##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230129
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.664931889257144	31.0	30.0	31.0	27.0	34.0
2	30.95031984450411	31.0	31.0	34.0	27.0	34.0
3	30.972283394668363	31.0	31.0	34.0	27.0	34.0
4	34.87230444937076	35.0	35.0	37.0	32.0	37.0
5	34.619626884660065	35.0	35.0	37.0	32.0	37.0
6	34.7173792342104	35.0	35.0	37.0	31.0	37.0
7	34.488099215610724	35.0	35.0	37.0	30.0	37.0
8	34.59681545593999	35.0	35.0	37.0	30.0	37.0
9	35.97516764501934	37.0	35.0	39.0	30.0	39.0
10	35.77793954942937	37.0	35.0	39.0	30.0	39.0
11	35.907261758726115	37.0	35.0	39.0	30.0	39.0
12	35.647137820505	37.0	35.0	39.0	30.0	39.0
13	35.731870397332315	37.0	35.0	39.0	30.0	39.0
14	36.414919085721905	38.0	35.0	40.0	30.0	41.0
15	36.54319425035911	38.0	35.0	40.0	30.0	41.0
16	36.483689921951274	38.0	35.0	40.0	30.0	41.0
17	36.63005912388051	38.0	35.0	40.0	30.0	41.0
18	36.64371378936681	38.0	35.0	40.0	30.0	41.0
19	36.758540770927276	39.0	35.0	40.0	30.0	41.0
20	36.74779067886376	39.0	35.0	40.0	30.0	41.0
21	36.66832421640332	38.0	35.0	40.0	30.0	41.0
22	36.594407578392186	38.0	35.0	40.0	30.0	41.0
23	36.448233477952314	38.0	35.0	40.0	30.0	41.0
24	36.3686605225956	38.0	35.0	40.0	30.0	41.0
25	36.21679840081813	38.0	34.0	40.0	30.0	41.0
26	35.925341163406436	38.0	34.0	40.0	29.0	41.0
27	35.77998974091335	38.0	34.0	40.0	29.0	41.0
28	35.58481427557598	38.0	34.0	40.0	27.0	41.0
29	35.36723546880042	38.0	34.0	40.0	27.0	41.0
30	35.098275871880105	37.0	33.0	40.0	27.0	41.0
31	34.763807698217015	37.0	33.0	40.0	25.0	41.0
32	34.64463483098114	37.0	33.0	40.0	25.0	41.0
33	34.45670494720473	37.0	33.0	40.0	25.0	41.0
34	34.54433884576333	37.0	33.0	40.0	25.0	41.0
35	34.42890704958586	37.0	33.0	40.0	25.0	41.0
36	34.32866553020049	37.0	33.0	40.0	24.0	41.0
37	34.17865280795754	37.0	33.0	40.0	24.0	41.0
38	34.0891052889575	37.0	33.0	40.0	23.0	41.0
39	33.92106193740656	37.0	33.0	40.0	22.0	41.0
40	33.67023214638465	37.0	32.0	40.0	21.0	41.0
41	33.50061497615291	37.0	32.0	40.0	19.0	41.0
42	33.24818697876402	37.0	31.0	40.0	18.0	41.0
43	32.843953764198716	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	7.0
16	30.0
17	75.0
18	184.0
19	418.0
20	854.0
21	1590.0
22	2845.0
23	4862.0
24	7805.0
25	11821.0
26	17143.0
27	23914.0
28	31861.0
29	41237.0
30	50741.0
31	60699.0
32	71110.0
33	82960.0
34	97614.0
35	112915.0
36	136797.0
37	168205.0
38	184088.0
39	120353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44748884060127	23.849775104887375	15.460817524015773	21.24191853049558
2	19.16969683667323	25.31246722904671	34.71481446254824	20.803021471731824
3	20.49654954886845	25.560571289677746	34.201047207244116	19.74183195420968
4	13.950000365815294	17.300787153217264	35.93761304708693	32.81159943388051
5	11.312553398871176	39.967027848298834	35.2428078681179	13.47761088471209
6	31.084463499356573	37.67938159331257	13.03302336584212	18.203131541488737
7	23.25170774772402	35.547084899226014	21.692765555482392	19.50844179756757
8	26.739959792834732	34.65018709419906	19.393331918847537	19.216521194118666
9	23.409658661815143	14.465393466864043	23.788805889463628	38.33614198185719
10	15.886870401396926	28.436367242785103	34.01318073145174	21.663581624366223
11	31.384675916103106	22.442524320620034	25.28173874447314	20.89106101880372
12	20.82854724992257	28.026654115137518	31.962176324596854	19.18262231034306
13	32.22865244214225	21.327031555227137	28.355806586138527	18.08850941649209
14	19.572174950757194	23.788318135740237	29.800370530245203	26.83913638325737
15	20.76164369753091	30.406973577567882	27.874149784290914	20.957232940610293
16	19.997577489840495	29.157998876540592	28.995089132928335	21.849334500690578
17	20.390137944882202	26.991965883252895	28.36978885954237	24.248107312322528
18	22.455287209715404	24.93006830990896	32.471472504103225	20.143171976272406
19	21.18956629751839	24.121535221102828	34.32225400750653	20.366644473872253
20	22.627870735508225	23.4911948259085	34.3093285338367	19.571605904746576
21	21.52757962782765	24.307857143437804	35.29231487104198	18.872248357692566
22	19.07117058454845	24.94632676735529	34.41996733675899	21.562535311337268
23	20.965280877046226	25.281413575324212	35.73714626677364	18.01615928085591
24	20.690838115352133	24.941611814695857	32.71721908840455	21.650330981547462
25	20.224139094355145	26.082223896843338	33.81921733411699	19.874419674684525
26	20.149187605527548	26.944816356658528	33.53802731258266	19.367968725231254
