FastQCFastQC Report
Wed 25 May 2016
SRR2088291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872482
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT326831.7454373393175473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT315921.6871724267576402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT256561.3701600335810973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112010.598189995951897No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95340.5091637730028914No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA89380.47733436155861575No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG86400.461419655836478No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA86120.45992431435923015No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82560.440912115577079No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74330.3969597571565441No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA46430.2479596599593481No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44140.23572990287757103No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC42600.22750552475270788No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA41350.22082989315785145No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39540.21116357860849932No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38450.20534242785778448No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37670.20117683374259407No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33450.17863990147835865No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30910.16507501807761035No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29880.15957429764344866No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29540.1577585258496477No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28510.15225780541548598No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27970.14937393256650797No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC27680.14782518603650127No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.14686389508684194No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG24120.12881298725435011No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA23990.12811872156848506No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23370.12480760829743623No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT21710.11594236953946686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC21670.11572874932843145No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC21340.11396638258738935No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA21190.11316530679600657No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT21050.11241763605738266No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT20590.10996100363047548No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT20170.10771799141460373No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA19860.10606243477907931No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA19230.10269791645527167No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA19230.10269791645527167No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG18990.10141619518905924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78850.030.2897911
ACGTTTA1400.022.46428526
GTATTGG6300.022.317461
TACGGAT603.7278987E-521.5833347
CGAATTA851.2461642E-619.58823615
TCACGTT1650.019.06060624
ATTGGAC7350.018.625853
GTTATAC701.2197173E-418.53
GTATCAA132050.018.1287382
CGTTTAT1850.018.027
GATACCT7250.017.8620736
GGACCCT12450.017.8313266
TTGGACC12650.017.6956524
TGGACCC13200.017.518945
GGTATCG650.001580435917.07692335
TAACACT2000.016.6500024
TAAGCCG2050.016.24390236
CGGTTTA803.3839874E-416.187515
TAGTGCC700.002593310515.8571427
GACCCTC14000.015.5928577
Produced by FastQC (version 0.11.5)