##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2674692
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.035333040215473	31.0	30.0	33.0	27.0	34.0
2	30.817728172066168	31.0	31.0	34.0	27.0	34.0
3	30.802998251761323	31.0	31.0	34.0	27.0	34.0
4	34.96464265792099	35.0	35.0	37.0	32.0	37.0
5	34.84957669892459	35.0	35.0	37.0	32.0	37.0
6	34.94995348997193	35.0	35.0	37.0	32.0	37.0
7	34.57426612110852	35.0	35.0	37.0	30.0	37.0
8	34.79172443032693	35.0	35.0	37.0	32.0	37.0
9	36.22181170766578	38.0	35.0	39.0	32.0	39.0
10	36.136598905593615	38.0	35.0	39.0	31.0	39.0
11	36.22848051289644	38.0	35.0	39.0	32.0	39.0
12	35.96173391179246	38.0	35.0	39.0	30.0	39.0
13	36.129782793682416	38.0	35.0	39.0	31.0	39.0
14	37.0453353133744	39.0	36.0	40.0	31.0	41.0
15	37.0865011747147	39.0	36.0	40.0	31.0	41.0
16	37.00879017098043	38.0	36.0	40.0	31.0	41.0
17	37.07608464825109	39.0	36.0	40.0	31.0	41.0
18	37.06882437304931	39.0	36.0	40.0	31.0	41.0
19	37.14694551746519	39.0	36.0	40.0	31.0	41.0
20	37.10078767947861	39.0	36.0	40.0	31.0	41.0
21	37.020652845262184	39.0	36.0	40.0	31.0	41.0
22	36.94667124289451	39.0	36.0	40.0	31.0	41.0
23	36.81266478532855	39.0	35.0	40.0	31.0	41.0
24	36.74593523291654	39.0	35.0	40.0	30.0	41.0
25	36.655858319387804	38.0	35.0	40.0	30.0	41.0
26	36.40684908767066	38.0	35.0	40.0	30.0	41.0
27	36.261940440245084	38.0	35.0	40.0	30.0	41.0
28	36.11014501856663	38.0	35.0	40.0	29.0	41.0
29	35.9504290587477	38.0	34.0	40.0	29.0	41.0
30	35.7997175001832	38.0	34.0	40.0	29.0	41.0
31	35.573511267839436	38.0	34.0	40.0	27.0	41.0
32	35.348584061267616	38.0	34.0	40.0	27.0	41.0
33	35.52636453094412	38.0	34.0	40.0	27.0	41.0
34	35.528208107699875	38.0	34.0	40.0	27.0	41.0
35	35.45772859080597	38.0	34.0	40.0	27.0	41.0
36	35.39865749028299	38.0	34.0	40.0	27.0	41.0
37	35.255427540815916	38.0	34.0	40.0	26.0	41.0
38	35.15643408661633	38.0	34.0	40.0	26.0	41.0
39	35.15523843493008	38.0	34.0	40.0	26.0	41.0
40	35.03609275385727	38.0	33.0	40.0	25.0	41.0
41	34.90593197272808	38.0	33.0	40.0	25.0	41.0
42	34.73547271984961	38.0	33.0	40.0	24.0	41.0
43	34.413626316600194	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	1.0
15	16.0
16	35.0
17	87.0
18	190.0
19	519.0
20	1079.0
21	2141.0
22	3957.0
23	6900.0
24	11266.0
25	17595.0
26	26686.0
27	38580.0
28	53756.0
29	72654.0
30	92274.0
31	113437.0
32	139814.0
33	168907.0
34	201538.0
35	241381.0
36	293316.0
37	365097.0
38	437704.0
39	385758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.74117393703649	24.446702648379702	13.01962992374449	22.79249349083932
2	18.223070170322416	22.95359615237941	35.581928685620625	23.24140499167755
3	19.822282341293874	26.70711244509648	31.53409813167273	21.93650708193691
4	13.341573534448079	16.055680429746676	35.91045249322165	34.6922935425836
5	11.757615456284313	39.91726897900767	34.993561875535576	13.331553689172434
6	32.057859372219305	36.46834850517368	15.473033904464515	16.0007582181425
7	25.104871888052905	34.23627842009472	21.97942791169974	18.67942178015263
8	27.36120644919116	33.92801862793922	20.261884359021526	18.4488905638481
9	25.349535572693977	13.628634624098776	22.879381999871388	38.14244780333586
10	16.070934522554374	27.425325981458798	34.310941222391214	22.192798273595614
11	32.42878058482995	21.723809694723727	24.634350422403774	21.21305929804254
12	20.13644935566413	26.778709473838486	31.832749340858683	21.252091829638704
13	30.718901466037956	20.812415036946312	28.1562886493099	20.31239484770583
14	21.109047322084187	22.045678530462574	27.800509367059835	29.044764780393407
15	23.276437062659923	28.853041770790806	25.963026770932878	21.907494395616393
16	22.42119840340495	28.189937383444523	26.538756611976254	22.850107601174265
17	20.117381739654512	26.912407110800046	27.554948382841836	25.415262766703606
18	21.1688299063967	26.461701010807975	31.05322033340661	21.316248749388713
19	20.58109868351197	24.905858319387804	32.168339382627984	22.344703614472245
20	24.336596512794745	24.258344512190565	30.96670569919826	20.438353275816432
