##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1872482
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.673967493412487	31.0	30.0	31.0	28.0	34.0
2	30.939336666520692	31.0	31.0	34.0	27.0	34.0
3	30.962377208432443	31.0	31.0	34.0	27.0	34.0
4	34.85042793468776	35.0	35.0	37.0	32.0	37.0
5	34.607996765790006	35.0	35.0	37.0	32.0	37.0
6	34.708604942530826	35.0	35.0	37.0	31.0	37.0
7	34.48534885782614	35.0	35.0	37.0	30.0	37.0
8	34.59326818628964	35.0	35.0	37.0	30.0	37.0
9	35.9739212446368	37.0	35.0	39.0	30.0	39.0
10	35.770644524219726	37.0	35.0	39.0	30.0	39.0
11	35.898308768789235	37.0	35.0	39.0	30.0	39.0
12	35.63810546643439	37.0	35.0	39.0	30.0	39.0
13	35.73495980201679	37.0	35.0	39.0	30.0	39.0
14	36.44696130590307	38.0	35.0	40.0	30.0	41.0
15	36.5627925929328	38.0	35.0	40.0	30.0	41.0
16	36.49968811449189	38.0	35.0	40.0	30.0	41.0
17	36.631272823984425	38.0	35.0	40.0	30.0	41.0
18	36.65651044976667	38.0	35.0	40.0	30.0	41.0
19	36.763725365584285	39.0	35.0	40.0	30.0	41.0
20	36.74507738926195	39.0	35.0	40.0	30.0	41.0
21	36.668862504419266	38.0	35.0	40.0	30.0	41.0
22	36.586353834108955	38.0	35.0	40.0	30.0	41.0
23	36.455481548020224	38.0	35.0	40.0	30.0	41.0
24	36.364771997808255	38.0	35.0	40.0	30.0	41.0
25	36.23297153190258	38.0	34.0	40.0	30.0	41.0
26	35.931574242102194	38.0	34.0	40.0	29.0	41.0
27	35.77153318429763	38.0	34.0	40.0	29.0	41.0
28	35.57950143178947	38.0	34.0	40.0	27.0	41.0
29	35.35011231082595	38.0	34.0	40.0	27.0	41.0
30	35.08973010154437	37.0	33.0	40.0	27.0	41.0
31	34.76668720981029	37.0	33.0	40.0	25.0	41.0
32	34.647651085564505	37.0	33.0	40.0	25.0	41.0
33	34.444039515466635	37.0	33.0	40.0	25.0	41.0
34	34.53902574230353	37.0	33.0	40.0	25.0	41.0
35	34.42006385108108	37.0	33.0	40.0	25.0	41.0
36	34.317096239109375	37.0	33.0	40.0	24.0	41.0
37	34.15767948637156	37.0	33.0	40.0	24.0	41.0
38	34.061057996819194	37.0	33.0	40.0	23.0	41.0
39	33.885901706932295	37.0	33.0	40.0	22.0	41.0
40	33.64819474900159	37.0	32.0	40.0	21.0	41.0
41	33.468524664055515	37.0	32.0	40.0	19.0	41.0
42	33.233502378127	37.0	31.0	40.0	18.0	41.0
43	32.82377080260318	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	8.0
15	12.0
16	47.0
17	100.0
18	283.0
19	652.0
20	1318.0
21	2608.0
22	4497.0
23	7506.0
24	11859.0
25	18056.0
26	26416.0
27	36519.0
28	49004.0
29	63213.0
30	77565.0
31	91128.0
32	107720.0
33	126476.0
34	147695.0
35	171916.0
36	208194.0
37	255609.0
38	280657.0
39	183421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11915735371555	23.52070674110619	15.254672675091136	21.105463230087125
2	19.447289746977543	25.038104505143437	34.60658099784137	20.90802475003765
3	21.055209075441045	25.57151417209885	33.66446246212247	19.708814290337635
4	14.127559036615573	17.040430829241615	35.70111755413403	33.13089258000878
5	11.344461522193537	40.02783471349791	35.10250031776007	13.525203446548485
6	31.46561622488227	37.24868917298003	12.854008743475237	18.431685858662462
7	23.564231859104652	35.1557985604134	21.72186434902979	19.558105231452156
8	26.861032576014082	34.559370931202544	19.237461294688014	19.342135198095363
9	23.374857542021765	14.311219013053265	23.70116241437835	38.61276103054662
10	16.10968756976035	28.100403635388748	33.98174188056281	21.808166914288094
11	31.41584271571102	22.2842195545805	25.12136298239449	21.178574747313995
12	20.77499276361535	27.853618886590098	31.907810061725563	19.46357828806899
13	32.36517093355237	20.925114366920482	28.444919630736106	18.26479506879105
14	19.6687605007685	23.618651607865925	29.51937588719144	27.19321200417414
15	21.06375388388246	29.90901915212002	28.08454233471937	20.942684629278148
16	20.136909193252592	28.843107704106103	29.02046588431825	21.99951721832306
17	20.42396135183142	26.561483635089683	28.447643288426804	24.566911724652094
18	22.494208222028302	24.535082313207816	32.441326538786484	20.529382925977394
19	21.485546990571873	23.693632301939353	34.210155291212416	20.610665416276362
20	22.92257015020705	23.085829396490862	34.40503032872947	19.586570124572624
21	21.700769353190044	24.02287445219767	35.27216817037493	19.004188024237347
22	19.221973829387945	24.48573604445864	34.543135795163856	21.749154330989565
23	20.932804694517756	25.021121698366127	35.964885109709996	18.081188497406117
24	20.79277664618405	24.59414830155911	32.823012450854	21.790062601402845
25	20.449275346839116	25.6828103020483	33.793756094851645	20.07415825626094
