FastQCFastQC Report
Wed 25 May 2016
SRR2088286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2674692
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261000.9758132899040338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT237850.889261268213312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214170.8007277099568848No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG156340.5845158994007534No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91250.3411607766426938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91130.3407121268542322No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA87480.32706569578852446No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT81950.3063904180369179No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT76910.2875471269215296No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60680.22686724303209493No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG58850.22002533375805514No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT57110.21351991182536156No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT53030.19826581901766635No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48670.18196487670356062No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47340.17699234154811097No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45450.16992610737984037No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT35470.1326133999727819No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34090.12745392740547323No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33350.12468725370995987No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.11709759478848406No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC30690.11474218339906053No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28680.10722729944232831No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28290.10576918762982804No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG27960.10453540071155855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59500.029.444541
ATTGGAC10700.021.9579433
GTATTGG11250.021.2133331
CGTATCG656.9065085E-519.92307710
TTGGACC17150.018.877554
TATTGGA12750.018.427452
CGGACCA953.6100082E-617.5263169
GTATCAA103450.017.382312
TGGACCC18800.016.7287225
GGACCCT17950.016.6963776
CGTCTGT4900.016.61224634
CCGATAA957.065276E-515.5789489
ACCGTTA957.065276E-515.5789488
ACGAACG957.065276E-515.57894815
TATACCG1954.1836756E-1115.1794875
ACGTTTA1601.0983058E-815.0312526
TCCGACG1853.0559022E-1015.023
TCCGATA1001.09422566E-414.88
GACCCTC20050.014.4862857
GGCGTAA908.2790747E-414.38888913
Produced by FastQC (version 0.11.5)