##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2876668
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.944669318809122	31.0	31.0	33.0	28.0	34.0
2	31.267027338573655	31.0	31.0	34.0	28.0	34.0
3	31.3307468918902	31.0	31.0	34.0	28.0	34.0
4	35.2154583010622	35.0	35.0	37.0	33.0	37.0
5	34.982338942137226	35.0	35.0	37.0	32.0	37.0
6	35.0311575753615	36.0	35.0	37.0	32.0	37.0
7	34.83376983371039	35.0	35.0	37.0	32.0	37.0
8	34.89990468138833	36.0	35.0	37.0	32.0	37.0
9	36.37110295661508	38.0	35.0	39.0	32.0	39.0
10	36.17788636019172	38.0	35.0	39.0	32.0	39.0
11	36.28248098146884	38.0	35.0	39.0	32.0	39.0
12	36.036233586913745	38.0	35.0	39.0	30.0	39.0
13	36.114065648173515	38.0	35.0	39.0	31.0	39.0
14	36.9153416383121	39.0	36.0	40.0	31.0	41.0
15	37.04781365107131	39.0	36.0	40.0	31.0	41.0
16	37.015832901120326	39.0	36.0	40.0	31.0	41.0
17	37.107536914235496	39.0	36.0	40.0	31.0	41.0
18	37.11554757100924	39.0	36.0	40.0	31.0	41.0
19	37.20771044833815	39.0	36.0	40.0	31.0	41.0
20	37.192646840024636	39.0	36.0	40.0	31.0	41.0
21	37.117854754180875	39.0	36.0	40.0	31.0	41.0
22	37.036356645952885	39.0	36.0	40.0	31.0	41.0
23	36.910062961732116	39.0	35.0	40.0	31.0	41.0
24	36.82695882875605	39.0	35.0	40.0	30.0	41.0
25	36.71090928810693	38.0	35.0	40.0	30.0	41.0
26	36.46460314502751	38.0	35.0	40.0	30.0	41.0
27	36.3458497817614	38.0	35.0	40.0	30.0	41.0
28	36.172699108830074	38.0	35.0	40.0	30.0	41.0
29	35.95821763234409	38.0	34.0	40.0	29.0	41.0
30	35.731061770075655	38.0	34.0	40.0	28.0	41.0
31	35.479313219321796	38.0	34.0	40.0	27.0	41.0
32	35.365850699489826	38.0	34.0	40.0	27.0	41.0
33	35.1772286548187	38.0	33.0	40.0	27.0	41.0
34	35.313305185026564	38.0	34.0	40.0	27.0	41.0
35	35.21512771025367	38.0	34.0	40.0	26.0	41.0
36	35.15575520011347	38.0	34.0	40.0	26.0	41.0
37	35.034806936358315	38.0	33.0	40.0	25.0	41.0
38	34.992259099764034	38.0	33.0	40.0	25.0	41.0
39	34.879006545072286	38.0	33.0	40.0	24.0	41.0
40	34.67184117180015	38.0	33.0	40.0	24.0	41.0
41	34.54285652706534	38.0	33.0	40.0	23.0	41.0
42	34.351627646986024	38.0	33.0	40.0	23.0	41.0
43	33.99130799939374	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	10.0
16	33.0
17	93.0
18	214.0
19	488.0
20	1191.0
21	2306.0
22	4284.0
23	7498.0
24	12500.0
25	19494.0
26	29461.0
27	42378.0
28	59196.0
29	78802.0
30	100869.0
31	122768.0
32	147766.0
33	177563.0
34	212497.0
35	253338.0
36	311213.0
37	395609.0
38	467166.0
39	429926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65543886190551	22.275424206060624	14.251453417634568	22.8176835143993
2	18.214649726697694	23.756756080298455	36.13221963744165	21.896374555562197
3	19.677314170422168	25.031077621748494	33.737052729060146	21.55455547876919
4	13.104258120853709	16.052495456549035	36.759820737047164	34.08342568555009
5	11.962416239899772	38.72671437927491	35.66967060501941	13.641198775805899
6	31.880981746937774	37.04038839379449	13.35416530513775	17.724464554129987
7	24.889142577454194	34.278025827102745	21.63603863914779	19.19679295629527
8	26.523151090080603	34.25397021832203	19.409400041993027	19.813478649604335
9	23.793917129123	14.581696601762872	23.2585755464308	38.36581072268332
10	15.829146776756996	27.415607223357025	34.5793466607895	22.175899339096482
11	31.940668857163914	22.269340778984574	25.00045191172565	20.789538452125864
12	21.369410721014727	26.891459146484753	31.608652788573448	20.130477343927073
13	31.808988732797804	21.00127647681276	27.65011464652855	19.539620143860883
14	20.154672002469525	23.299803800786187	28.623984415302704	27.921539781441584
15	21.51402247322249	29.715420757626532	26.898759258976007	21.871797510174968
16	21.228831411897374	28.883451270706246	27.689917640826124	22.197799676570256
17	21.002180300264055	26.600149895643156	27.44943107789985	24.948238726192944
18	23.021460940226678	24.97803013764536	31.125663441175693	20.874845480952267
19	21.916953920299456	24.216802217009402	32.491584013170794	21.37465984952035
20	23.397868645252075	23.629595073188842	32.5419895518009	20.430546729758177
21	22.414369680477552	24.50376616279668	33.163681036532545	19.918183120193223
22	20.213003377518714	25.019188867119873	32.566392784985965	22.20141497037545
23	21.95359353251748	25.28519106132512	33.70486270921775	19.056352696939655
24	21.548506814133574	25.00816917350212	30.867065646783015	22.57625836558129
25	21.402469801867994	25.908029706591094	31.98022851437844	20.709271977162466
