FastQCFastQC Report
Wed 25 May 2016
SRR2088289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732615
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161992.2111204384294614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142121.9399002204432068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91131.2439002750421435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.746367464493629No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA35860.4894794673873726No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG30400.41495191881138116No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26170.3572135432662449No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21250.29005685114282403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.2619383987496844No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16920.23095350218054503No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.21662128130054667No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15790.2155293025668325No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13460.18372542194740757No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT13100.17881151764569386No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12490.17048517980112338No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11470.15656245094626783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11470.15656245094626783No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT10790.14728063170969743No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.14714413436798318No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC9700.132402421462842No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA9690.13226592412112775No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG9180.12530455969369997No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA8620.11766070855770085No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8610.11752421121598658No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA8560.11684172450741521No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.11424827501484408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC8290.11315629628112993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7840.10701391590398777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7630.1041474717279881No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7420.10128102755198844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.030.4770361
GATAATC250.005495058429.67
CCGTGGA652.680641E-622.769232
ACGTTTA806.9530324E-720.812526
CGTGGAT555.140648E-420.1818183
TATACTG759.25825E-619.7333345
GTATCAA52550.019.6089442
TACCCCA500.00703250618.55
CGCGCCC500.00703250618.526
TAGTTCG701.21843186E-418.59
AAAACGG902.150049E-618.49999826
TCACGTT902.150049E-618.49999824
GTATTGG1800.017.4722211
TTAAGGT650.001579194717.0769234
GACCCTC2700.016.4444457
GTGGTAT4950.016.4444431
TCGAACC904.4424185E-516.44444331
GATTAGA803.3804716E-416.18752
TATCCTG1151.2406144E-616.0869565
GGACCCT2650.016.0566046
Produced by FastQC (version 0.11.5)