##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732615
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.154423537601605	31.0	30.0	31.0	27.0	34.0
2	30.370651706558014	31.0	30.0	31.0	27.0	34.0
3	30.248482490803493	31.0	30.0	31.0	26.0	34.0
4	34.108433488257816	35.0	33.0	37.0	30.0	37.0
5	33.75715348443589	35.0	33.0	37.0	28.0	37.0
6	34.06764944752701	35.0	33.0	37.0	30.0	37.0
7	33.8647188496004	35.0	33.0	37.0	29.0	37.0
8	34.05594753042185	35.0	33.0	37.0	30.0	37.0
9	35.349165659998775	37.0	34.0	39.0	30.0	39.0
10	35.04536762146557	37.0	34.0	39.0	29.0	39.0
11	35.295518109784815	37.0	34.0	39.0	30.0	39.0
12	34.96805689209203	37.0	34.0	39.0	27.0	39.0
13	35.14432000436791	37.0	34.0	39.0	29.0	39.0
14	35.74018140496714	38.0	34.0	40.0	27.0	41.0
15	35.888321969929635	38.0	34.0	40.0	29.0	41.0
16	35.7080417408871	38.0	34.0	40.0	27.0	41.0
17	35.9002545675423	38.0	34.0	40.0	29.0	41.0
18	35.95677402182592	38.0	34.0	40.0	29.0	41.0
19	36.0783740436655	38.0	34.0	40.0	29.0	41.0
20	36.040861844215584	38.0	34.0	40.0	29.0	41.0
21	35.96014414119285	38.0	34.0	40.0	29.0	41.0
22	35.85611951707241	38.0	34.0	40.0	28.0	41.0
23	35.68097158807832	38.0	34.0	40.0	27.0	41.0
24	35.59916327129529	38.0	34.0	40.0	27.0	41.0
25	35.452080560731076	38.0	34.0	40.0	27.0	41.0
26	35.083753403902456	37.0	33.0	40.0	27.0	41.0
27	34.85879349999659	37.0	33.0	40.0	26.0	41.0
28	34.64594500522102	37.0	33.0	40.0	25.0	41.0
29	34.43483275663206	37.0	33.0	39.0	25.0	40.0
30	34.16256560403486	36.0	32.0	39.0	25.0	40.0
31	33.82302027668011	36.0	32.0	39.0	24.0	40.0
32	33.64093418780669	36.0	32.0	39.0	24.0	40.0
33	33.40849422957488	35.0	31.0	39.0	23.0	40.0
34	33.465868157217635	35.0	31.0	39.0	23.0	40.0
35	33.31283143260785	35.0	31.0	39.0	22.0	40.0
36	33.17627266708981	35.0	31.0	39.0	21.0	40.0
37	33.007104686636225	35.0	31.0	39.0	21.0	40.0
38	32.861594425448565	35.0	31.0	39.0	19.0	40.0
39	32.61664994574231	35.0	31.0	39.0	18.0	40.0
40	32.35473065662046	35.0	30.0	39.0	15.0	40.0
41	32.136740306982524	35.0	30.0	39.0	15.0	40.0
42	31.898118384144468	35.0	30.0	39.0	13.0	40.0
43	31.448992990861502	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	7.0
15	12.0
16	41.0
17	105.0
18	250.0
19	568.0
20	1034.0
21	1820.0
22	3000.0
23	4762.0
24	7057.0
25	10204.0
26	14085.0
27	18686.0
28	24392.0
29	30639.0
30	36763.0
31	42696.0
32	48938.0
33	56659.0
34	64862.0
35	74658.0
36	87591.0
37	95439.0
38	78416.0
39	29926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69369996519318	26.132416071197014	15.342437706025674	18.831446257584133
2	23.890310736198412	26.70898084259809	29.935095514014865	19.465612907188632
3	25.42959125871024	25.439009575288523	31.650321109996383	17.481078056004858
4	19.521303822608054	19.43926892023778	31.836503484094646	29.20292377305952
5	11.108699658074158	42.58512315472656	33.53234645755274	12.77383072964654
6	31.368727094039844	37.858493205844816	12.77574169243054	17.9970380076848
7	22.626208854582558	36.41148488633184	22.226544638043176	18.73576162104243
8	27.092674870156902	36.42595360455355	18.8751254069327	17.606246118356843
9	23.846631586849846	14.069463497198392	23.634924209851015	38.44898070610075
10	16.97439992356149	29.8531971089863	32.82815667164882	20.34424629580339
11	32.030193211987196	22.57829828764085	23.85659589279499	21.534912607576967
12	18.914300144004695	29.588528763402334	32.87279130238939	18.624379790203584
13	32.195764487486606	21.26137193478157	28.832742982330416	17.710120595401406
14	18.94064413095555	24.146516246596097	31.20274632651529	25.710093295933063
15	21.894856097677497	30.159633641134835	28.809538434238995	19.13597182694867
16	19.35136463217379	28.701432539601292	31.050551790503878	20.89665103772104
17	19.540413450448053	27.819796209468823	30.793117804030768	21.84667253605236
18	20.695317458692493	24.789691720753737	33.6085119742293	20.906478846324468
19	20.811203701807905	24.106795520157245	36.25587791677757	18.82612286125728
20	21.181247995195292	23.78534428042014	36.569958300062105	18.463449424322462
21	20.329641080239963	24.372965336500073	37.23824928509517	18.059144298164792
22	18.441882844331605	24.66315868498461	36.79640738996608	20.0985510807177
23	19.715403042525747	24.892747213748013	37.994717552875656	17.397132190850584