26	20.39410792733922	26.587171465466692	33.60544987882393	19.41327072837015
27	18.503729274834154	26.8210321914977	33.36048090181908	21.314757631849066
28	19.67661104352405	25.879180681042595	33.424994205551776	21.019214069881578
29	19.87111224567179	25.440084337259318	32.28223288661787	22.406570530451027
30	20.172797388706538	25.94129075740114	34.639585320446336	19.24632653344598
31	21.539432688805555	25.533489774534544	34.1781122595571	18.748965277102798
32	20.87277741521681	25.71613505496982	32.35924297269614	21.05184455711724
33	20.34817958196661	25.76489386813865	33.20282918607495	20.684097363819788
34	18.96002204560578	27.116255323148636	34.182117638514015	19.741604992731574
35	18.35494279784799	30.02966116630227	32.324102447980806	19.291293587868935
36	21.724053956192904	26.571256759744553	31.963030886278215	19.74165839778433
37	20.628235678634027	28.16619866038766	31.46000869434259	19.74555696663573
38	20.584176510107973	25.710207094113592	33.12795530210704	20.577661093671395
39	19.90262122679951	25.50128652772096	34.042570235655134	20.553522009824395
40	18.13956022007154	26.49205706650318	32.96346773960978	22.404914973815504
41	20.76009275389563	25.309669198422203	32.83182428455921	21.098413763122956
42	18.9972453673787	28.294103761745106	31.80286913305442	20.90578173782178
43	19.612097739791356	28.560488164906257	30.22656559582415	21.600848499478232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	205.5
2	265.0
3	691.0
4	1117.0
5	1117.0
6	2147.5
7	3178.0
8	3679.5
9	4181.0
10	6896.5
11	9612.0
12	9612.0
13	18603.5
14	27595.0
15	46748.0
16	65901.0
17	62933.0
18	59965.0
19	59965.0
20	63611.0
21	67257.0
22	52696.5
23	38136.0
24	40497.0
25	42858.0
26	42858.0
27	45021.5
28	47185.0
29	48395.5
30	49606.0
31	53213.0
32	56820.0
33	56820.0
34	61736.5
35	66653.0
36	72468.0
37	78283.0
38	81382.5
39	84482.0
40	84482.0
41	87311.0
42	90140.0
43	93580.0
44	97020.0
45	108300.0
46	119580.0
47	119580.0
48	175771.5
49	231963.0
50	210050.5
51	188138.0
52	165168.5
53	142199.0
54	142199.0
55	119150.5
56	96102.0
57	76907.5
58	57713.0
59	50271.5
60	42830.0
61	42830.0
62	37717.5
63	32605.0
64	28175.0
65	23745.0
66	20282.0
67	16819.0
68	16819.0
69	14181.0
70	11543.0
71	9736.0
72	7929.0
73	6493.5
74	5058.0
75	5058.0
76	3939.0
77	2820.0
78	2165.0
79	1510.0
80	1137.0
81	764.0
82	764.0
83	584.0
84	404.0
85	299.0
86	194.0
87	151.0
88	108.0
89	108.0
90	76.5
91	45.0
92	25.5
93	6.0
94	5.5
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1872482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.34064692112265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05176777642126	32.62421694069788
2	12.411773848517308	10.510450683697899
3	4.198957828658941	5.3335977257979605
4	1.9061789881375517	3.228354060207797
5	1.0110374528389985	2.1403989907343615
6	0.6383698677524944	1.6217395905355272
7	0.43466469924198037	1.288278919177646
8	0.31369503989811165	1.0625640740186741
9	0.24799125381394574	0.945009910553651
>10	1.5278537403154233	12.513865526785557
>50	0.15298626788236813	4.45159394630928
>100	0.08874875542298215	7.0579961710554775
>500	0.0062122982050561585	1.7481476975681784
>1k	0.008494366933444134	7.285147596504568
>5k	7.606895761293255E-4	2.7598574456510705
>10k+	5.071263840862169E-4	5.42878072070457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32683	1.7454373393175473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31592	1.6871724267576402	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25656	1.3701600335810973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11201	0.598189995951897	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9534	0.5091637730028914	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8938	0.47733436155861575	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8640	0.461419655836478	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8612	0.45992431435923015	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8256	0.440912115577079	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7433	0.3969597571565441	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4643	0.2479596599593481	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4414	0.23572990287757103	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4260	0.22750552475270788	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4135	0.22082989315785145	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3954	