##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2272895
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87984134770854	31.0	30.0	33.0	27.0	34.0
2	30.693748721344363	31.0	31.0	34.0	27.0	34.0
3	30.71241742359414	31.0	30.0	34.0	27.0	34.0
4	34.89160079986097	35.0	35.0	37.0	32.0	37.0
5	34.77938752120094	35.0	35.0	37.0	32.0	37.0
6	34.884690229861036	35.0	35.0	37.0	32.0	37.0
7	34.51376108443197	35.0	35.0	37.0	30.0	37.0
8	34.73776703279298	35.0	35.0	37.0	31.0	37.0
9	36.134560989398985	38.0	35.0	39.0	31.0	39.0
10	36.06602108764373	37.0	35.0	39.0	31.0	39.0
11	36.152149131394104	38.0	35.0	39.0	31.0	39.0
12	35.88532598294246	37.0	35.0	39.0	30.0	39.0
13	36.06197822600692	37.0	35.0	39.0	31.0	39.0
14	36.92677532398109	38.0	36.0	40.0	31.0	41.0
15	36.97134447477776	38.0	36.0	40.0	31.0	41.0
16	36.874572296564516	38.0	36.0	40.0	31.0	41.0
17	36.993478801264466	38.0	36.0	40.0	31.0	41.0
18	36.994273382624364	38.0	36.0	40.0	31.0	41.0
19	37.08992452357016	39.0	36.0	40.0	31.0	41.0
20	37.054641767437566	39.0	36.0	40.0	31.0	41.0
21	36.98279111001608	39.0	36.0	40.0	31.0	41.0
22	36.91233118995818	39.0	36.0	40.0	31.0	41.0
23	36.78869811407918	39.0	35.0	40.0	31.0	41.0
24	36.71564634529972	38.0	35.0	40.0	30.0	41.0
25	36.62653488172573	38.0	35.0	40.0	30.0	41.0
26	36.362662155532924	38.0	35.0	40.0	30.0	41.0
27	36.2019873333348	38.0	35.0	40.0	30.0	41.0
28	36.01658985566865	38.0	34.0	40.0	29.0	41.0
29	35.850601105638404	38.0	34.0	40.0	29.0	41.0
30	35.69022018175059	38.0	34.0	40.0	28.0	41.0
31	35.42115847850429	38.0	34.0	40.0	27.0	41.0
32	35.231806572674934	37.0	34.0	40.0	27.0	41.0
33	35.32859986932964	38.0	34.0	40.0	27.0	41.0
34	35.29540739893396	38.0	34.0	40.0	27.0	41.0
35	35.18108711577086	38.0	33.0	40.0	26.0	41.0
36	35.09427624241331	38.0	33.0	40.0	26.0	41.0
37	34.93137298467373	38.0	33.0	40.0	25.0	41.0
38	34.80112411704016	38.0	33.0	40.0	25.0	41.0
39	34.74592579067665	38.0	33.0	40.0	24.0	41.0
40	34.57036906676287	38.0	33.0	40.0	24.0	41.0
41	34.39223061338073	38.0	33.0	40.0	23.0	41.0
42	34.1951511178475	38.0	33.0	40.0	22.0	41.0
43	33.85128173540793	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	17.0
16	33.0
17	69.0
18	218.0
19	472.0
20	1065.0
21	2111.0
22	3819.0
23	6653.0
24	10687.0
25	16798.0
26	24880.0
27	35874.0
28	49826.0
29	65754.0
30	83465.0
31	101276.0
32	122793.0
33	146486.0
34	174346.0
35	206477.0
36	249316.0
37	308310.0
38	355723.0
39	306421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.24655560419641	25.50100202605048	13.857041350348345	21.395401019404765
2	18.11478312900508	23.138068410551302	36.14738032333214	22.599768137111482
3	20.21787192105223	26.643993673266912	32.18450478354698	20.95362962213389
4	13.293838914688097	16.090888492429258	35.10474526979909	35.51052732308356
5	11.076886525774396	40.870387765382915	34.602126363074404	13.450599345768282
6	31.695524870264574	35.892419139467506	15.162337019527957	17.24971897073996
7	24.030498549207067	35.73464678306741	21.44309349969972	18.7917611680258
8	27.72917358698928	33.840498571205444	20.1314622980824	18.298865543722872
9	25.04695553468154	13.61774301056582	24.0197633414654	37.31553811328724
10	16.54462700652692	27.96759199171101	33.55764344591369	21.93013755584838
11	31.11683557753438	21.67359248887432	25.194300660611248	22.015271272980055
12	19.980597431909526	27.553538548855094	31.872303823977788	20.59356019525759
13	31.24561407368136	20.912624648300955	29.140589424500472	18.70117185351721
14	20.844121703818256	22.595764432584875	28.590102050468673	27.970011813128192
15	23.100187206184184	28.77031275091898	27.23324218672662	20.896257856170212
16	21.653177995463935	28.328057389364663	27.43787108511392	22.580893530057484
17	19.04104677074832	26.906830275925635	28.2905721557749	25.76155079755114