26	21.239816343074697	26.5352136569114	31.795500905909197	20.429469094104707
27	19.46873952781482	26.92385774096976	31.619463907548596	21.987938823666823
28	20.731519939040584	25.854634598083614	31.483959914734687	21.92988554814111
29	20.6514620387198	26.02646534115164	30.49663708151236	22.825435538616205
30	20.930152523683653	26.418203282408676	32.65590606910495	19.995738124802724
31	22.4318204255757	25.82300077728817	32.20023999988876	19.544938797247372
32	21.629259963263053	25.92940860745835	30.686718105808524	21.75461332347007
33	21.120894034348073	26.029107286624665	31.516115172136654	21.33388350689061
34	19.98398841993584	27.166847199607325	32.23743581115374	20.611728569303096
35	19.33194932470483	29.977251458979627	30.596857197285193	20.09394201903035
36	22.37731291897431	26.89928069558253	30.28799291402414	20.435413471419018
37	21.441403735154697	28.024436605127878	30.03241945194927	20.501740207768155
38	21.19292181092848	26.143892864939577	31.512777977854935	21.15040734627701
39	20.812655475014843	25.646789966725393	32.300877264946806	21.23967729331296
40	18.847847579213173	26.687229808931722	31.763693272911574	22.701229338943527
41	21.393709666878486	25.42684800609594	31.71537348070754	21.464068846318032
42	19.325935422509655	28.20770419109887	31.07345025564299	21.39291013074849
43	19.854289754674507	28.44169017766388	29.70071624532271	22.0033038223389
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	306.0
2	403.0
3	1131.0
4	1859.0
5	1859.0
6	3218.0
7	4577.0
8	5127.0
9	5677.0
10	8726.5
11	11776.0
12	11776.0
13	22219.5
14	32663.0
15	54801.5
16	76940.0
17	75235.5
18	73531.0
19	73531.0
20	77656.5
21	81782.0
22	66516.0
23	51250.0
24	56361.5
25	61473.0
26	61473.0
27	66440.0
28	71407.0
29	74573.0
30	77739.0
31	83310.5
32	88882.0
33	88882.0
34	97262.0
35	105642.0
36	116278.0
37	126914.0
38	132228.0
39	137542.0
40	137542.0
41	142024.5
42	146507.0
43	152643.0
44	158779.0
45	172881.0
46	186983.0
47	186983.0
48	261607.0
49	336231.0
50	310400.5
51	284570.0
52	255644.0
53	226718.0
54	226718.0
55	193919.5
56	161121.0
57	132096.0
58	103071.0
59	90256.5
60	77442.0
61	77442.0
62	68168.0
63	58894.0
64	51503.0
65	44112.0
66	37255.5
67	30399.0
68	30399.0
69	25518.0
70	20637.0
71	16850.5
72	13064.0
73	10602.0
74	8140.0
75	8140.0
76	6384.5
77	4629.0
78	3608.5
79	2588.0
80	1953.5
81	1319.0
82	1319.0
83	984.0
84	649.0
85	473.5
86	298.0
87	232.0
88	166.0
89	166.0
90	114.0
91	62.0
92	39.0
93	16.0
94	11.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2876668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.83109448678407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76554969469777	29.710970349669864
2	14.195136168441453	11.592058922927977
3	5.308243075595847	6.502241237354138
4	2.5672662956379693	4.192971707597201
5	1.3670588086763449	2.7909253693027147
6	0.8542709747778163	2.092849133308208
7	0.5285804165280731	1.5107761851785
8	0.35973034513056906	1.1750546969431765
9	0.27199840508349205	0.9995393320396766
>10	1.5143626264192012	11.620707108698468
>50	0.1502838748924596	4.285243426000809
>100	0.10244577773176106	7.819124608848534
>500	0.0071941878211624994	2.0004840315190777
>1k	0.006594672169397443	6.003725133434071
>5k	4.282254655452885E-4	1.0931568511218297
>10k+	8.56450931090577E-4	6.610171906055784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37896	1.317357442708022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36430	1.2663957050309596	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32742	1.1381918247083085	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14492	0.5037772867776191	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12836	0.4462106854179905	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12605	0.43818056167760755	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10810	0.3757819810975754	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10590	0.36813424420197255	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10532	0.3661180226567682	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10092	0.3508225488655625	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8057	0.2800809825812363	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6208	0.215805230217738	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6083	0.21145992516341822	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5676	