##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3503900
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.926560689517395	31.0	31.0	33.0	28.0	34.0
2	31.232544022375066	31.0	31.0	34.0	28.0	34.0
3	31.278045320928108	31.0	31.0	34.0	28.0	34.0
4	35.173297183138786	35.0	35.0	37.0	33.0	37.0
5	34.951911013442164	35.0	35.0	37.0	32.0	37.0
6	35.01731470646993	36.0	35.0	37.0	32.0	37.0
7	34.80754359428066	35.0	35.0	37.0	32.0	37.0
8	34.88931619053055	36.0	35.0	37.0	32.0	37.0
9	36.32966751334227	38.0	35.0	39.0	32.0	39.0
10	36.15556094637404	38.0	35.0	39.0	31.0	39.0
11	36.25412112217814	38.0	35.0	39.0	32.0	39.0
12	35.995100602186135	38.0	35.0	39.0	30.0	39.0
13	36.08293101972088	38.0	35.0	39.0	31.0	39.0
14	36.85724506977939	38.0	36.0	40.0	31.0	41.0
15	36.98679357287594	38.0	36.0	40.0	31.0	41.0
16	36.95733240103884	38.0	36.0	40.0	31.0	41.0
17	37.084419646679414	39.0	36.0	40.0	31.0	41.0
18	37.10777105511002	39.0	36.0	40.0	31.0	41.0
19	37.20721538856702	39.0	36.0	40.0	31.0	41.0
20	37.19680384714176	39.0	36.0	40.0	31.0	41.0
21	37.1154065469905	39.0	36.0	40.0	31.0	41.0
22	37.04031022574845	39.0	36.0	40.0	31.0	41.0
23	36.90901138731128	39.0	35.0	40.0	31.0	41.0
24	36.82404006963669	39.0	35.0	40.0	30.0	41.0
25	36.69920545677673	38.0	35.0	40.0	30.0	41.0
26	36.44337281315106	38.0	35.0	40.0	30.0	41.0
27	36.31815519849311	38.0	35.0	40.0	30.0	41.0
28	36.13195467907189	38.0	35.0	40.0	30.0	41.0
29	35.900124147378634	38.0	34.0	40.0	29.0	41.0
30	35.64384086303833	38.0	34.0	40.0	28.0	41.0
31	35.339482861953826	38.0	34.0	40.0	27.0	41.0
32	35.22411940980051	37.0	34.0	40.0	27.0	41.0
33	35.011383886526446	37.0	33.0	40.0	27.0	41.0
34	35.10953566026428	37.0	33.0	40.0	27.0	41.0
35	34.982912754359425	37.0	33.0	40.0	26.0	41.0
36	34.89143639944062	38.0	33.0	40.0	25.0	41.0
37	34.739496560974914	37.0	33.0	40.0	25.0	41.0
38	34.648981706098915	38.0	33.0	40.0	24.0	41.0
39	34.50122121065099	38.0	33.0	40.0	23.0	41.0
40	34.258658637518195	38.0	33.0	40.0	22.0	41.0
41	34.084991580809955	37.0	33.0	40.0	21.0	41.0
42	33.85316333228688	37.0	33.0	40.0	20.0	41.0
43	33.4603253517509	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	9.0
15	14.0
16	41.0
17	131.0
18	325.0
19	803.0
20	1637.0
21	3234.0
22	5824.0
23	10342.0
24	16565.0
25	25619.0
26	38372.0
27	55613.0
28	76550.0
29	101396.0
30	128127.0
31	152529.0
32	183350.0
33	220187.0
34	261965.0
35	309116.0
36	379234.0
37	484526.0
38	551522.0
39	496865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8322726105197	22.496903450440936	14.991495191072804	21.67932874796655
2	18.35571791432404	24.17720254573475	36.24920802534319	21.21787151459802
3	19.88875253289192	25.220868175461632	34.246981934415935	20.643397357230516
4	12.946602357373212	16.158623248380376	36.45032677873227	34.44444761551414
5	11.44778675190502	39.198578726561834	35.509346727931735	13.844287793601415
6	31.269414081452098	37.04640543394503	12.914010103028053	18.770170381574815
7	23.831016866919718	35.09620708353549	21.438283056023288	19.634492993521505
8	26.66651445532121	34.22258055309798	19.15448500242587	19.95641998915494
9	23.206969376979934	14.535489026513313	23.94055195639145	38.3169896401153
10	16.023459573617966	27.54113987271326	34.153514655098604	22.281885898570163
11	31.156882331116755	22.265361454379406	25.46488198864123	21.112874225862612
12	21.373926196523875	27.46773595136848	31.685379148948318	19.47295870315934
13	32.40594765832358	21.008504808927196	28.325637147178856	18.259910385570365
14	19.75378863552042	23.7481092496932	29.40255144267816	27.09555067210822
15	20.691172693284628	29.846257027883215	28.16073518079854	21.30183509803362
16	20.28134364565199	29.14064328319872	28.646650874739578	21.931362196409715
17	20.479437198550187	26.419475441650732	27.882844830046523	25.218242529752562
18	23.056936556408573	24.413596278432603	32.25528696595222	20.274180199206597
19	21.596192813721853	23.666542994948486	33.949770256000456	20.787493935329206
20	23.25594337737949	22.820171808556182	34.14834898256229	19.77553583150204
21	21.823910499728875	23.979679785382004	34.99289363280916	19.203516082079968
22	19.270099032506636	24.453837152886784	34.282114215588344	21.993949599018237
23	21.21047975113445	24.932189845600618	35.88019064471018	17.977139758554753
24	20.967892919318473	24.455492451268587	32.42418448015069	22.15243014926225
25	20.55301235765861	25.745683381375038	33.60906418562173	20.092240075344616
26	20.292331402151888	26.57139187762208	33.433145923114246	19.70313079711179
27	18.29421501755187	26.846941978937757	33.14860583920774	21.710237164302633