18	20.31963641083288	26.227212431722542	32.84876776093924	20.604383396505337
19	19.537374141788337	24.40909060911305	34.331150361103354	21.722384887995265
20	24.430253047325106	23.40957237355883	32.98779750054446	19.1723770785716
21	23.68481606057473	24.29496303172826	31.47747696220019	20.542743945496824
22	21.78662014743312	23.98795368901775	32.59574243420836	21.629683729340773
23	20.773682902201816	26.276488795126916	33.743617721012185	19.20621058165907
24	19.40670378526065	25.027244989319787	32.07222506978985	23.493826155629716
25	20.512034211875164	26.227476412240776	33.22568794422971	20.034801431654344
26	22.21426858697828	25.014705914703494	31.66265929574397	21.10836620257425
27	20.797661132608415	26.689574309415963	31.28921485594363	21.223549702031992
28	20.53574846176352	26.139482906161525	31.37852826461407	21.94624036746088
29	19.47912244076387	25.923854819514318	32.63630744051089	21.960715299210918
30	18.894493586373326	26.19205902604388	34.44681782484453	20.46662956273827
31	20.343042683449962	25.51952465908016	33.28261974266299	20.854812914806885
32	21.531878947333688	25.948712984981707	30.953871604275605	21.565536463409
33	22.621942500643453	25.86393124187435	30.493225599950723	21.020900657531474
34	20.709447642763966	28.87845676989038	30.57941523915535	19.832680348190305
35	19.278189269631902	29.882374680748562	30.75566623183209	20.08376981778745
36	20.365129053475854	25.74514000866736	33.231583509137025	20.65814742871976
37	20.411149657155303	26.348995444136225	32.14921938760919	21.090635511099283
38	20.722074710886336	25.783681164330076	32.721529151148644	20.772714973634944
39	21.72902839770425	25.256512069409276	32.69081941752698	20.32364011535949
40	19.98605302928644	26.143134636663813	31.459966254490418	22.41084607955933
41	21.023760446479052	25.777345631892366	31.385391758088254	21.81350216354033
42	18.413081114613743	27.612362207669072	31.723462808444737	22.25109386927245
43	19.179020588280586	27.390354591831123	31.28208738195121	22.14853743793708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	253.0
2	338.0
3	915.0
4	1492.0
5	1492.0
6	2757.5
7	4023.0
8	4562.5
9	5102.0
10	7823.5
11	10545.0
12	10545.0
13	19348.0
14	28151.0
15	45638.5
16	63126.0
17	61029.5
18	58933.0
19	58933.0
20	63502.0
21	68071.0
22	54319.0
23	40567.0
24	44002.0
25	47437.0
26	47437.0
27	51929.5
28	56422.0
29	58716.5
30	61011.0
31	64375.0
32	67739.0
33	67739.0
34	73007.5
35	78276.0
36	85460.5
37	92645.0
38	97404.5
39	102164.0
40	102164.0
41	107638.5
42	113113.0
43	130937.5
44	148762.0
45	190143.5
46	231525.0
47	231525.0
48	235628.5
49	239732.0
50	216682.0
51	193632.0
52	180999.0
53	168366.0
54	168366.0
55	144618.5
56	120871.0
57	99196.5
58	77522.0
59	67227.5
60	56933.0
61	56933.0
62	50015.0
63	43097.0
64	37483.0
65	31869.0
66	27062.0
67	22255.0
68	22255.0
69	18816.0
70	15377.0
71	12743.0
72	10109.0
73	8116.5
74	6124.0
75	6124.0
76	4731.5
77	3339.0
78	2599.5
79	1860.0
80	1439.0
81	1018.0
82	1018.0
83	786.0
84	554.0
85	441.5
86	329.0
87	257.5
88	186.0
89	186.0
90	135.0
91	84.0
92	51.0
93	18.0
94	13.0
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2272895.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.528347308222905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4220739886528	30.906257359990352
2	13.534300439910806	11.241142584849001
3	4.884452254272619	6.085296888775969
4	2.2865693309864064	3.7982978128613754
5	1.26010748776675	2.616509069883492
6	0.7705256460721146	1.919919398398537
7	0.4939143834403233	1.4358013639225569
8	0.3435085457299498	1.141227375233274
9	0.2560431552965882	0.9569744171144972
>10	1.484288401585618	11.797176157939345
>50	0.1538129581552988	4.461492046547846
>100	0.09440314175065388	7.429243156544088
