FastQCFastQC Report
Wed 25 May 2016
SRR2088290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911866
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT387892.0288555787905636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT371581.9435462527185483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT341771.7876252833619093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.6765118475876447No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127680.6678292307096836No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA123130.644030491676718No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG120640.6310065663597763No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG112400.5879073114956801No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102690.5371192332517029No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78530.41075054423270246No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA60370.315764807784646No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC57550.3010148200763024No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53210.27831448438331974No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA51690.2703641363986807No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA50960.26654587716921585No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.24661770228666655No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45720.23913809859059162No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41280.2159147136880932No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40810.21345638240336928No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40400.2113118806443548No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38380.20074628661213706No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32990.1725539342192392No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG31050.16240677955463406No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT30560.15984383842800698No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.1541949069652371No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29090.15215501504812576No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC28750.15037664773577228No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT28630.1497489886843534No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA28240.14770909676724206No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG28100.14697682787392002No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.1424262997511332No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA27230.1424262997511332No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA27050.14148481117400485No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT26450.1383465159169105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC26350.13782346670739476No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT24970.13060538761607768No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA23890.12495645615330782No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23290.12181816089621345No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT22930.11993518374195682No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG22450.11742454753628132No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA22130.11575079006583097No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA20820.10889884542117491No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC20640.1079573568440466No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG20620.10785274700214345No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA20360.10649281905740257No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA20280.10607437968978997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT19700.10304069427459873No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC19610.1025699499860346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85950.031.4682941
GTATTGG5400.025.6944451
ATTGGAC5750.025.4173933
TTAGACG759.271043E-619.7333344
TTGGACC11100.019.1666664
TATTGGA7250.019.1379322
AGACGTA500.00703654818.55
CCGTACG500.00703654818.52
GGACCCT11250.017.5955566
TGGACCC11600.017.5431045
GTATCAA157450.017.23692
GATACTG8500.016.3235336
GTAAGAC1151.2431738E-616.0869563
TGATACT8700.015.948276535
GTCTACG700.002593336415.8571431
GACCCTC12200.015.6188537
TTTTTAC19350.015.4883721
CTTAGAC1201.9370382E-615.4166663
TACGTGA1455.351103E-815.31034536
ATAACGC855.366462E-415.2352943
Produced by FastQC (version 0.11.5)