FastQCFastQC Report
Wed 25 May 2016
SRR2088288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524557
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66651.2705959504877449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62631.1939598556496245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54481.0385906584031859No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27450.5232987072901515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.4518098128516062No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT18220.34734070844541204No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16170.3082601128190073No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT15490.2952967932941511No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14440.27527990285135834No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA14270.27203907297014435No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT13750.262125946274666No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT12200.23257720324006737No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG11780.22457044706295026No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11190.21332286100461917No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9810.1870149478512345No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9260.1765299100002478No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.16833251677129465No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7690.14659989286197686No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7380.1406901442550571No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7360.14030887015138488No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.11819497213839487No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA6080.11590732751636142No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC5750.10961630480576944No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT5670.10809120839108048No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG5460.10408783030252193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.028.6347851
ATGGGCG358.8629423E-426.4285719
ATCCACG802.7186616E-823.1251
ATAGTCG400.001929795623.12520
TAGGCGC450.00382307820.55555513
TCTATAC450.00382307820.5555553
GTATTGG1101.7480488E-920.1818181
CGGCTCT851.2426681E-619.5882366
ATTGGAC1052.2524546E-819.380953
CGTCTGT1501.2732926E-1118.534
ACGGCTC902.1476808E-618.55
CCCGCAT609.2276925E-418.513
CACGGCT953.5988305E-617.5263164
TAAGCAA752.0645677E-417.26666610
GTATCAA29850.017.229482
ATAGCCT650.001578385817.0769253
TTGGACC2450.016.6122444
GCGTCTG1708.367351E-1116.3235333
GAAGACT803.3781826E-416.18754
GGACCCT2600.015.6538486
Produced by FastQC (version 0.11.5)