FastQCFastQC Report
Wed 25 May 2016
SRR2088287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913996
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162830.8507332303724772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155620.8130633501846399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136820.7148395294452026No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG63210.3302514738797782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60420.31567464090834046No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT37830.1976493158815379No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT37340.19508922693673342No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34560.1805646406784549No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA33120.1730411139835193No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA30070.15710586646994038No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT28000.1462907968459704No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT26810.14007343798001667No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG25170.13150497702189554No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21430.11196470630032665No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.1084641765186552No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20670.10799395610022174No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT19540.10209007751322363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC604.3259206E-827.75000235
GGTATCA44050.025.5346181
ACGCGCG1251.6592821E-716.2821
CCCGTAT803.3840365E-416.18751
GTATCAA72050.015.99653152
ACCGTCA700.002593339415.8571428
GTTGCGT700.002593339415.85714213
ACGGACC1302.591387E-715.6538468
CGGTATA957.063597E-515.57894826
TTAGACT1802.0190782E-1015.4166674
GAACCGT855.3664664E-415.2352946
TCTATAC2109.094947E-1214.976193
GTATTGG4950.014.9494951
TTGGACC6850.014.8540154
TAGACTA1252.961051E-614.8000015
GACGCGA750.004105733214.833
CGGTACT1001.0939677E-414.7999994
ATTGGAC5000.014.4300013
CGTCTGT4300.014.19767434
CTATGCG1203.3026135E-513.87500134
Produced by FastQC (version 0.11.5)