28	19.663803190730327	25.75284682782043	33.05299808784497	21.53035189360427
29	19.73720711207512	25.730585918547906	31.828990553383374	22.70321641599361
30	20.22620508576158	26.045520705499587	34.513855989040785	19.21441821969805
31	21.82582265475613	25.39561631325095	34.00582208396358	18.77273894802934
32	20.98784211878193	25.63158195153971	32.078826450526556	21.301749479151802
33	20.309654955906275	25.57812722965838	33.186106909443765	20.926110904991578
34	18.811467222238075	26.999372128200005	34.16179114700762	20.027369502554297
35	18.25908273637946	30.194954193898226	32.072718970290246	19.473244099432062
36	21.772367932874797	26.475470190359314	31.72305145694797	20.029110419817915
37	20.715317217957132	27.992522617654615	31.34024943634236	19.951910728045892
38	20.41236907445989	25.522103941322527	33.16755615171666	20.897970832500928
39	19.89280515996461	25.165558377807585	34.03176460515426	20.909871857073547
40	17.819658095265275	26.24181626187962	33.27994520391564	22.658580438939467
41	20.71146436827535	25.002939581609063	33.078912069408375	21.20668398070721
42	18.690230885584636	28.170210337052996	32.19523959017096	20.944319187191414
43	19.247067553297754	28.66066383173036	30.37675161962385	21.715516995348043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	313.0
1	384.0
2	455.0
3	1506.0
4	2557.0
5	2557.0
6	4614.5
7	6672.0
8	7560.0
9	8448.0
10	13436.5
11	18425.0
12	18425.0
13	34757.0
14	51089.0
15	85294.5
16	119500.0
17	115254.0
18	111008.0
19	111008.0
20	115716.0
21	120424.0
22	95251.0
23	70078.0
24	73816.5
25	77555.0
26	77555.0
27	80959.5
28	84364.0
29	86824.5
30	89285.0
31	94765.0
32	100245.0
33	100245.0
34	109020.0
35	117795.0
36	128648.5
37	139502.0
38	145543.5
39	151585.0
40	151585.0
41	157535.5
42	163486.0
43	172143.5
44	180801.0
45	206099.0
46	231397.0
47	231397.0
48	346164.5
49	460932.0
50	416415.0
51	371898.0
52	325908.5
53	279919.0
54	279919.0
55	232901.0
56	185883.0
57	145830.0
58	105777.0
59	90594.0
60	75411.0
61	75411.0
62	65702.5
63	55994.0
64	48472.5
65	40951.0
66	35133.0
67	29315.0
68	29315.0
69	24662.5
70	20010.0
71	16814.5
72	13619.0
73	11176.5
74	8734.0
75	8734.0
76	6911.0
77	5088.0
78	3828.5
79	2569.0
80	2008.0
81	1447.0
82	1447.0
83	1049.5
84	652.0
85	535.0
86	418.0
87	295.5
88	173.0
89	173.0
90	132.0
91	91.0
92	56.5
93	22.0
94	15.0
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3503900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.41739342952501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32974870447465	23.836542756865818
2	13.724575203633826	9.172790584658701
3	5.497121084704059	5.510983740518785
4	2.7863461028343766	3.7244969579696052
5	1.596676370653487	2.6678381228876846
6	0.9875461857266399	1.9800731650952346
7	0.6791464506443025	1.5886712896212307
8	0.49217564045038464	1.3157781610687163
9	0.35696869872670745	1.0736067102638371
>10	2.1563129300062003	13.826272452970564
>50	0.22278899654593196	5.164467304440212
>100	0.14760834775722537	9.322945925494382
>500	0.011966121550788878	2.714183942157786
>1k	0.008866982156323193	6.746550999970355
>5k	0.0011191336702155487	2.4921805135169683
>10k+	0.0010330464648143525	8.862617372500125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	57837	1.6506464225577215	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57239	1.6335797254487856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50177	1.432032877650618	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20050	0.5722195268129798	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	18750	0.5351180113587716	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17978	0.5130854191044265	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	17155	0.4895973058591855	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	16625	0.4744713034047775	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	16125	0.46020148976854364	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	15878	0.45315220183224403	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	10170	0.29024800936099776	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10087	0.2878792202973829	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8849	0.2525471617340677	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8500	0.24258683181597648	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7577	0.2162447558434887	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	7316	0.20879591312537457	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7213	0.20585633151631041	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	