24	19.49850876654177	25.007814472813145	35.55564655378337	19.938030206861722
25	19.501238713376058	25.828845983224475	35.98615916955018	18.683756133849293
26	19.182653917814953	26.554192857094105	35.65747357070221	18.605679654388734
27	18.527466677586453	26.27805873480614	35.47838905837309	19.716085529234316
28	18.641578455259584	26.627901421619814	35.860854609856474	18.86966551326413
29	19.028684916361254	25.14390232250227	34.968707984412006	20.858704776724473
30	19.33198200965036	26.032636514403883	36.40356804051241	18.231813435433345
31	19.984166308361146	25.92234666229875	35.72299229472506	18.370494734615043
32	19.787200644267454	25.744217631361625	35.18041536141084	19.288166362960084
33	19.228926516656088	26.163400967766155	35.31472874565768	19.292943769920083
34	18.517229376957882	26.37565433413184	36.10846078772616	18.998655501184114
35	17.992124103383087	28.05620960531794	34.96502255618572	18.986643735113258
36	20.789364127133624	25.762781269834772	33.985517632044115	19.46233697098749
37	19.759491683899455	27.044354811190054	33.703787118745865	19.49236638616463
38	19.950724459641147	25.19440633893655	34.80627614777202	20.04859305365028
39	18.867891047821843	25.379360236959386	35.4000395842291	20.352709130989673
40	18.02870539096251	25.732069367949062	34.23721873016523	22.0020065109232
41	19.645516403568042	24.887696812104583	34.37876647352292	21.08802031080445
42	18.959071272086973	26.953584078950062	32.83334357063396	21.254001078328997
43	19.51570743159777	26.918231267446068	31.679258546439808	21.886802754516353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	105.0
2	124.0
3	266.0
4	408.0
5	408.0
6	865.5
7	1323.0
8	1542.0
9	1761.0
10	2868.5
11	3976.0
12	3976.0
13	8612.5
14	13249.0
15	21943.5
16	30638.0
17	27782.0
18	24926.0
19	24926.0
20	28776.5
21	32627.0
22	25347.5
23	18068.0
24	18273.5
25	18479.0
26	18479.0
27	19229.5
28	19980.0
29	20865.5
30	21751.0
31	22877.5
32	24004.0
33	24004.0
34	26297.5
35	28591.0
36	30491.0
37	32391.0
38	34054.0
39	35717.0
40	35717.0
41	37123.5
42	38530.0
43	39800.5
44	41071.0
45	44288.5
46	47506.0
47	47506.0
48	63451.0
49	79396.0
50	71142.5
51	62889.0
52	54150.5
53	45412.0
54	45412.0
55	38705.5
56	31999.0
57	27045.0
58	22091.0
59	19407.0
60	16723.0
61	16723.0
62	14690.5
63	12658.0
64	10874.0
65	9090.0
66	7619.0
67	6148.0
68	6148.0
69	5197.5
70	4247.0
71	3486.5
72	2726.0
73	2258.0
74	1790.0
75	1790.0
76	1406.5
77	1023.0
78	812.0
79	601.0
80	451.0
81	301.0
82	301.0
83	234.0
84	167.0
85	120.5
86	74.0
87	59.5
88	45.0
89	45.0
90	34.0
91	23.0
92	13.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	732615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.44124011935159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.42838832396585	47.36255790078202
2	8.44119684060779	9.359808418696968
3	2.352916782340051	3.913458729317007
4	1.0098200367016652	2.2394270052843788
5	0.5741116433141488	1.5914730736147626
6	0.37265747909335023	1.2396355668412007
7	0.27183832125988294	1.0549737549827425
8	0.21383419634626494	0.9484186420289479
9	0.15861327970209155	0.7914345233473379
>10	1.0313248873388758	10.841141125127013
>50	0.08653977279297394	3.2793172923544405
>100	0.04720343018888101	4.966061808484241
>500	0.006883833569163677	2.6675851032955813
>1k	0.0036877679834805416	3.612164571173481
>5k	4.917023977974055E-4	1.9874333652420861
>10k+	4.917023977974055E-4	4.145109119427823
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16199	2.2111204384294614	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14212	1.9399002204432068	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9113	1.2439002750421435	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5468	0.746367464493629	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3586	0.4894794673873726	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3040	0.41495191881138116	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2617	0.3572135432662449	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2125	0.29005685114282403	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1919	0.2619383987496844	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1692	