FastQCFastQC Report
Wed 25 May 2016
SRR2088295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454335
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT496401.437034914100688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT491931.4240946520820938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT439261.2716195736661324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169650.4911220249338874No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA162010.46900488806094376No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG159850.46275187554189157No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG152190.44057684040488254No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA149860.4338316926412754No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG147460.42688390095343964No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA137210.3972110406199746No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA87200.25243643132469784No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA87140.2522627365325019No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA76370.2210845213333391No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC73060.21150235863053235No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA67540.1955224377485102No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA65710.19022474658653546No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG59690.17279736910288088No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59450.1721025899340973No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG56080.16234673243909464No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG54630.15814910829436057No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49270.14263237352486077No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48420.14017169730208565No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT46640.13501875180027414No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC45740.13241332991733573No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG42600.12332330245908403No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40760.11799666216507664No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT39630.11472541024538732No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA38690.11200419183431831No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG38030.1100935491201635No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT37940.10983300693186966No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.104940603618352No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC36100.10450636663786228No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA35660.10323260482842574No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT35610.10308785916826248No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC35000.1013219621142709No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA34900.10103247079394442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119550.031.0731931
GTATTGG12400.024.6169361
ATTGGAC12750.023.070593
TTGGACC21800.019.9426614
GGACCCT22900.018.5807886
TATTGGA16150.018.4427242
TGGACCC23600.017.872885
GACCCTC23100.017.779227
GTATCAA218000.017.1252292
CGTATTA904.450112E-516.44444515
ATTAGAC3650.015.7123293
TTTTTAC25950.015.6127171
ACCGTCG1455.3552867E-815.31034523
CGAATTA2350.014.95744615
ACGTTTA1752.242814E-914.826
ACCCTCG28250.014.6690268
CGTAGAC908.2799396E-414.3888883
GATACTG14450.014.339136
TGATACT14350.014.31010435
TGCGCTA2353.6379788E-1214.17021210
Produced by FastQC (version 0.11.5)