.11.5)