##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1913996
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.931892752126963	31.0	30.0	33.0	27.0	34.0
2	30.780871015404422	31.0	31.0	34.0	27.0	34.0
3	30.79071481863076	31.0	31.0	34.0	27.0	34.0
4	34.97024131711874	35.0	35.0	37.0	32.0	37.0
5	34.84394220259603	35.0	35.0	37.0	32.0	37.0
6	34.938039577930155	35.0	35.0	37.0	32.0	37.0
7	34.578978221480085	35.0	35.0	37.0	30.0	37.0
8	34.782127548855904	35.0	35.0	37.0	32.0	37.0
9	36.2398145032696	38.0	35.0	39.0	32.0	39.0
10	36.141437599660605	38.0	35.0	39.0	31.0	39.0
11	36.22428834752006	38.0	35.0	39.0	32.0	39.0
12	35.95738183360885	37.0	35.0	39.0	30.0	39.0
13	36.12208489463928	38.0	35.0	39.0	31.0	39.0
14	37.05611140253167	39.0	36.0	40.0	31.0	41.0
15	37.097072303181406	39.0	36.0	40.0	31.0	41.0
16	37.027716881330996	39.0	36.0	40.0	31.0	41.0
17	37.080625560346	39.0	36.0	40.0	31.0	41.0
18	37.06049542423286	39.0	36.0	40.0	31.0	41.0
19	37.13658179013958	39.0	36.0	40.0	31.0	41.0
20	37.09087479806645	39.0	36.0	40.0	31.0	41.0
21	37.02062491248675	39.0	36.0	40.0	31.0	41.0
22	36.94099778682923	39.0	36.0	40.0	31.0	41.0
23	36.822448949736575	39.0	35.0	40.0	31.0	41.0
24	36.74817867957927	39.0	35.0	40.0	30.0	41.0
25	36.65126207160308	38.0	35.0	40.0	30.0	41.0
26	36.407417779347504	38.0	35.0	40.0	30.0	41.0
27	36.26302406065634	38.0	35.0	40.0	30.0	41.0
28	36.113378502358415	38.0	34.0	40.0	29.0	41.0
29	35.9545035621809	38.0	34.0	40.0	29.0	41.0
30	35.81115112048301	38.0	34.0	40.0	29.0	41.0
31	35.60616636607391	38.0	34.0	40.0	27.0	41.0
32	35.343851815782266	38.0	34.0	40.0	27.0	41.0
33	35.538766538697054	38.0	34.0	40.0	27.0	41.0
34	35.553683497771154	38.0	34.0	40.0	27.0	41.0
35	35.502291018372034	38.0	34.0	40.0	27.0	41.0
36	35.43009285285863	38.0	34.0	40.0	27.0	41.0
37	35.28476757527184	38.0	34.0	40.0	26.0	41.0
38	35.184039047103546	38.0	34.0	40.0	26.0	41.0
39	35.19516655207221	38.0	34.0	40.0	26.0	41.0
40	35.09190771558561	38.0	33.0	40.0	25.0	41.0
41	34.96809293227363	38.0	33.0	40.0	25.0	41.0
42	34.83419819059183	38.0	33.0	40.0	24.0	41.0
43	34.49618285513658	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	5.0
16	19.0
17	63.0
18	191.0
19	365.0
20	750.0
21	1515.0
22	2893.0
23	4985.0
24	8020.0
25	12810.0
26	19201.0
27	27741.0
28	38055.0
29	51134.0
30	65525.0
31	81594.0
32	99989.0
33	120255.0
34	144816.0
35	173093.0
36	209141.0
37	258439.0
38	309458.0
39	283936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.628975191170724	24.4088284406028	12.961103366987183	23.00109300123929
2	18.483842181488363	23.11509532935283	34.899968442985255	23.501094046173556
3	19.829090551913378	26.34916687391196	31.02958417885931	22.79215839531535
4	13.79611033669872	16.51868655942855	35.69965663460112	33.98554646927162
5	12.26282604561347	39.486811884664334	34.638212410057285	13.61214965966491
6	32.079116152802825	36.752009931055234	15.637284508431575	15.531589407710362
7	25.372728051678266	33.900384326822	22.240589844492884	18.48629777700685
8	27.06928332138625	34.04583917625742	20.249676592845546	18.635200909510782
9	25.593000194357774	13.802588929130469	22.511645792363204	38.09276508414855
10	16.27762022491165	27.556065947891216	34.055557064905045	22.110756762292084
11	32.78543946800307	22.375438611156973	23.856423942369787	20.982697978470174
12	20.068693978461816	27.064058650070322	31.57848814731065	21.288759224157207
13	30.249018284259737	21.217808187686913	27.46066344966238	21.07251007839097
14	21.07083818356987	22.41232479064742	27.765940994652027	28.75089603113068
15	23.55532613443288	29.08036380431307	25.405643480968614	21.958666580285435
16	22.468176527014684	28.200738141563512	26.45831025770169	22.87277507372011
17	20.529405495100303	27.49039182945001	27.512387695690066	24.467814979759623
18	21.150932394843043	26.705019237239785	30.52890392665397	21.6151444412632
