##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524557
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88707995508591	31.0	30.0	33.0	27.0	34.0
2	30.764290629998264	31.0	31.0	34.0	27.0	34.0
3	30.74868317456444	31.0	31.0	34.0	27.0	34.0
4	34.95117213191322	35.0	35.0	37.0	32.0	37.0
5	34.8646629441605	35.0	35.0	37.0	32.0	37.0
6	34.96996513248322	35.0	35.0	37.0	32.0	37.0
7	34.56739496375037	35.0	35.0	37.0	30.0	37.0
8	34.801541109927044	35.0	35.0	37.0	32.0	37.0
9	36.20260143320935	38.0	35.0	39.0	32.0	39.0
10	36.16564072159937	38.0	35.0	39.0	32.0	39.0
11	36.2606237263062	38.0	35.0	39.0	32.0	39.0
12	35.98814809448735	38.0	35.0	39.0	30.0	39.0
13	36.148344221886276	38.0	35.0	39.0	32.0	39.0
14	37.034817951147346	39.0	36.0	40.0	31.0	41.0
15	37.10021980452077	39.0	36.0	40.0	31.0	41.0
16	37.01060132645261	39.0	36.0	40.0	31.0	41.0
17	37.128544657682575	39.0	36.0	40.0	31.0	41.0
18	37.12511319074953	39.0	36.0	40.0	31.0	41.0
19	37.22286424544902	39.0	36.0	40.0	31.0	41.0
20	37.18727993335329	39.0	36.0	40.0	31.0	41.0
21	37.107830035630066	39.0	36.0	40.0	31.0	41.0
22	37.04972004948938	39.0	36.0	40.0	31.0	41.0
23	36.904199162340795	39.0	35.0	40.0	31.0	41.0
24	36.83344803329285	39.0	35.0	40.0	31.0	41.0
25	36.755391692418556	38.0	35.0	40.0	30.0	41.0
26	36.497526484252425	38.0	35.0	40.0	30.0	41.0
27	36.3416597243007	38.0	35.0	40.0	30.0	41.0
28	36.16837255055218	38.0	35.0	40.0	30.0	41.0
29	36.01385168818641	38.0	34.0	40.0	29.0	41.0
30	35.8406064545893	38.0	34.0	40.0	29.0	41.0
31	35.57251928770372	38.0	34.0	40.0	28.0	41.0
32	35.3009091481002	38.0	34.0	40.0	27.0	41.0
33	35.41103064109334	38.0	34.0	40.0	27.0	41.0
34	35.381573403843625	38.0	34.0	40.0	27.0	41.0
35	35.298402270868564	38.0	34.0	40.0	27.0	41.0
36	35.19741610539941	38.0	34.0	40.0	26.0	41.0
37	35.03653177824336	38.0	33.0	40.0	25.0	41.0
38	34.91019660399156	38.0	33.0	40.0	25.0	41.0
39	34.841715962230985	38.0	33.0	40.0	24.0	41.0
40	34.6913357366311	38.0	33.0	40.0	24.0	41.0
41	34.525471969681085	38.0	33.0	40.0	23.0	41.0
42	34.31799975979732	38.0	33.0	40.0	22.0	41.0
43	33.95775101657208	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	20.0
18	42.0
19	112.0
20	215.0
21	442.0
22	809.0
23	1351.0
24	2249.0
25	3587.0
26	5353.0
27	7893.0
28	10816.0
29	14597.0
30	18667.0
31	22624.0
32	27425.0
33	33586.0
34	39998.0
35	47578.0
36	57612.0
37	71810.0
38	81514.0
39	76251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.62611689482745	25.696349491094388	14.000194449792872	21.677339164285293
2	17.68558993588876	23.656342399396063	35.9875094603637	22.670558204351483
3	19.671074830761956	26.704247584151965	32.523062317345875	21.101615267740208
4	13.121357640828357	16.43253259416994	35.7553135312273	34.6907962337744
5	10.754789279334753	40.91681170969027	35.012591577273774	13.315807433701199
6	30.917517066782064	36.73957262985719	15.671700120291979	16.67121018306876
7	23.448166738791016	35.903629157555805	22.114851198249188	18.533352905403987
8	27.215917431280108	34.08838314997226	20.638557868830272	18.057141549917358
9	24.87718207935458	14.037559311952752	24.48637612309053	36.59888248560213
10	16.08843271560574	28.641882579014293	34.090670794594296	21.17901391078567
11	30.628130022094837	22.562848270064073	25.35625299061875	21.45276871722234
12	19.41695564066441	28.38452255903553	32.08879111326319	20.109730687036873
13	30.2020562112411	21.92516733167225	29.27270058354002	18.600075873546633
14	20.227163110967922	23.742129072722314	29.167087656822805	26.863620159486956
15	22.608410525452907	29.848996391240608	27.26491115360199	20.277681929704492
16	20.963594042210858	29.16060599706038	27.93519102785779	21.940608932870976
17	18.69844459229407	28.20208290042836	28.752833343182914	24.346639164094654
