##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1911866
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.866301822408055	31.0	31.0	33.0	28.0	34.0
2	31.117811080902115	31.0	31.0	34.0	28.0	34.0
3	31.14392797403165	31.0	31.0	34.0	28.0	34.0
4	35.027735730432994	35.0	35.0	37.0	32.0	37.0
5	34.81986708273488	35.0	35.0	37.0	32.0	37.0
6	34.91117421409241	35.0	35.0	37.0	32.0	37.0
7	34.688070712068736	35.0	35.0	37.0	31.0	37.0
8	34.79458654529135	35.0	35.0	37.0	32.0	37.0
9	36.19917190849149	38.0	35.0	39.0	32.0	39.0
10	36.035820502064475	38.0	35.0	39.0	30.0	39.0
11	36.133737929331865	38.0	35.0	39.0	31.0	39.0
12	35.892762881917456	37.0	35.0	39.0	30.0	39.0
13	35.97958591240181	37.0	35.0	39.0	30.0	39.0
14	36.723875522656925	38.0	36.0	40.0	31.0	41.0
15	36.822675856989974	38.0	36.0	40.0	31.0	41.0
16	36.76618497321465	38.0	36.0	40.0	31.0	41.0
17	36.935489725744375	38.0	36.0	40.0	31.0	41.0
18	36.962474357512505	38.0	36.0	40.0	31.0	41.0
19	37.08359320161559	39.0	36.0	40.0	31.0	41.0
20	37.06976744186046	39.0	36.0	40.0	31.0	41.0
21	37.002620999588885	39.0	36.0	40.0	31.0	41.0
22	36.92314262610455	39.0	36.0	40.0	31.0	41.0
23	36.79381557075653	39.0	35.0	40.0	30.0	41.0
24	36.70526020129026	38.0	35.0	40.0	30.0	41.0
25	36.56184638463156	38.0	35.0	40.0	30.0	41.0
26	36.28822155946076	38.0	35.0	40.0	30.0	41.0
27	36.13859234904538	38.0	35.0	40.0	30.0	41.0
28	35.94472729783364	38.0	34.0	40.0	29.0	41.0
29	35.72193344094199	38.0	34.0	40.0	29.0	41.0
30	35.442429019607026	38.0	34.0	40.0	27.0	41.0
31	35.10472962017212	37.0	34.0	40.0	27.0	41.0
32	34.96916467995142	37.0	33.0	40.0	27.0	41.0
33	34.7379790215423	37.0	33.0	40.0	26.0	41.0
34	34.80777261586324	37.0	33.0	40.0	25.0	41.0
35	34.62969266674547	37.0	33.0	40.0	25.0	41.0
36	34.494116742491364	37.0	33.0	40.0	24.0	41.0
37	34.32515720243992	37.0	33.0	40.0	24.0	41.0
38	34.18507416314742	37.0	33.0	40.0	23.0	41.0
39	34.001528349790206	37.0	33.0	40.0	21.0	41.0
40	33.719559843629206	37.0	33.0	40.0	20.0	41.0
41	33.5208215429324	37.0	33.0	40.0	18.0	41.0
42	33.271372575274626	37.0	32.0	40.0	17.0	41.0
43	32.86894740530979	37.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	13.0
16	46.0
17	90.0
18	224.0
19	529.0
20	1089.0
21	2100.0
22	3806.0
23	6605.0
24	10343.0
25	16234.0
26	23946.0
27	34542.0
28	45979.0
29	60231.0
30	75119.0
31	88573.0
32	104852.0
33	123813.0
34	144977.0
35	169891.0
36	206659.0
37	262879.0
38	289757.0
39	239565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13949408588259	22.544937772835542	15.465205197435386	20.85036294384648
2	18.787299946753592	24.65669665133435	35.86893642127639	20.68706698063567
3	20.35676140482649	25.362865389101536	34.55351996426528	19.726853241806698
4	12.848337697307239	16.46632138444849	36.10373321142799	34.58160770681628
5	10.934448334768232	39.82564677650003	35.10350620807107	14.136398680660674
6	30.777680025692177	37.04171735885255	12.4380578973631	19.742544718092166
7	23.215696079118516	36.007282937193295	21.17381657501101	19.60320440867718
8	26.949273641562744	34.03292908603427	18.832386788613846	20.185410483789134
9	23.24938044821133	14.323336468141596	24.143428462036564	38.28385462161051
10	15.93061438406248	27.858908521831548	33.75388233275763	22.456594761348335
11	30.695665909640113	22.08920499658449	25.862272774347154	21.352856319428245
12	21.50998030196677	27.81695997522839	32.10047147655746	18.572588246247385
13	32.903822757452666	20.954815870986774	28.909139029618185	17.232222341942375
14	19.511566187170022	24.035000360903954	30.430166130889923	26.023267321036098
15	20.683144111564307	29.369213114308216	29.549978921116853	20.39766385301062
16	19.35423298494769	29.35885673995981	29.847280091805594	21.439630183286905
17	20.196394517188967	26.141319527623796	28.530137572403085	25.13214838278415
18	22.750077672807613	23.61483493089997	33.46678062165444	20.16830677463797
19	21.63362913509629	22.851863048979375	35.56389412228682	19.950613693637525
20	23.11840892614859	21.43152292053941	36.11199738893834	19.338070764373654
21	21.240505349224264	23.081690871640586	37.448701948776744	18.229101830358406
22	18.400452751395758	23.31031568111991	36.7274693937755	21.561762173708825
23	20.604372900611235	23.818562598006345	38.38286783697184	17.19419666441058
24	20.403731223841	23.458077082808103	34.47725938951788	21.660932303833007
25	20.093249213072465	24.912467714787542	35.59187725499591	19.40240581714409
26	19.642851538758467	25.85233483936636	35.42063094380046	19.08418267807472
27	17.533550991544384	26.24252954966509	35.08729168257608	21.136627776214443
28	18.745612924755186	25.002902923112813	35.03775892243494	21.21372522969706