19	21.038340727984803	25.89979289402904	31.388414604837212	21.67345177314895
20	23.652348280769655	25.081922846233745	30.56093116182061	20.70479771117599
21	23.344562893548368	25.77293787447832	29.55408475252822	21.328414479445097
22	22.54879320541945	25.429520228882403	30.142121509135862	21.879565056562292
23	21.718436193179087	26.649010760733045	30.566208079849698	21.066344966238173
24	20.992154633552005	26.243367279764428	29.772371520107672	22.992106566575895
25	21.826116669000353	26.71891686294015	30.355758319244135	21.099208148815357
26	22.554070123448533	26.146345133427655	29.52811813608806	21.77146660703575
27	21.86253262807237	26.878269338075945	29.289455150376494	21.969742883475202
28	21.511330222215722	26.732553255074727	29.54326968290425	22.21284683980531
29	20.796699679623153	26.583023162012875	30.377336211778918	22.242940946585048
30	20.69027312491771	26.89420458558952	31.086585342916077	21.32893694657669
31	21.433273632755764	26.511027191279396	30.450847337194016	21.604851838770823
32	21.826430149279307	26.75282497978052	29.439821190848885	21.980923680091287
33	22.50527169335777	26.523148428732345	29.1948885995582	21.776691278351677
34	21.547798428000895	27.829943218272142	29.327960977974875	21.29429737575209
35	20.84555035642708	28.32346567077465	29.523311438477407	21.307672534320865
36	21.287714289894023	26.317505365737443	30.542279085222752	21.852501259145786
37	21.338707081937475	26.15972029199643	30.273051772313003	22.22852085375309
38	21.405635121494505	26.042530914380173	30.6048184008744	21.947015563250915
39	21.95333741554319	25.558308376820015	30.79849696655583	21.689857241080965
40	20.879615213406925	25.94566550818288	30.513282159419354	22.661437118990843
41	21.210336907705134	25.538088898827375	30.524097229043324	22.727476964424167
42	19.817700768444656	26.586784925360345	30.68104635537378	22.914467950821212
43	20.080344995496333	26.18150717138385	30.635330481359418	23.1028173517604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	284.0
2	344.0
3	826.0
4	1308.0
5	1308.0
6	2170.5
7	3033.0
8	3318.5
9	3604.0
10	5478.5
11	7353.0
12	7353.0
13	12516.0
14	17679.0
15	27511.5
16	37344.0
17	36432.5
18	35521.0
19	35521.0
20	38589.0
21	41657.0
22	35780.0
23	29903.0
24	33940.0
25	37977.0
26	37977.0
27	42830.0
28	47683.0
29	51615.5
30	55548.0
31	60422.5
32	65297.0
33	65297.0
34	71086.0
35	76875.0
36	83526.5
37	90178.0
38	95505.0
39	100832.0
40	100832.0
41	104845.0
42	108858.0
43	118741.0
44	128624.0
45	146610.0
46	164596.0
47	164596.0
48	166610.0
49	168624.0
50	156313.0
51	144002.0
52	135915.5
53	127829.0
54	127829.0
55	115355.5
56	102882.0
57	92092.0
58	81302.0
59	73714.0
60	66126.0
61	66126.0
62	58455.5
63	50785.0
64	44632.5
65	38480.0
66	33145.5
67	27811.0
68	27811.0
69	23850.0
70	19889.0
71	16758.5
72	13628.0
73	10873.5
74	8119.0
75	8119.0
76	6347.0
77	4575.0
78	3582.0
79	2589.0
80	1984.0
81	1379.0
82	1379.0
83	1054.0
84	729.0
85	542.0
86	355.0
87	297.0
88	239.0
89	239.0
90	197.0
91	155.0
92	97.5
93	40.0
94	29.5
95	19.0
96	19.0
97	9.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1913996.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.27170499782323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1132457725646	38.26342637905184
2	13.083880408852156	13.154979522812289
3	4.529576019499476	6.831285282524761
4	2.088535289144428	4.199769197336486
5	1.1155591449932198	2.804053012236153
6	0.6532543787232767	1.9704126849427719
7	0.42680404713555264	1.5019317004632882
8	0.30423389340321866	1.2235485231604646
9	0.2192926140140859	0.992179223992588
>10	1.2753803183896182	11.89354320607408
>50	0.12120396373832303	4.196471811273468
>100	0.06094427335345097	5.4964662930920225
>500	0.004937976374883648	1.762613124642783
>1k	0.0026265831781295997	2.6517366215934186