6779	0.19347013328005935	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	6487	0.18513656211649876	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	6332	0.18071291988926624	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5814	0.16592939296212791	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5732	0.16358914352578555	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5627	0.16059248266217643	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	5357	0.15288678329861013	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	5047	0.14403949884414508	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4754	0.13567738805331203	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4443	0.12680156397157455	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4408	0.12580267701703818	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4359	0.12440423528068724	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	4331	0.12360512571705815	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	4276	0.12203544621707241	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	4210	0.12015183081708952	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	4091	0.11675561517166586	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	4072	0.11621336225348897	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	4062	0.11592796598076428	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4044	0.11541425268985987	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4038	0.11524301492622506	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3910	0.11158994263534919	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3887	0.11093353120808241	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3794	0.10827934587174291	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3761	0.10733753817175147	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3712	0.10593909643540056	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	3608	0.1029709751990639	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	3599	0.10271411855361169	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3578	0.10211478638088986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8539627272467823E-5	0.0	0.0	0.0	0.0
3	5.7079254544935646E-5	0.0	0.0	0.0	0.0
4	5.7079254544935646E-5	0.0	0.0	2.8539627272467823E-5	0.0
5	8.561888181740346E-5	0.0	0.0	2.8539627272467823E-5	0.0
6	1.1415850908987129E-4	0.0	0.0	2.8539627272467823E-5	0.0
7	1.1415850908987129E-4	0.0	0.0	2.8539627272467823E-5	0.0
8	1.7123776363480692E-4	0.0	0.0	2.8539627272467823E-5	0.0
9	1.7123776363480692E-4	0.0	0.0	8.561888181740346E-5	0.0
10	1.9977739090727475E-4	0.0	0.0	2.853962727246782E-4	0.0
11	1.9977739090727475E-4	0.0	0.0	8.276491909015669E-4	0.0
12	1.9977739090727475E-4	0.0	0.0	0.001426981363623391	0.0
13	1.9977739090727475E-4	0.0	0.0	0.001769456890893005	0.0
14	1.9977739090727475E-4	0.0	0.0	0.0020548531636176834	0.0
15	2.2831701817974259E-4	0.0	0.0	0.0025114871999771683	0.0
16	2.2831701817974259E-4	0.0	0.0	0.0032820571363337994	0.0
17	2.568566454522104E-4	0.0	0.0	0.004766117754502126	0.0
18	2.568566454522104E-4	0.0	0.0	0.0057650047090385	0.0
19	2.853962727246782E-4	0.0	0.0	0.007363223836296698	0.0
20	2.853962727246782E-4	0.0	0.0	0.011415850908987129	0.0
21	2.853962727246782E-4	0.0	0.0	0.020234595736179684	0.0
22	2.853962727246782E-4	0.0	0.0	0.03273495248152059	0.0
23	3.4247552726961384E-4	0.0	0.0	0.040783127372356516	0.0
24	3.4247552726961384E-4	0.0	0.0	0.0502868232540883	0.0
25	3.710151545420817E-4	0.0	0.0	0.056251605354034076	0.0
26	3.710151545420817E-4	0.0	0.0	0.06809555067210822	0.0
27	3.995547818145495E-4	0.0	0.0	0.15368589286223922	0.0
28	3.995547818145495E-4	0.0	0.0	0.2602243214703616	0.0
29	3.995547818145495E-4	0.0	0.0	0.3829732583692457	0.0
30	4.5663403635948517E-4	0.0	0.0	0.6024144524672508	0.0
31	4.85173663631953E-4	0.0	0.0	0.8765661120465766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14210	0.0	30.099928	1
GTATTGG	1455	0.0	23.39519	1
ATTGGAC	1615	0.0	21.421053	3
TTGGACC	2505	0.0	18.536928	4
TATTGGA	1940	0.0	17.737114	2
GGACCCT	2485	0.0	17.643864	6
TGGACCC	2620	0.0	17.29962	5
GTATCAA	24940	0.0	17.253809	2
ACGTTTA	295	0.0	16.932203	26
TTTTTAC	3005	0.0	16.068218	1
GTACTAT	280	0.0	15.857142	1
GACCCTC	2755	0.0	15.847549	7
CGTTTAG	250	0.0	15.54	26
TAGACAG	225	1.8189894E-12	14.8	5
GATACCC	1760	0.0	14.505682	36
TCTACAC	460	0.0	14.478261	3
ATACCCT	1805	0.0	14.246536	37
TCACGTT	370	0.0	14.0	24
TTGATAC	4995	0.0	13.925926	34
GTATTAG	360	0.0	13.875	1
>>END_MODULE