0.197311611906553	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5236	0.18201613811534734	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4944	0.1718655055084563	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4811	0.16724210093066005	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4699	0.16334870760198952	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4693	0.16314013295938218	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4632	0.1610196240928741	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4056	0.14099645840256853	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4054	0.1409269335216994	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3673	0.1276824437161327	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3649	0.12684814514570328	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3119	0.1084240517153874	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3074	0.10685974189583226	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3041	0.10571258136149184	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2990	0.10393969689932936	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2951	0.10258396172238159	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2936	0.10206252511586321	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2928	0.10178442559238676	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2897	0.10070678993891545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.4762440434558314E-5	0.0	3.4762440434558314E-5	0.0
3	0.0	3.4762440434558314E-5	0.0	3.4762440434558314E-5	0.0
4	0.0	6.952488086911663E-5	0.0	6.952488086911663E-5	0.0
5	0.0	2.780995234764665E-4	0.0	6.952488086911663E-5	0.0
6	6.952488086911663E-5	2.780995234764665E-4	0.0	6.952488086911663E-5	0.0
7	6.952488086911663E-5	2.780995234764665E-4	0.0	6.952488086911663E-5	0.0
8	6.952488086911663E-5	2.780995234764665E-4	0.0	6.952488086911663E-5	0.0
9	6.952488086911663E-5	2.780995234764665E-4	0.0	6.952488086911663E-5	0.0
10	1.0428732130367495E-4	2.780995234764665E-4	0.0	2.4333708304190822E-4	0.0
11	1.0428732130367495E-4	3.1286196391102484E-4	0.0	3.823868447801415E-4	0.0
12	1.0428732130367495E-4	3.1286196391102484E-4	0.0	6.952488086911663E-4	0.0
13	1.0428732130367495E-4	3.1286196391102484E-4	0.0	7.64773689560283E-4	0.0
14	1.3904976173823326E-4	3.1286196391102484E-4	0.0	0.0010776356534713078	0.0
15	2.085746426073499E-4	3.1286196391102484E-4	0.0	0.001320972736513216	0.0
16	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.0021552713069426157	0.0
17	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.0031633820795448067	0.0
18	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.003615293805194065	0.0
19	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.004414829935188906	0.0
20	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.006292001718655055	0.0
21	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.01258400343731011	0.0
22	2.4333708304190822E-4	3.1286196391102484E-4	0.0	0.020370790094651172	0.0
23	2.4333708304190822E-4	4.171492852146998E-4	0.0	0.025480868838531245	0.0
24	2.4333708304190822E-4	4.519117256492581E-4	0.0	0.031320958831537044	0.0
25	2.4333708304190822E-4	4.519117256492581E-4	0.0	0.03570102632629139	0.0
26	2.4333708304190822E-4	4.8667416608381644E-4	0.0	0.04355733786450157	0.0
27	2.4333708304190822E-4	4.8667416608381644E-4	0.0	0.1011587016645647	0.0
28	2.4333708304190822E-4	4.8667416608381644E-4	0.0	0.17482031294539377	0.0
29	2.4333708304190822E-4	4.8667416608381644E-4	0.0	0.26763602890566446	0.0
30	2.4333708304190822E-4	5.214366065183747E-4	0.0	0.4236498615759622	0.0
31	2.4333708304190822E-4	5.214366065183747E-4	0.0	0.6212743354464262	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9300	0.0	29.52043	1
GTATTGG	1135	0.0	22.65639	1
ACGTTTA	170	0.0	20.676472	26
ATTGGAC	1260	0.0	20.261904	3
TTGGACC	2050	0.0	18.680489	4
TCACGTT	250	0.0	17.76	24
GGACCCT	2105	0.0	17.401424	6
TATTGGA	1510	0.0	17.152319	2
TGGACCC	2195	0.0	17.109339	5
GTATCAA	16255	0.0	16.980621	2
CCGACCG	230	0.0	16.891304	9
GACCCTC	2225	0.0	15.964045	7
ATTAGAC	200	3.6379788E-12	15.724999	3
TATACTG	450	0.0	15.622222	5
TTTTTAC	2155	0.0	15.538283	1
CGACGAG	110	1.4532976E-5	15.136364	24
GACGTCG	110	1.4532976E-5	15.136364	36
GATACTG	1040	0.0	15.120193	36
TATACCG	210	9.094947E-12	14.97619	5
TCGAACC	470	0.0	14.957446	31
>>END_MODULE