21	23.794328468474127	25.017871216573724	29.949953116097106	21.237847198855047
22	22.370500977308787	24.55804257088293	30.925018656353704	22.14643779545458
23	21.619386456459285	26.320862364713392	31.58318789602691	20.476563282800413
24	20.688064270577698	25.39855056208341	30.270476002470563	23.64290916486833
25	21.64338922014198	26.291849678392875	31.048285185733537	21.016475915731604
26	22.798699812913036	25.52940675038472	29.973582004956086	21.69831143174616
27	21.56820299309229	26.72490888670546	29.752135946867902	21.95475217333435
28	21.424298573443224	26.24642388731114	29.872673190034593	22.456604349211048
29	20.69954222766584	26.07253470679989	30.771842141076434	22.456080924457844
30	20.198549963883693	26.398142290775912	32.035015620490135	21.368292124850264
31	21.440749065686816	25.87079185192164	31.28935967206691	21.39909941032463
32	22.14931663159721	26.055934664626808	29.567217459056966	22.227531244719017
33	22.94006188376082	25.931060473504985	29.242245462281264	21.886632180452928
34	21.5134303314176	28.23573704934998	29.312982578928715	20.937850040303708
35	20.5784067847812	29.141374034842144	29.3876453812252	20.892573799151453
36	21.466882915864705	26.19314672493132	31.059052780656614	21.280917578547363
37	21.45338603472848	26.530793078231063	30.32737975064045	21.688441136400005
38	21.580578249757355	26.167573686989005	30.838092759839263	21.413755303414376
39	22.305671082876085	25.619510582900762	30.89866048128158	21.17615785294157
40	20.88633008959536	26.25764013202268	30.150499571539452	22.705530206842507
41	21.88382811927504	25.81385071626939	29.971787405802235	22.33053375865333
42	19.759845245732965	27.415680011006877	30.39617271820456	22.428302025055597
43	20.380963490375713	26.993650109993972	30.004651002807055	22.62073539682326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	230.0
2	319.0
3	889.0
4	1459.0
5	1459.0
6	2570.0
7	3681.0
8	4063.0
9	4445.0
10	6727.0
11	9009.0
12	9009.0
13	16410.5
14	23812.0
15	38549.0
16	53286.0
17	52709.5
18	52133.0
19	52133.0
20	56391.5
21	60650.0
22	51710.0
23	42770.0
24	47979.0
25	53188.0
26	53188.0
27	59789.5
28	66391.0
29	70534.5
30	74678.0
31	81379.0
32	88080.0
33	88080.0
34	96296.0
35	104512.0
36	113218.5
37	121925.0
38	127570.0
39	133215.0
40	133215.0
41	139736.0
42	146257.0
43	160947.0
44	175637.0
45	210060.0
46	244483.0
47	244483.0
48	249637.0
49	254791.0
50	235858.5
51	216926.0
52	206422.0
53	195918.0
54	195918.0
55	172831.5
56	149745.0
57	126836.0
58	103927.0
59	92875.5
60	81824.0
61	81824.0
62	72633.5
63	63443.0
64	55674.5
65	47906.0
66	41021.0
67	34136.0
68	34136.0
69	29238.5
70	24341.0
71	20836.0
72	17331.0
73	13957.5
74	10584.0
75	10584.0
76	8362.5
77	6141.0
78	4780.0
79	3419.0
80	2673.0
81	1927.0
82	1927.0
83	1480.5
84	1034.0
85	805.0
86	576.0
87	456.5
88	337.0
89	337.0
90	289.0
91	241.0
92	144.0
93	47.0
94	31.5
95	16.0
96	16.0
97	12.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2674692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.58677946147521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12969479118799	32.60617573739692
2	14.286309233456834	12.739610382211536
3	5.158852213441931	6.900498177452359
4	2.47296359856787	4.410459303424069
5	1.336983156015529	2.9805886560485746
6	0.7786724739141228	2.083109872027818
7	0.49033316877731775	1.5303663801244398
8	0.36282642954353084	1.2941809597481504
9	0.2594977211246772	1.0413150896287242
>10	1.5013588300291711	12.53521807599512
>50	0.1350606268936908	4.17560365460881
>100	0.07650595242242228	6.162795259967338
>500	0.004919571071729917	1.5426203453176877
>1k	0.004919571071729039	4.219722797005013
>5k	7.633817180269199E-4	2.4899382425927428
>10k+	3.392807635675199E-4	3.2877970664506253
