##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3454335
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.915742682745016	31.0	31.0	33.0	28.0	34.0
2	31.221744851035005	31.0	31.0	34.0	28.0	34.0
3	31.27780513470755	31.0	31.0	34.0	28.0	34.0
4	35.17016791944035	35.0	35.0	37.0	33.0	37.0
5	34.934763420455745	35.0	35.0	37.0	32.0	37.0
6	34.9868261184859	36.0	35.0	37.0	32.0	37.0
7	34.7959980719878	35.0	35.0	37.0	32.0	37.0
8	34.86576634866045	35.0	35.0	37.0	32.0	37.0
9	36.32349641826864	38.0	35.0	39.0	32.0	39.0
10	36.136798833928964	38.0	35.0	39.0	31.0	39.0
11	36.23526872755538	38.0	35.0	39.0	32.0	39.0
12	35.982909879904525	38.0	35.0	39.0	30.0	39.0
13	36.07532274663575	38.0	35.0	39.0	31.0	39.0
14	36.87830248079587	39.0	36.0	40.0	31.0	41.0
15	36.99705587327228	38.0	36.0	40.0	31.0	41.0
16	36.96521645989749	38.0	36.0	40.0	31.0	41.0
17	37.068597863264564	39.0	36.0	40.0	31.0	41.0
18	37.08163713131471	39.0	36.0	40.0	31.0	41.0
19	37.17541784453448	39.0	36.0	40.0	31.0	41.0
20	37.156306785531804	39.0	36.0	40.0	31.0	41.0
21	37.086698018576655	39.0	36.0	40.0	31.0	41.0
22	37.01974562397683	39.0	36.0	40.0	31.0	41.0
23	36.90943437738378	39.0	35.0	40.0	31.0	41.0
24	36.82178682727645	39.0	35.0	40.0	30.0	41.0
25	36.69246931753869	38.0	35.0	40.0	30.0	41.0
26	36.44943788022876	38.0	35.0	40.0	30.0	41.0
27	36.317019339467656	38.0	35.0	40.0	30.0	41.0
28	36.14830437696402	38.0	35.0	40.0	30.0	41.0
29	35.92565052318319	38.0	34.0	40.0	29.0	41.0
30	35.70487633654524	38.0	34.0	40.0	28.0	41.0
31	35.43496157726451	38.0	34.0	40.0	27.0	41.0
32	35.322703212050946	38.0	34.0	40.0	27.0	41.0
33	35.123563001272316	37.0	33.0	40.0	27.0	41.0
34	35.2568372783763	38.0	34.0	40.0	27.0	41.0
35	35.14725294448859	38.0	33.0	40.0	26.0	41.0
36	35.062525493329396	38.0	33.0	40.0	25.0	41.0
37	34.933053974209216	38.0	33.0	40.0	25.0	41.0
38	34.87093753211544	38.0	33.0	40.0	25.0	41.0
39	34.744692972742946	38.0	33.0	40.0	24.0	41.0
40	34.525605073045895	38.0	33.0	40.0	23.0	41.0
41	34.38159327337968	38.0	33.0	40.0	23.0	41.0
42	34.193726723088524	38.0	33.0	40.0	22.0	41.0
43	33.82080950457903	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	7.0
15	8.0
16	46.0
17	129.0
18	298.0
19	705.0
20	1502.0
21	2960.0
22	5327.0
23	9550.0
24	15709.0
25	24763.0
26	37057.0
27	52971.0
28	73615.0
29	97328.0
30	123712.0
31	148382.0
32	180439.0
33	215693.0
34	256505.0
35	303406.0
36	371297.0
37	467895.0
38	552162.0
39	512864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.447495682960685	22.656256558787728	14.763941540122774	22.132306218128814
2	18.182139254009815	24.16285623716287	36.318828370728376	21.33617613809894
3	19.601023062326036	25.330606325095857	34.087921408896364	20.98044920368175
4	13.040860252407482	16.288026494245635	36.86509849218446	33.80601476116242
5	11.586542706483304	39.09586070835631	35.66721235780548	13.650384227354904
6	31.12454350837426	37.6530359678491	13.267937244071579	17.954483279705066
7	24.04048825606086	34.96337211069569	21.75124879318306	19.24489084006039
8	26.273016369286704	34.60761622714647	19.474689050135556	19.64467835343127
9	23.56876214958885	14.528266656244979	23.740922637787012	38.162048556379155
10	15.786598578308125	27.66795924541193	34.514892157245896	22.030550019034052
11	31.479401968830466	22.53432281466621	25.27872948049335	20.70754573600997
12	21.079715777421704	27.34566855849245	31.893953539538	19.68066212454785
13	31.62889528664707	21.432547798635625	28.15010703941569	18.788449875301612
14	19.81322020012535	23.665191708389603	29.2296491220452	27.29193896943985
15	20.962240199633214	30.004993725275632	27.733384283805712	21.299381791285445
16	20.463880891691165	29.469087393087236	28.212145029361658	21.85488668585994
17	20.472941970017384	26.799456335300427	27.987297120864074	24.740304573818115
18	22.73511399444466	25.099563302343284	31.86665450803121	20.298668195180838
19	21.7241524056005	24.240526758406467	33.268371481052064	20.766949354940966
20	23.164603317281042	23.48724139378491	33.45046152153743	19.897693767396618
21	21.840730560295977	24.592866644375835	34.112036035879555	19.454366759448636
22	19.49454815470995	25.03662788930431	33.628672378330414	21.840151577655323
23	21.235925293869876	25.371771990846288	35.024860067133034	18.367442648150803