>5k	2.1012665425036798E-4	0.6529781271712432
>10k+	3.15189981375552E-4	2.4046052896323857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16283	0.8507332303724772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15562	0.8130633501846399	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13682	0.7148395294452026	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6321	0.3302514738797782	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6042	0.31567464090834046	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3783	0.1976493158815379	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3734	0.19508922693673342	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3456	0.1805646406784549	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3312	0.1730411139835193	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3007	0.15710586646994038	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2800	0.1462907968459704	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2681	0.14007343798001667	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2517	0.13150497702189554	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2143	0.11196470630032665	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2076	0.1084641765186552	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2067	0.10799395610022174	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1954	0.10209007751322363	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.224671315927515E-5	0.0
4	0.0	0.0	0.0	5.224671315927515E-5	0.0
5	5.224671315927515E-5	0.0	0.0	5.224671315927515E-5	0.0
6	2.089868526371006E-4	0.0	0.0	5.224671315927515E-5	0.0
7	2.089868526371006E-4	0.0	0.0	5.224671315927515E-5	0.0
8	2.089868526371006E-4	0.0	0.0	5.224671315927515E-5	0.0
9	2.089868526371006E-4	0.0	0.0	1.5674013947782544E-4	0.0
10	2.089868526371006E-4	0.0	0.0	3.6572699211492605E-4	0.0
11	2.089868526371006E-4	0.0	0.0	4.179737052742012E-4	0.0
12	2.089868526371006E-4	0.0	0.0	5.747138447520266E-4	0.0
13	2.089868526371006E-4	0.0	0.0	7.314539842298521E-4	0.0
14	2.089868526371006E-4	0.0	0.0	0.001044934263185503	0.0
15	2.089868526371006E-4	0.0	0.0	0.0016196481079375296	0.0
16	2.089868526371006E-4	0.0	0.0	0.0022466086658488316	0.0
17	2.089868526371006E-4	0.0	0.0	0.0033960363553528846	0.0
18	2.089868526371006E-4	0.0	0.0	0.0040229969132641865	0.0
19	2.089868526371006E-4	0.0	0.0	0.004754450897494039	0.0
20	2.089868526371006E-4	0.0	0.0	0.006112865439635192	0.0
21	2.089868526371006E-4	0.0	0.0	0.00830722739232475	0.0
22	2.089868526371006E-4	0.0	0.0	0.01389762570036719	0.0
23	2.089868526371006E-4	0.0	0.0	0.02476494203749642	0.0
24	2.089868526371006E-4	0.0	0.0	0.039446268435252736	0.0
25	2.089868526371006E-4	0.0	0.0	0.046238341145958504	0.0
26	2.089868526371006E-4	0.0	0.0	0.05637420349885789	0.0
27	2.089868526371006E-4	0.0	0.0	0.12283202263745588	0.0
28	2.089868526371006E-4	0.0	0.0	0.19127521687610632	0.0
29	2.089868526371006E-4	0.0	0.0	0.271526168288753	0.0
30	2.089868526371006E-4	0.0	0.0	0.4131147609503886	0.0
31	2.089868526371006E-4	0.0	0.0	0.6337526306220076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGC	60	4.3259206E-8	27.750002	35
GGTATCA	4405	0.0	25.534618	1
ACGCGCG	125	1.6592821E-7	16.28	21
CCCGTAT	80	3.3840365E-4	16.1875	1
GTATCAA	7205	0.0	15.9965315	2
ACCGTCA	70	0.0025933394	15.857142	8
GTTGCGT	70	0.0025933394	15.857142	13
ACGGACC	130	2.591387E-7	15.653846	8
CGGTATA	95	7.063597E-5	15.578948	26
TTAGACT	180	2.0190782E-10	15.416667	4
GAACCGT	85	5.3664664E-4	15.235294	6
TCTATAC	210	9.094947E-12	14.97619	3
GTATTGG	495	0.0	14.949495	1
TTGGACC	685	0.0	14.854015	4
TAGACTA	125	2.961051E-6	14.800001	5
GACGCGA	75	0.0041057332	14.8	33
CGGTACT	100	1.0939677E-4	14.799999	4
ATTGGAC	500	0.0	14.430001	3
CGTCTGT	430	0.0	14.197674	34
CTATGCG	120	3.3026135E-5	13.875001	34
>>END_MODULE