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26100	0.9758132899040338	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23785	0.889261268213312	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21417	0.8007277099568848	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15634	0.5845158994007534	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9125	0.3411607766426938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9113	0.3407121268542322	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8748	0.32706569578852446	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8195	0.3063904180369179	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7691	0.2875471269215296	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6068	0.22686724303209493	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5885	0.22002533375805514	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5711	0.21351991182536156	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5303	0.19826581901766635	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4867	0.18196487670356062	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4734	0.17699234154811097	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4545	0.16992610737984037	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3547	0.1326133999727819	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3409	0.12745392740547323	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3335	0.12468725370995987	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3132	0.11709759478848406	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3069	0.11474218339906053	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2868	0.10722729944232831	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2829	0.10576918762982804	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2796	0.10453540071155855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.738748237180206E-5	0.0
4	0.0	0.0	0.0	3.738748237180206E-5	0.0
5	0.0	0.0	0.0	3.738748237180206E-5	0.0
6	0.0	0.0	0.0	3.738748237180206E-5	0.0
7	0.0	0.0	0.0	1.1216244711540618E-4	0.0
8	3.738748237180206E-5	0.0	0.0	1.1216244711540618E-4	0.0
9	2.990998589744165E-4	0.0	0.0	1.4954992948720824E-4	0.0
10	2.990998589744165E-4	0.0	0.0	2.990998589744165E-4	0.0
11	3.3648734134621855E-4	0.0	0.0	4.1126230608982267E-4	0.0
12	3.3648734134621855E-4	0.0	0.0	5.234247532052289E-4	0.0
13	3.738748237180206E-4	0.0	0.0	5.608122355770309E-4	0.0
14	4.1126230608982267E-4	0.0	0.0	6.729746826924371E-4	0.0
15	4.1126230608982267E-4	0.0	0.0	9.720745416668536E-4	0.0
16	4.1126230608982267E-4	0.0	0.0	0.001233786918269468	0.0
17	4.1126230608982267E-4	0.0	0.0	0.0019815365657055092	0.0
18	4.1126230608982267E-4	0.0	0.0	0.0022806364246799257	0.0
19	5.98199717948833E-4	0.0	0.0	0.002953611107372363	0.0
20	5.98199717948833E-4	0.0	0.0	0.003551810825321196	0.0
21	5.98199717948833E-4	0.0	0.0	0.0051968600496804865	0.0
22	5.98199717948833E-4	0.0	0.0	0.008449571016027266	0.0
23	5.98199717948833E-4	0.0	0.0	0.013908143442310367	0.0
24	5.98199717948833E-4	0.0	0.0	0.02232032697596583	0.0
25	5.98199717948833E-4	0.0	0.0	0.026358175072120453	0.0
26	5.98199717948833E-4	0.0	0.0	0.032003684910262564	0.0
27	5.98199717948833E-4	0.0	0.0	0.07223261594232158	0.0
28	6.35587200320635E-4	0.0	0.0	0.1158638078702146	0.0
29	6.35587200320635E-4	0.0	0.0	0.16820628319073747	0.0
30	6.729746826924371E-4	0.0	0.0	0.26174976408498624	0.0
31	6.729746826924371E-4	0.0	0.0	0.39402667671642194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5950	0.0	29.44454	1
ATTGGAC	1070	0.0	21.957943	3
GTATTGG	1125	0.0	21.213333	1
CGTATCG	65	6.9065085E-5	19.923077	10
TTGGACC	1715	0.0	18.87755	4
TATTGGA	1275	0.0	18.42745	2
CGGACCA	95	3.6100082E-6	17.526316	9
GTATCAA	10345	0.0	17.38231	2
TGGACCC	1880	0.0	16.728722	5
GGACCCT	1795	0.0	16.696377	6
CGTCTGT	490	0.0	16.612246	34
CCGATAA	95	7.065276E-5	15.578948	9
ACCGTTA	95	7.065276E-5	15.578948	8
ACGAACG	95	7.065276E-5	15.578948	15
TATACCG	195	4.1836756E-11	15.179487	5
ACGTTTA	160	1.0983058E-8	15.03125	26
TCCGACG	185	3.0559022E-10	15.0	23
TCCGATA	100	1.09422566E-4	14.8	8
GACCCTC	2005	0.0	14.486285	7
GGCGTAA	90	8.2790747E-4	14.388889	13
>>END_MODULE