27	18.27361195451859	27.211780227927314	33.27000664157987	21.244601175974225
28	19.578434456874035	26.36959213220727	33.155953562593844	20.89601984832485
29	19.72858131139092	25.909071325039896	31.99046604055347	22.371881323015717
30	20.04464572414763	26.464622815981087	34.5296306322345	18.961100827636777
31	21.413770425703323	25.996948287537325	34.08821351256657	18.50106777419279
32	20.664499414289068	26.15506178620291	32.380099973254836	20.80033882625318
33	20.189833749143382	26.14920874152223	33.143515842647396	20.517441666686988
34	18.736408945728456	27.55686598722573	34.06024896575887	19.64647610128694
35	18.186547914893477	30.4013644097489	32.300677408629504	19.111410266728125
36	21.569770324900883	27.031799103996413	31.79690910465488	19.601521466447828
37	20.386804961105707	28.7369861209678	31.32899069934942	19.547218218577076
38	20.27860492680036	26.318703160400254	32.99255606525819	20.41013584754119
39	19.774592745964043	25.997842502696873	33.90262322081668	20.32494153052241
40	17.851786276073486	26.91018584229784	33.0392991304164	22.198728751212272
41	20.452895590625047	25.762745208022896	32.93809023281298	20.84626896853907
42	18.756732017536372	28.65992103267218	31.95120186582058	20.63214508397087
43	19.242778602894493	29.07093483691548	30.327551012942543	21.358735547247484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	157.0
2	209.0
3	468.0
4	727.0
5	727.0
6	1379.5
7	2032.0
8	2359.5
9	2687.0
10	4339.5
11	5992.0
12	5992.0
13	11792.0
14	17592.0
15	30374.0
16	43156.0
17	41074.0
18	38992.0
19	38992.0
20	41679.0
21	44366.0
22	35202.5
23	26039.0
24	27693.5
25	29348.0
26	29348.0
27	31049.0
28	32750.0
29	33484.0
30	34218.0
31	36716.0
32	39214.0
33	39214.0
34	42311.5
35	45409.0
36	49640.0
37	53871.0
38	55791.0
39	57711.0
40	57711.0
41	59634.5
42	61558.0
43	63725.5
44	65893.0
45	72819.5
46	79746.0
47	79746.0
48	116090.5
49	152435.0
50	137391.0
51	122347.0
52	106835.5
53	91324.0
54	91324.0
55	76456.5
56	61589.0
57	49114.5
58	36640.0
59	31296.0
60	25952.0
61	25952.0
62	22372.5
63	18793.0
64	16143.5
65	13494.0
66	11376.0
67	9258.0
68	9258.0
69	7732.5
70	6207.0
71	5250.5
72	4294.0
73	3553.5
74	2813.0
75	2813.0
76	2202.5
77	1592.0
78	1221.0
79	850.0
80	653.0
81	456.0
82	456.0
83	351.0
84	246.0
85	185.5
86	125.0
87	91.5
88	58.0
89	58.0
90	44.5
91	31.0
92	19.5
93	8.0
94	4.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1230129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.28968103630022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33942386371731	35.47967519355283
2	11.963836198850112	10.83676650832928
3	4.001897388731755	5.437339688269917
4	1.70949004107214	3.0968903477955596
5	0.9391235183151625	2.1266302299090882
6	0.5807042905858915	1.5779947258227611
7	0.4018664520036831	1.2740282401305523
8	0.2806375819114275	1.0167989257253705
9	0.20744690094265145	0.8455683578095486
>10	1.3539200017770925	11.762057366282736
>50	0.1322095434871533	4.116842369324898
>100	0.0726734288081907	6.191915055580955
>500	0.007393573996673516	2.459043213439956
>1k	0.007754236142852712	6.135398164112053
>5k	0.0010819864385375878	2.87156529225643
>10k+	5.409932192687939E-4	4.771486321658061
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21023	1.7090077544712792	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20832	1.693480927610031	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16568	1.3468506148542145	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7020	0.5706718563662836	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5848	0.47539729573077294	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5822	0.4732836962627497	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5735	0.4662112672735949	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5520	0.4487334255187871	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5215	0.4239392779131294	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4902	0.3984947920096185	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3322	0.27005297818358887	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3013	0.24493366142900458	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2997	0.24363298483329798	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2878	0.2339592026527299	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2601	0.2114412390895589	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2517	0.20461268696209908	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2436	0.19802801169633427	