29	18.96780422895747	24.77281357584684	33.64775564814689	22.6116265470488
30	19.66717332700095	25.056515467088175	36.71287632083002	18.56343488508086
31	21.509091118310593	24.83945004513915	35.53789857657388	18.11356025997638
32	20.810558899002334	24.88913972004314	33.899237708082055	20.40106367287247
33	19.752901092440577	24.93799251621191	35.057896317001294	20.251210074346215
34	18.0759530218122	26.21098968233129	36.244851888155345	19.468205407701166
35	17.38432505206955	29.659610035431356	33.82187872999467	19.134186182504422
36	21.615479327526092	25.879690313024028	33.1610583586925	19.343772000757376
37	20.498507740605252	27.61647521322101	32.685606627242706	19.199410418931034
38	20.213602836182034	24.901902120755327	34.42448372427775	20.460011318784893
39	19.30674011672366	24.67055745538652	35.344736503499725	20.677965924390097
40	17.27757070840739	25.71440676281706	34.34294035251425	22.665082176261304
41	20.51566375467737	24.617258740936865	34.110026539516895	20.757050964868874
42	18.427651310290575	28.16954744736294	32.79382550869151	20.608975733654976
43	19.405596417322137	28.57349835187194	30.703773172387606	21.31713205841832
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	164.5
2	226.0
3	749.0
4	1272.0
5	1272.0
6	2546.5
7	3821.0
8	4410.0
9	4999.0
10	7888.5
11	10778.0
12	10778.0
13	21249.0
14	31720.0
15	54130.5
16	76541.0
17	73760.0
18	70979.0
19	70979.0
20	73123.0
21	75267.0
22	57809.0
23	40351.0
24	41824.5
25	43298.0
26	43298.0
27	44908.5
28	46519.0
29	46545.0
30	46571.0
31	49728.0
32	52885.0
33	52885.0
34	57362.5
35	61840.0
36	67344.0
37	72848.0
38	76030.0
39	79212.0
40	79212.0
41	81643.0
42	84074.0
43	88482.0
44	92890.0
45	108798.0
46	124706.0
47	124706.0
48	197211.5
49	269717.0
50	238873.0
51	208029.0
52	177098.5
53	146168.0
54	146168.0
55	119892.0
56	93616.0
57	72933.0
58	52250.0
59	44755.5
60	37261.0
61	37261.0
62	32286.5
63	27312.0
64	23335.5
65	19359.0
66	16468.5
67	13578.0
68	13578.0
69	11261.5
70	8945.0
71	7561.0
72	6177.0
73	4996.0
74	3815.0
75	3815.0
76	3041.0
77	2267.0
78	1721.5
79	1176.0
80	920.5
81	665.0
82	665.0
83	492.5
84	320.0
85	243.0
86	166.0
87	123.5
88	81.0
89	81.0
90	65.5
91	50.0
92	30.5
93	11.0
94	7.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1911866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.19019403747282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75893193209994	28.93248310558592
2	12.724803511085547	9.71925430354145
3	4.489868199041544	5.144068131722256
4	2.084052074994662	3.18361412512976
5	1.11598640119725	2.1309868602451982
6	0.6967100999770381	1.5964496343594106
7	0.4821117395208121	1.2888358616030358
8	0.3549421807202462	1.0844248603031958
9	0.26830401736119214	0.9221924235651661
>10	1.7320451758178523	12.58220926891288
>50	0.17036361453655727	4.481041678369111
>100	0.09979629681288625	7.102061599942076
>500	0.01035222976616096	2.7058004729268537
>1k	0.009662081115083564	7.690683031320149
>5k	8.281783812928769E-4	1.857160655789713
>10k+	0.0012422675719393154	9.578733986683897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38789	2.0288555787905636	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37158	1.9435462527185483	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34177	1.7876252833619093	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12934	0.6765118475876447	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12768	0.6678292307096836	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12313	0.644030491676718	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12064	0.6310065663597763	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11240	0.5879073114956801	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10269	0.5371192332517029	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7853	0.41075054423270246	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6037	0.315764807784646	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5755	0.3010148200763024	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5321	0.27831448438331974	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5169	0.2703641363986807	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5096	0.26654587716921585	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4715	0.24661770228666655	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4572	0.23913809859059162	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4128	0.2159147136880932	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4081	0.21345638240336928	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4040	0.2113118806443548	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3838	