FastQCFastQC Report
Wed 25 May 2016
SRR2088292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696878
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159042.282178516182172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131461.8864134037808626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83521.1984881141318853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.766992213845178No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA40100.5754235318090111No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32290.4633522653893508No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29260.41987263193844543No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21470.3080883597989892No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.2716400862130818No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA18550.2661871948892059No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17400.24968502377747614No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.22041160719666855No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13710.19673457907983896No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12500.1793714251274972No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12440.17851044228688523No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT12100.1736315395234173No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.16430422541678744No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT11400.16358673971627746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11020.15813384839240155No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA10890.15626838557107559No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC10880.15612488843097358No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9670.13876173447863185No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA8780.12599048900955404No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG8330.11953311770496414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8300.11910262628465815No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.11781115202374016No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7760.11135378071915027No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7630.10948831789782429No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.1090578264775183No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7560.1084838379171103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC7460.10704886651609034No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG7210.1034614380135404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.031.855341
TGCACCC250.005494876329.619
TAGTACC400.001930428323.1254
TAACACT652.68033E-622.769234
GTATCAA53950.021.054682
ATCGCTC555.1403005E-420.1818188
TATAGTG656.897149E-519.9230775
ATACCCT2000.019.42500137
GATACCC1850.019.036
CATATAC1306.9485395E-1018.53
GGACTAG500.0070321718.51
CGGGATT609.231839E-418.518
TATACTG1002.8708928E-718.55
TGATACC4200.018.05952535
GTGGTAT3900.018.025641
TTGATAC6500.017.64615434
ATAGTAC852.7206248E-517.4117643
ACCGTGG752.0657953E-417.2666668
GTATACT650.001579090517.0769234
AGGTCGC650.001579090517.07692335
Produced by FastQC (version 0.11.5)