0.23095350218054503	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1587	0.21662128130054667	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1579	0.2155293025668325	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1346	0.18372542194740757	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1310	0.17881151764569386	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1249	0.17048517980112338	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1147	0.15656245094626783	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1147	0.15656245094626783	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1079	0.14728063170969743	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1078	0.14714413436798318	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	970	0.132402421462842	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	969	0.13226592412112775	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	918	0.12530455969369997	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	862	0.11766070855770085	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	861	0.11752421121598658	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	856	0.11684172450741521	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.11424827501484408	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	829	0.11315629628112993	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	784	0.10701391590398777	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	763	0.1041474717279881	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	742	0.10128102755198844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.3649734171427013E-4	0.0	0.0	0.0	0.0
7	1.3649734171427013E-4	0.0	0.0	0.0	0.0
8	4.094920251428103E-4	0.0	0.0	0.0	0.0
9	4.094920251428103E-4	0.0	0.0	2.7299468342854025E-4	1.3649734171427013E-4
10	4.094920251428103E-4	0.0	0.0	2.7299468342854025E-4	1.3649734171427013E-4
11	4.094920251428103E-4	0.0	0.0	2.7299468342854025E-4	1.3649734171427013E-4
12	4.094920251428103E-4	0.0	0.0	4.094920251428103E-4	1.3649734171427013E-4
13	5.459893668570805E-4	0.0	0.0	6.824867085713506E-4	1.3649734171427013E-4
14	5.459893668570805E-4	0.0	0.0	6.824867085713506E-4	2.7299468342854025E-4
15	5.459893668570805E-4	0.0	0.0	9.554813919998908E-4	2.7299468342854025E-4
16	5.459893668570805E-4	0.0	0.0	0.0015014707588569712	2.7299468342854025E-4
17	5.459893668570805E-4	0.0	0.0	0.0020474601257140517	2.7299468342854025E-4
18	5.459893668570805E-4	0.0	0.0	0.002320454809142592	2.7299468342854025E-4
19	5.459893668570805E-4	0.0	0.0	0.0028664441759996724	2.7299468342854025E-4
20	5.459893668570805E-4	0.0	0.0	0.005596391010285075	2.7299468342854025E-4
21	5.459893668570805E-4	0.0	0.0	0.01078328999542734	2.7299468342854025E-4
22	5.459893668570805E-4	0.0	0.0	0.02033810391542625	2.7299468342854025E-4
23	5.459893668570805E-4	0.0	0.0	0.02497901353371143	2.7299468342854025E-4
24	5.459893668570805E-4	0.0	0.0	0.030848399227425046	2.7299468342854025E-4
25	5.459893668570805E-4	0.0	0.0	0.03603529821256731	2.7299468342854025E-4
26	5.459893668570805E-4	0.0	0.0	0.043269657323423624	2.7299468342854025E-4
27	5.459893668570805E-4	0.0	0.0	0.10933437071313036	2.7299468342854025E-4
28	5.459893668570805E-4	0.0	0.0	0.18099547511312217	2.7299468342854025E-4
29	5.459893668570805E-4	0.0	0.0	0.2579799758399705	2.7299468342854025E-4
30	6.824867085713506E-4	0.0	0.0	0.40266715805709685	2.7299468342854025E-4
31	8.189840502856206E-4	0.0	0.0	0.5828436491199334	2.7299468342854025E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3375	0.0	30.477036	1
GATAATC	25	0.0054950584	29.6	7
CCGTGGA	65	2.680641E-6	22.76923	2
ACGTTTA	80	6.9530324E-7	20.8125	26
CGTGGAT	55	5.140648E-4	20.181818	3
TATACTG	75	9.25825E-6	19.733334	5
GTATCAA	5255	0.0	19.608944	2
TACCCCA	50	0.007032506	18.5	5
CGCGCCC	50	0.007032506	18.5	26
TAGTTCG	70	1.21843186E-4	18.5	9
AAAACGG	90	2.150049E-6	18.499998	26
TCACGTT	90	2.150049E-6	18.499998	24
GTATTGG	180	0.0	17.472221	1
TTAAGGT	65	0.0015791947	17.076923	4
GACCCTC	270	0.0	16.444445	7
GTGGTAT	495	0.0	16.444443	1
TCGAACC	90	4.4424185E-5	16.444443	31
GATTAGA	80	3.3804716E-4	16.1875	2
TATCCTG	115	1.2406144E-6	16.086956	5
GGACCCT	265	0.0	16.056604	6
>>END_MODULE