24	21.071031037811906	24.9989361193978	31.820249049382877	22.109783793407413
25	20.737363342003597	26.035836130543217	32.95331807714075	20.273482450312432
26	20.444021787116768	26.914413338602078	32.75753509720395	19.88402977707721
27	18.72250375253124	27.158772962089667	32.46986757219552	21.64885571318358
28	19.922126834832174	26.083833791453348	32.457998428062126	21.536040945652346
29	19.912197282544977	25.989835959743335	31.43345969629466	22.66450706141703
30	20.4201098040578	26.296349369705023	33.72701257984532	19.556528246391853
31	21.85202072178871	25.842079589848698	33.24364892229619	19.06225076606641
32	20.98927868895171	25.985956776050962	31.73241738279582	21.29234715220151
33	20.49135361799015	25.926205767535578	32.746013342654955	20.83642727181932
34	19.177844650272775	26.98777043917281	33.56486849133046	20.269516419223958
35	18.617071013668333	29.896550276681328	31.64279087002274	19.8435878396276
36	21.86328193414941	26.66979317292619	31.28170834617951	20.18521654674489
37	20.79804072274403	27.83305614539412	31.176333505580665	20.19256962628118
38	20.578258912352158	25.737544274078804	32.662871435457184	21.02132537811185
39	20.03048343603038	25.42086971877366	33.532561259982025	21.016085585213943
40	18.24611683580197	26.332101547765348	32.89568035526374	22.526101261168936
41	20.66701695116426	25.20791990354149	32.821657424656266	21.30340572063798
42	18.791171093712684	28.166173807693813	31.980916732164076	21.061738366429427
43	19.39079446550494	28.436442904350624	30.41326333433208	21.75949929581236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	374.0
2	504.0
3	1445.0
4	2386.0
5	2386.0
6	4242.0
7	6098.0
8	6769.0
9	7440.0
10	11655.5
11	15871.0
12	15871.0
13	29800.0
14	43729.0
15	73596.5
16	103464.0
17	101508.5
18	99553.0
19	99553.0
20	104323.5
21	109094.0
22	88648.5
23	68203.0
24	73993.0
25	79783.0
26	79783.0
27	85203.5
28	90624.0
29	93577.0
30	96530.0
31	103595.0
32	110660.0
33	110660.0
34	121054.5
35	131449.0
36	142541.5
37	153634.0
38	159956.5
39	166279.0
40	166279.0
41	171572.5
42	176866.0
43	183283.0
44	189700.0
45	207825.0
46	225950.0
47	225950.0
48	324240.5
49	422531.0
50	381803.5
51	341076.0
52	299593.0
53	258110.0
54	258110.0
55	217720.5
56	177331.0
57	142632.5
58	107934.0
59	94418.0
60	80902.0
61	80902.0
62	70541.5
63	60181.0
64	51942.5
65	43704.0
66	36945.0
67	30186.0
68	30186.0
69	25503.0
70	20820.0
71	17314.5
72	13809.0
73	11266.5
74	8724.0
75	8724.0
76	6898.0
77	5072.0
78	3883.5
79	2695.0
80	2136.0
81	1577.0
82	1577.0
83	1199.5
84	822.0
85	632.0
86	442.0
87	328.5
88	215.0
89	215.0
90	162.5
91	110.0
92	67.0
93	24.0
94	14.0
95	4.0
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3454335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.43649770051816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03101719568852	26.965890096123772
2	14.078820915318124	10.541234936446278
3	5.49457479645634	6.170929101985885
4	2.6796473992752565	4.012664548046704
5	1.5225856712813326	2.850013749088275
6	0.8827866026687186	1.9829063172513435
7	0.604068702001276	1.5829951601398031
8	0.41047689514870317	1.229345387308021
9	0.3053083072490493	1.0286706368050311
>10	1.6710010103958344	11.904558616677544
>50	0.17223940644161828	4.546379951785143
>100	0.12903451054725837	9.229076707621715
>500	0.00988059331686142	2.574584372841909
>1k	0.007001995263855368	6.081857050221821
>5k	7.779994737617073E-4	2.0005484050886486
>10k+	7.779994737617073E-4	7.298344962568226
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	49640	1.437034914100688	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49193	1.4240946520820938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	43926	1.2716195736661324	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16965	0.4911220249338874	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	16201	0.46900488806094376	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15985	0.46275187554189157	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	15219	0.44057684040488254	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14986	0.4338316926412754	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	14746	0.42688390095343964	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13721	0.3972110406199746	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8720	