18	19.713205619217742	27.35336674565395	33.02062502263815	19.912802612490157
19	18.981731251322547	26.222507754162084	34.560781764422174	20.234979230093202
20	23.202054304870586	24.94257058813437	33.215074815510995	18.640300291484053
21	22.367826566035724	25.961716267250267	31.98851602399739	19.681941142716617
22	21.00610610477031	25.65879399188267	32.50266415279941	20.832435750547603
23	19.881728773040873	27.52055544011423	33.53992035183974	19.057795435005158
24	18.69844459229407	26.912423244757004	32.28934129179479	22.099790871154134
25	19.767155904887364	27.71519587003891	33.265593634247566	19.252054590826166
26	21.148893256595567	26.674317567013688	31.855070087712107	20.321719088678638
27	19.983719595773195	27.91612732267418	31.473986621091704	20.626166460460922
28	19.633519331550243	27.481474844487824	31.724293070152527	21.160712753809406
29	18.72017721620339	27.279780843645206	32.695970123361235	21.304071816790167
30	18.192875130824675	27.52799028513584	34.68831795210053	19.59081663193895
31	19.63370996860208	27.04586918104229	33.388554532681866	19.931866317673773
32	20.44506126121661	27.47041789548133	31.58989394860806	20.494626894693997
33	21.43923348654198	27.30627939385043	31.02351126760295	20.230975852004644
34	19.66745272677707	29.639676908324546	31.060494855659154	19.632375509239225
35	18.446994320922226	30.399937471047	31.295359703521257	19.857708504509517
36	19.580712868191636	27.115451704962474	33.16913128601849	20.134704140827402
37	19.518946463396734	27.28511868109662	32.38199089898715	20.813943956519502
38	19.94216071847292	26.87181755271591	32.95599906206571	20.230022666745466
39	20.64008296524496	26.401706582888036	32.89194501264877	20.06626543921824
40	19.035109625836657	26.937968609703045	32.27752179458095	21.749399969879345
41	20.002973938008644	26.42858640719693	32.013108203684254	21.555331451110174
42	17.822086065003422	27.980753283246624	32.39533549261567	21.801825159134278
43	18.321173866710385	27.64751971663709	32.135497190963044	21.895809225689487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	97.5
2	110.0
3	257.0
4	404.0
5	404.0
6	689.5
7	975.0
8	1130.5
9	1286.0
10	1874.0
11	2462.0
12	2462.0
13	4500.0
14	6538.0
15	10637.0
16	14736.0
17	14444.0
18	14152.0
19	14152.0
20	15237.0
21	16322.0
22	13825.0
23	11328.0
24	12276.0
25	13224.0
26	13224.0
27	14397.0
28	15570.0
29	16289.5
30	17009.0
31	18168.5
32	19328.0
33	19328.0
34	20790.0
35	22252.0
36	23761.0
37	25270.0
38	25983.5
39	26697.0
40	26697.0
41	27467.5
42	28238.0
43	31498.5
44	34759.0
45	42915.0
46	51071.0
47	51071.0
48	51323.0
49	51575.0
50	46535.5
51	41496.0
52	37546.0
53	33596.0
54	33596.0
55	28825.0
56	24054.0
57	19862.0
58	15670.0
59	13519.5
60	11369.0
61	11369.0
62	9833.5
63	8298.0
64	7057.5
65	5817.0
66	4860.0
67	3903.0
68	3903.0
69	3301.0
70	2699.0
71	2249.5
72	1800.0
73	1458.0
74	1116.0
75	1116.0
76	863.0
77	610.0
78	462.0
79	314.0
80	250.0
81	186.0
82	186.0
83	143.0
84	100.0
85	82.5
86	65.0
87	55.0
88	45.0
89	45.0
90	31.0
91	17.0
92	12.5
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	524557.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80971261827575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.4084402878331	47.384095226904115
2	9.958154315151045	11.31439769704347
3	2.743649692321075	4.67597851637943
4	1.1489473731442628	2.610854803273936
5	0.6293858439652524	1.787761446083847
6	0.3705014323388926	1.2628847937499217
7	0.29025671316844054	1.1542580322437084
8	0.2138111330424075	0.9717239218181677
9	0.158482886504868	0.8103030513530727
>10	0.9399686056262154	9.90057741951636
>50	0.07413163229292451	2.8925340883684494
>100	0.05474859059498699	6.3982320643683686