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2188	0.17786752446288154	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2126	0.17282740265451835	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1899	0.15437405345293054	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1858	0.1510410696764323	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.14803325504886075	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1805	0.1467325784531541	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1749	0.1421802103681809	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1728	0.14047307233631595	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1678	0.13640845797473275	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1603	0.1303115364323579	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1589	0.12917344441111459	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1540	0.12519012233676305	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1504	0.12226359999642314	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1450	0.11787381648591326	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1437	0.11681701675190163	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1414	0.11494729414557336	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1405	0.11421566356048837	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1367	0.11112655664568512	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1345	0.1093381263265885	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1342	0.10909424946489352	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1317	0.1070619422841019	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1315	0.10689935770963857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1278	0.103891543082067	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1261	0.10250957419912872	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1260	0.10242828191189704	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1235	0.10039597473110544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.129228723166432E-5	0.0	0.0	0.0	0.0
3	8.129228723166432E-5	0.0	0.0	0.0	0.0
4	8.129228723166432E-5	0.0	0.0	8.129228723166432E-5	0.0
5	1.6258457446332865E-4	0.0	0.0	8.129228723166432E-5	0.0
6	3.251691489266573E-4	0.0	0.0	8.129228723166432E-5	0.0
7	4.064614361583216E-4	0.0	0.0	8.129228723166432E-5	0.0
8	4.8775372338998594E-4	0.0	0.0	8.129228723166432E-5	0.0
9	4.8775372338998594E-4	0.0	0.0	1.6258457446332865E-4	0.0
10	4.8775372338998594E-4	0.0	0.0	3.251691489266573E-4	0.0
11	4.8775372338998594E-4	0.0	0.0	3.251691489266573E-4	0.0
12	4.8775372338998594E-4	0.0	0.0	4.064614361583216E-4	0.0
13	4.8775372338998594E-4	0.0	0.0	6.503382978533146E-4	0.0
14	4.8775372338998594E-4	0.0	0.0	6.503382978533146E-4	0.0
15	4.8775372338998594E-4	0.0	0.0	8.942151595483076E-4	0.0
16	4.8775372338998594E-4	0.0	0.0	0.0016258457446332863	0.0
17	4.8775372338998594E-4	0.0	0.0	0.0021948917552549367	0.0
18	4.8775372338998594E-4	0.0	0.0	0.0029265223403399155	0.0
19	4.8775372338998594E-4	0.0	0.0	0.0037394452126565587	0.0
20	4.8775372338998594E-4	0.0	0.0	0.005690460106216502	0.0
21	4.8775372338998594E-4	0.0	0.0	0.009511197606104726	0.0
22	4.8775372338998594E-4	0.0	0.0	0.016095872871869536	0.0
23	4.8775372338998594E-4	0.0	0.0	0.019997902658989423	0.0
24	4.8775372338998594E-4	0.0	0.0	0.025281901329047604	0.0
25	4.8775372338998594E-4	0.0	0.0	0.029590392552325814	0.0
26	4.8775372338998594E-4	0.0	0.0	0.03771962127549224	0.0
27	4.8775372338998594E-4	0.0	0.0	0.0982010829758505	0.0
28	4.8775372338998594E-4	0.0	0.0	0.17209577206943336	0.0
29	4.8775372338998594E-4	0.0	0.0	0.265907071534774	0.0
30	4.8775372338998594E-4	0.0	0.0	0.4342633983915508	0.0
31	4.8775372338998594E-4	0.0	0.0	0.6286332571624602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4690	0.0	30.373135	1
ACGGTCG	40	5.940465E-5	27.75	27
ATTGGAC	345	0.0	20.913042	3
CGGTCGA	55	5.1433756E-4	20.181818	28
GTATTGG	360	0.0	19.01389	1
TCACGTA	70	1.2192858E-4	18.5	25
GTATCAA	7955	0.0	17.930233	2
TCCGGTC	105	4.7979483E-7	17.619047	8
TTGGACC	680	0.0	17.139706	4
AACGGTC	65	0.0015800194	17.076923	26
TATACTG	120	1.04135324E-7	16.958334	5
TTTTTAC	1000	0.0	16.279999	1
CCGGTCT	115	1.2422915E-6	16.086956	9
GTCGAAC	70	0.0025926325	15.857142	30
TACTCCG	105	9.344087E-6	15.857142	5
ACGTAGG	95	7.060319E-5	15.578948	27
TATTGGA	455	0.0	15.45055	2
ACTCTGT	525	0.0	15.152382	37
GTATTAT	110	1.4519537E-5	15.136364	1
GACAGTA	75	0.004104623	14.8	2
>>END_MODULE