0.25243643132469784	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8714	0.2522627365325019	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7637	0.2210845213333391	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7306	0.21150235863053235	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6754	0.1955224377485102	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6571	0.19022474658653546	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5969	0.17279736910288088	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5945	0.1721025899340973	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5608	0.16234673243909464	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5463	0.15814910829436057	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4927	0.14263237352486077	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4842	0.14017169730208565	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4664	0.13501875180027414	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4574	0.13241332991733573	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4260	0.12332330245908403	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4076	0.11799666216507664	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3963	0.11472541024538732	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3869	0.11200419183431831	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3803	0.1100935491201635	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3794	0.10983300693186966	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3625	0.104940603618352	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3610	0.10450636663786228	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3566	0.10323260482842574	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3561	0.10308785916826248	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3500	0.1013219621142709	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	3490	0.10103247079394442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.7898264065297664E-5	0.0
5	0.0	0.0	0.0	5.7898264065297664E-5	0.0
6	2.8949132032648832E-5	0.0	0.0	5.7898264065297664E-5	0.0
7	2.8949132032648832E-5	0.0	0.0	1.7369479219589299E-4	0.0
8	2.8949132032648832E-5	0.0	0.0	2.026439242285418E-4	0.0
9	2.8949132032648832E-5	0.0	0.0	2.3159305626119066E-4	0.0
10	2.8949132032648832E-5	0.0	0.0	2.894913203264883E-4	0.0
11	2.8949132032648832E-5	0.0	0.0	6.947791687835719E-4	0.0
12	2.8949132032648832E-5	0.0	0.0	0.0011579652813059533	0.0
13	2.8949132032648832E-5	0.0	0.0	0.0014764057336650903	0.0
14	2.8949132032648832E-5	0.0	0.0	0.00173694792195893	0.0
15	2.8949132032648832E-5	0.0	0.0	0.0021711849024486623	0.0
16	8.684739609794649E-5	0.0	0.0	0.0031265062595260736	0.0
17	8.684739609794649E-5	0.0	0.0	0.004255522408799378	0.0
18	8.684739609794649E-5	0.0	0.0	0.00495030157758295	0.0
19	8.684739609794649E-5	0.0	0.0	0.006021419462790957	0.0
20	8.684739609794649E-5	0.0	0.0	0.009813755759067953	0.0
21	8.684739609794649E-5	0.0	0.0	0.017977410992274925	0.0
22	8.684739609794649E-5	0.0	0.0	0.029383369013138563	0.0
23	8.684739609794649E-5	0.0	0.0	0.03636010983300693	0.0
24	8.684739609794649E-5	0.0	0.0	0.04579752687565045	0.0
25	8.684739609794649E-5	0.0	0.0	0.051384709357951676	0.0
26	8.684739609794649E-5	0.0	0.0	0.06125636338108493	0.0
27	8.684739609794649E-5	0.0	0.0	0.136350411873776	0.0
28	8.684739609794649E-5	0.0	0.0	0.23460376599258612	0.0
29	8.684739609794649E-5	0.0	0.0	0.3480264652965043	0.0
30	8.684739609794649E-5	0.0	0.0	0.5460964266638876	0.0
31	8.684739609794649E-5	0.0	0.0	0.8159023372081746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11955	0.0	31.073193	1
GTATTGG	1240	0.0	24.616936	1
ATTGGAC	1275	0.0	23.07059	3
TTGGACC	2180	0.0	19.942661	4
GGACCCT	2290	0.0	18.580788	6
TATTGGA	1615	0.0	18.442724	2
TGGACCC	2360	0.0	17.87288	5
GACCCTC	2310	0.0	17.77922	7
GTATCAA	21800	0.0	17.125229	2
CGTATTA	90	4.450112E-5	16.444445	15
ATTAGAC	365	0.0	15.712329	3
TTTTTAC	2595	0.0	15.612717	1
ACCGTCG	145	5.3552867E-8	15.310345	23
CGAATTA	235	0.0	14.957446	15
ACGTTTA	175	2.242814E-9	14.8	26
ACCCTCG	2825	0.0	14.669026	8
CGTAGAC	90	8.2799396E-4	14.388888	3
GATACTG	1445	0.0	14.3391	36
TGATACT	1435	0.0	14.310104	35
TGCGCTA	235	3.6379788E-12	14.170212	10
>>END_MODULE