FastQCFastQC Report
Wed 25 May 2016
SRR2088293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230129
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210231.7090077544712792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT208321.693480927610031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165681.3468506148542145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.5706718563662836No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG58480.47539729573077294No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58220.4732836962627497No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57350.4662112672735949No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55200.4487334255187871No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52150.4239392779131294No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG49020.3984947920096185No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA33220.27005297818358887No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30130.24493366142900458No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29970.24363298483329798No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28780.2339592026527299No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.2114412390895589No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25170.20461268696209908No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA24360.19802801169633427No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG21880.17786752446288154No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21260.17282740265451835No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG18990.15437405345293054No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18580.1510410696764323No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.14803325504886075No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC18050.1467325784531541No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG17490.1421802103681809No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG17280.14047307233631595No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16780.13640845797473275No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT16030.1303115364323579No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA15890.12917344441111459No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT15400.12519012233676305No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA15040.12226359999642314No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA14500.11787381648591326No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA14370.11681701675190163No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.11494729414557336No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT14050.11421566356048837No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA13670.11112655664568512No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA13450.1093381263265885No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT13420.10909424946489352No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG13170.1070619422841019No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG13150.10689935770963857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12780.103891543082067No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12610.10250957419912872No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12600.10242828191189704No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT12350.10039597473110544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.030.3731351
ACGGTCG405.940465E-527.7527
ATTGGAC3450.020.9130423
CGGTCGA555.1433756E-420.18181828
GTATTGG3600.019.013891
TCACGTA701.2192858E-418.525
GTATCAA79550.017.9302332
TCCGGTC1054.7979483E-717.6190478
TTGGACC6800.017.1397064
AACGGTC650.001580019417.07692326
TATACTG1201.04135324E-716.9583345
TTTTTAC10000.016.2799991
CCGGTCT1151.2422915E-616.0869569
GTCGAAC700.002592632515.85714230
TACTCCG1059.344087E-615.8571425
ACGTAGG957.060319E-515.57894827
TATTGGA4550.015.450552
ACTCTGT5250.015.15238237
GTATTAT1101.4519537E-515.1363641
GACAGTA750.00410462314.82
Produced by FastQC (version 0.11.5)