>500	0.0047607470082597384	1.835048501794539
>1k	0.0037405869350612226	3.4514134680557147
>5k	0.0010201600731985154	3.549936969046895
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6665	1.2705959504877449	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6263	1.1939598556496245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5448	1.0385906584031859	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2745	0.5232987072901515	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2370	0.4518098128516062	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1822	0.34734070844541204	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1617	0.3082601128190073	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1549	0.2952967932941511	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1444	0.27527990285135834	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1427	0.27203907297014435	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1375	0.262125946274666	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1220	0.23257720324006737	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1178	0.22457044706295026	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1119	0.21332286100461917	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	981	0.1870149478512345	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	926	0.1765299100002478	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	883	0.16833251677129465	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	769	0.14659989286197686	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	738	0.1406901442550571	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	736	0.14030887015138488	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	620	0.11819497213839487	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	608	0.11590732751636142	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	575	0.10961630480576944	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	567	0.10809120839108048	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	546	0.10408783030252193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.9063705183612077E-4	0.0
12	0.0	0.0	0.0	1.9063705183612077E-4	0.0
13	0.0	0.0	0.0	5.719111555083623E-4	0.0
14	0.0	0.0	0.0	0.0011438223110167246	0.0
15	0.0	0.0	0.0	0.0019063705183612076	0.0
16	0.0	0.0	0.0	0.0026689187257056906	0.0
17	0.0	0.0	0.0	0.00495656334773914	0.0
18	0.0	0.0	0.0	0.005528474503247502	0.0
19	0.0	0.0	0.0	0.006291022710591985	0.0
20	0.0	0.0	0.0	0.008006756177117071	0.0
21	0.0	0.0	0.0	0.011056949006495004	0.0
22	0.0	0.0	0.0	0.01696669761341475	0.0
23	0.0	0.0	0.0	0.03012065419010708	0.0
24	0.0	0.0	0.0	0.045562255388832865	0.0
25	0.0	0.0	0.0	0.05280646335860545	0.0
26	0.0	0.0	0.0	0.06443532352060882	0.0
27	0.0	0.0	0.0	0.14259651477341834	0.0
28	0.0	0.0	0.0	0.21789815024868603	0.0
29	0.0	0.0	0.0	0.29529679329415104	0.0
30	0.0	0.0	0.0	0.4607697542879039	0.0
31	0.0	0.0	0.0	0.6773334451737371	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1725	0.0	28.634785	1
ATGGGCG	35	8.8629423E-4	26.42857	19
ATCCACG	80	2.7186616E-8	23.125	1
ATAGTCG	40	0.0019297956	23.125	20
TAGGCGC	45	0.003823078	20.555555	13
TCTATAC	45	0.003823078	20.555555	3
GTATTGG	110	1.7480488E-9	20.181818	1
CGGCTCT	85	1.2426681E-6	19.588236	6
ATTGGAC	105	2.2524546E-8	19.38095	3
CGTCTGT	150	1.2732926E-11	18.5	34
ACGGCTC	90	2.1476808E-6	18.5	5
CCCGCAT	60	9.2276925E-4	18.5	13
CACGGCT	95	3.5988305E-6	17.526316	4
TAAGCAA	75	2.0645677E-4	17.266666	10
GTATCAA	2985	0.0	17.22948	2
ATAGCCT	65	0.0015783858	17.076925	3
TTGGACC	245	0.0	16.612244	4
GCGTCTG	170	8.367351E-11	16.32353	33
GAAGACT	80	3.3781826E-4	16.1875	4
GGACCCT	260	0.0	15.653848	6
>>END_MODULE