0.20074628661213706	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3299	0.1725539342192392	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3105	0.16240677955463406	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3056	0.15984383842800698	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2948	0.1541949069652371	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2909	0.15215501504812576	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2875	0.15037664773577228	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2863	0.1497489886843534	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2824	0.14770909676724206	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	2810	0.14697682787392002	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2723	0.1424262997511332	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2723	0.1424262997511332	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2705	0.14148481117400485	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2645	0.1383465159169105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2635	0.13782346670739476	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2497	0.13060538761607768	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2389	0.12495645615330782	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2329	0.12181816089621345	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	2293	0.11993518374195682	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	2245	0.11742454753628132	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2213	0.11575079006583097	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	2082	0.10889884542117491	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2064	0.1079573568440466	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2062	0.10785274700214345	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA	2036	0.10649281905740257	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2028	0.10607437968978997	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1970	0.10304069427459873	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1961	0.1025699499860346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.2304920951572963E-5	0.0
3	0.0	0.0	0.0	5.2304920951572963E-5	0.0
4	0.0	0.0	0.0	1.0460984190314593E-4	0.0
5	0.0	0.0	0.0	1.569147628547189E-4	0.0
6	2.0921968380629185E-4	0.0	0.0	1.569147628547189E-4	0.0
7	2.0921968380629185E-4	0.0	0.0	1.569147628547189E-4	0.0
8	2.0921968380629185E-4	0.0	0.0	2.0921968380629185E-4	0.0
9	2.0921968380629185E-4	0.0	0.0	2.6152460475786484E-4	0.0
10	2.0921968380629185E-4	0.0	0.0	5.753541304673026E-4	0.0
11	2.0921968380629185E-4	0.0	0.0	9.937934980798863E-4	0.0
12	2.0921968380629185E-4	0.0	0.0	0.0017260623914019078	0.0
13	2.0921968380629185E-4	0.0	0.0	0.0018306722333050539	0.0
14	2.0921968380629185E-4	0.0	0.0	0.0020398919171113455	0.0
15	2.6152460475786484E-4	0.0	0.0	0.0027198558894817944	0.0
16	2.6152460475786484E-4	0.0	0.0	0.0036613444666101077	0.0
17	2.6152460475786484E-4	0.0	0.0	0.005387406858012016	0.0
18	2.6152460475786484E-4	0.0	0.0	0.006904249565607631	0.0
19	2.6152460475786484E-4	0.0	0.0	0.008734921798912686	0.0
20	2.6152460475786484E-4	0.0	0.0	0.014226938498827847	0.0
21	2.6152460475786484E-4	0.0	0.0	0.0282446573138494	0.0
22	2.6152460475786484E-4	0.0	0.0	0.04592372059548106	0.0
23	2.6152460475786484E-4	0.0	0.0	0.05659392446960195	0.0
24	2.6152460475786484E-4	0.0	0.0	0.07176235154555811	0.0
25	3.138295257094378E-4	0.0	0.0	0.07929426016258462	0.0
26	3.138295257094378E-4	0.0	0.0	0.0907490378509791	5.2304920951572963E-5
27	3.138295257094378E-4	0.0	0.0	0.16449897639269698	5.2304920951572963E-5
28	3.138295257094378E-4	0.0	0.0	0.26654587716921585	5.2304920951572963E-5
29	3.138295257094378E-4	0.0	0.0	0.3802044703969839	5.2304920951572963E-5
30	3.138295257094378E-4	0.0	0.0	0.5967991480574475	5.2304920951572963E-5
31	3.138295257094378E-4	0.0	0.0	0.846973584968821	5.2304920951572963E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8595	0.0	31.468294	1
GTATTGG	540	0.0	25.694445	1
ATTGGAC	575	0.0	25.417393	3
TTAGACG	75	9.271043E-6	19.733334	4
TTGGACC	1110	0.0	19.166666	4
TATTGGA	725	0.0	19.137932	2
AGACGTA	50	0.007036548	18.5	5
CCGTACG	50	0.007036548	18.5	2
GGACCCT	1125	0.0	17.595556	6
TGGACCC	1160	0.0	17.543104	5
GTATCAA	15745	0.0	17.2369	2
GATACTG	850	0.0	16.32353	36
GTAAGAC	115	1.2431738E-6	16.086956	3
TGATACT	870	0.0	15.9482765	35
GTCTACG	70	0.0025933364	15.857143	1
GACCCTC	1220	0.0	15.618853	7
TTTTTAC	1935	0.0	15.488372	1
CTTAGAC	120	1.9370382E-6	15.416666	3
TACGTGA	145	5.351103E-8	15.310345	36
ATAACGC	85	5.366462E-4	15.235294	3
>>END_MODULE