FastQCFastQC Report
Wed 25 May 2016
SRR2088294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503900
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT578371.6506464225577215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT572391.6335797254487856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT501771.432032877650618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200500.5722195268129798No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA187500.5351180113587716No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG179780.5130854191044265No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG171550.4895973058591855No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA166250.4744713034047775No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA161250.46020148976854364No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG158780.45315220183224403No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA101700.29024800936099776No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA100870.2878792202973829No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA88490.2525471617340677No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC85000.24258683181597648No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA75770.2162447558434887No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA73160.20879591312537457No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT72130.20585633151631041No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG67790.19347013328005935No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG64870.18513656211649876No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG63320.18071291988926624No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC58140.16592939296212791No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57320.16358914352578555No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT56270.16059248266217643No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC53570.15288678329861013No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG50470.14403949884414508No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47540.13567738805331203No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT44430.12680156397157455No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT44080.12580267701703818No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.12440423528068724No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA43310.12360512571705815No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC42760.12203544621707241No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC42100.12015183081708952No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT40910.11675561517166586No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA40720.11621336225348897No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA40620.11592796598076428No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA40440.11541425268985987No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG40380.11524301492622506No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT39100.11158994263534919No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA38870.11093353120808241No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG37940.10827934587174291No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG37610.10733753817175147No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT37120.10593909643540056No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA36080.1029709751990639No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA35990.10271411855361169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC35780.10211478638088986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142100.030.0999281
GTATTGG14550.023.395191
ATTGGAC16150.021.4210533
TTGGACC25050.018.5369284
TATTGGA19400.017.7371142
GGACCCT24850.017.6438646
TGGACCC26200.017.299625
GTATCAA249400.017.2538092
ACGTTTA2950.016.93220326
TTTTTAC30050.016.0682181
GTACTAT2800.015.8571421
GACCCTC27550.015.8475497
CGTTTAG2500.015.5426
TAGACAG2251.8189894E-1214.85
GATACCC17600.014.50568236
TCTACAC4600.014.4782613
ATACCCT18050.014.24653637
TCACGTT3700.014.024
TTGATAC49950.013.92592634
GTATTAG3600.013.8751
Produced by FastQC (version 0.11.5)