0.21116357860849932	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3845	0.20534242785778448	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3767	0.20117683374259407	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3345	0.17863990147835865	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3091	0.16507501807761035	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2988	0.15957429764344866	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2954	0.1577585258496477	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2851	0.15225780541548598	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2797	0.14937393256650797	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2768	0.14782518603650127	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2750	0.14686389508684194	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2412	0.12881298725435011	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2399	0.12811872156848506	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2337	0.12480760829743623	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2171	0.11594236953946686	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2167	0.11572874932843145	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2134	0.11396638258738935	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2119	0.11316530679600657	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2105	0.11241763605738266	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2059	0.10996100363047548	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2017	0.10771799141460373	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1986	0.10606243477907931	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1923	0.10269791645527167	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1923	0.10269791645527167	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1899	0.10141619518905924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3405052758851623E-5	0.0	0.0	0.0	0.0
2	5.3405052758851623E-5	0.0	0.0	0.0	0.0
3	5.3405052758851623E-5	0.0	0.0	0.0	0.0
4	5.3405052758851623E-5	0.0	0.0	0.0	0.0
5	5.3405052758851623E-5	0.0	0.0	0.0	0.0
6	1.0681010551770325E-4	0.0	0.0	0.0	0.0
7	1.0681010551770325E-4	0.0	0.0	0.0	0.0
8	2.136202110354065E-4	0.0	0.0	0.0	0.0
9	2.136202110354065E-4	0.0	0.0	5.3405052758851623E-5	0.0
10	2.136202110354065E-4	0.0	0.0	1.0681010551770325E-4	0.0
11	2.136202110354065E-4	0.0	0.0	1.6021515827655485E-4	0.0
12	2.136202110354065E-4	0.0	0.0	3.738353693119613E-4	0.0
13	2.136202110354065E-4	0.0	0.0	4.806454748296646E-4	0.0
14	2.136202110354065E-4	0.0	0.0	4.806454748296646E-4	0.0
15	2.136202110354065E-4	0.0	0.0	8.010757913827744E-4	0.0
16	2.136202110354065E-4	0.0	0.0	0.0013351263189712904	0.0
17	2.136202110354065E-4	0.0	0.0	0.0017623667410421036	0.0
18	2.136202110354065E-4	0.0	0.0	0.0020293920048363614	0.0
19	2.136202110354065E-4	0.0	0.0	0.003364518323807652	0.0
20	2.136202110354065E-4	0.0	0.0	0.005554125486920568	0.0
21	2.136202110354065E-4	0.0	0.0	0.01057420044625262	0.0
22	2.136202110354065E-4	0.0	0.0	0.018478148254562662	0.0
23	2.136202110354065E-4	0.0	0.0	0.023925463635965524	0.0
24	2.136202110354065E-4	0.0	0.0	0.031135145758410494	0.0
25	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.03551436008463633	0.0
26	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.044646624106399954	0.0
27	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.10579540951528506	0.0
28	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.1826986854880314	0.0
29	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.27380770549463224	0.0
30	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.4367465214618885	0.0
31	2.136202110354065E-4	0.0	5.3405052758851623E-5	0.6209939534799267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7885	0.0	30.289791	1
ACGTTTA	140	0.0	22.464285	26
GTATTGG	630	0.0	22.31746	1
TACGGAT	60	3.7278987E-5	21.583334	7
CGAATTA	85	1.2461642E-6	19.588236	15
TCACGTT	165	0.0	19.060606	24
ATTGGAC	735	0.0	18.62585	3
GTTATAC	70	1.2197173E-4	18.5	3
GTATCAA	13205	0.0	18.128738	2
CGTTTAT	185	0.0	18.0	27
GATACCT	725	0.0	17.86207	36
GGACCCT	1245	0.0	17.831326	6
TTGGACC	1265	0.0	17.695652	4
TGGACCC	1320	0.0	17.51894	5
GGTATCG	65	0.0015804359	17.076923	35
TAACACT	200	0.0	16.650002	4
TAAGCCG	205	0.0	16.243902	36
CGGTTTA	80	3.3839874E-4	16.1875	15
TAGTGCC	70	0.0025933105	15.857142	7
GACCCTC	1400	0.0	15.592857	7
>>END_MODULE