FastQCFastQC Report
Wed 25 May 2016
SRR2088285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272895
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316321.3917052921494393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT283651.2479678999689823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253071.113425829173807No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG166040.7305220874699447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107630.4735370529654911No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98680.4341599589950262No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96830.4260205596826955No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA93470.4112376506613812No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT91920.4044181539402393No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA73300.32249619978045624No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT68830.3028296511717435No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG68330.30062981351976226No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58440.25711702476357245No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49090.21598006067152245No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48710.21430818405601668No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48080.21153638861452026No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40560.17845083032872175No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39840.17528306410986869No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.16780361609313232No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35430.15588049601939377No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34400.15134883045631234No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31800.13990967466600965No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30850.1357299831272452No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30350.13353014547526393No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29510.12983441821993535No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC27060.11905521372522708No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.11901121697218744No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24700.10867198000787542No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA24570.10810002221836028No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA24270.10678011962717152No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23800.10471227223430911No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC23090.10158850276849568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.030.0105341
ATTGGAC9300.021.6827953
GTATTGG9300.021.4838711
TTGGACC13150.020.399244
CAATGCG951.6779086E-719.47368419
TATTGGA10550.018.9383872
GGACCCT13650.018.7032976
TATCCGT500.00703694618.510
CGTCTGT6550.018.3587834
GTATCAA121600.018.180512
GAACCGT752.0683877E-417.2666666
TGGACCC15150.017.095715
CGATCTG2450.016.61224433
ACGTTTA1452.9849616E-916.58620826
GACCCTC15350.015.9087947
TGAACCG700.00259353915.8571435
TCACGTT1659.749783E-1015.6969724
CGTGTCT2055.456968E-1215.34146335
AGTATCA4850.015.2577321
TTTTTAC15300.014.99346351
Produced by FastQC (version 0.11.5)