>500	0.006979981977920644	2.0225589491196536
>1k	0.007624288006651781	6.265767574791811
>5k	8.590747049748485E-4	2.897779259024454
>10k+	5.369216906092804E-4	5.02455658500376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31632	1.3917052921494393	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28365	1.2479678999689823	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25307	1.113425829173807	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16604	0.7305220874699447	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10763	0.4735370529654911	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9868	0.4341599589950262	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9683	0.4260205596826955	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9347	0.4112376506613812	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9192	0.4044181539402393	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7330	0.32249619978045624	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6883	0.3028296511717435	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6833	0.30062981351976226	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5844	0.25711702476357245	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4909	0.21598006067152245	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4871	0.21430818405601668	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4808	0.21153638861452026	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4056	0.17845083032872175	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3984	0.17528306410986869	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3814	0.16780361609313232	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3543	0.15588049601939377	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3440	0.15134883045631234	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3180	0.13990967466600965	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3085	0.1357299831272452	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3035	0.13353014547526393	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2951	0.12983441821993535	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2706	0.11905521372522708	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2705	0.11901121697218744	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2470	0.10867198000787542	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2457	0.10810002221836028	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2427	0.10678011962717152	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2380	0.10471227223430911	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2309	0.10158850276849568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.3996753039625675E-5	0.0
10	0.0	0.0	0.0	4.3996753039625675E-5	0.0
11	0.0	0.0	0.0	1.3199025911887703E-4	0.0
12	0.0	0.0	0.0	5.279610364755081E-4	0.0
13	0.0	0.0	0.0	6.159545425547594E-4	0.0
14	0.0	0.0	0.0	7.919415547132622E-4	0.0
15	0.0	0.0	0.0	0.0012319090851095188	0.0
16	0.0	0.0	0.0	0.002155840898941658	0.0
17	0.0	0.0	0.0	0.0034757434901304283	0.0
18	0.0	0.0	0.0	0.004047701279645562	0.0
19	0.0	0.0	0.0	0.004663655822200322	0.0
20	0.0	0.0	0.0	0.005719577895151338	0.0
21	0.0	0.0	0.0	0.007567441522815617	0.0
22	0.0	0.0	0.0	0.012891048640610322	0.0
23	0.0	0.0	0.0	0.022218360285010966	0.0
24	0.0	0.0	0.0	0.036649295282008186	0.0
25	0.0	0.0	0.0	0.04192890564676327	0.0
26	0.0	0.0	0.0	0.050508272489490275	0.0
27	0.0	0.0	0.0	0.10471227223430911	0.0
28	0.0	0.0	0.0	0.16916751543736072	0.0
29	0.0	0.0	0.0	0.24523790144287352	0.0
30	0.0	0.0	0.0	0.39302299490297615	0.0
31	0.0	0.0	0.0	0.6033714711854266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7120	0.0	30.010534	1
ATTGGAC	930	0.0	21.682795	3
GTATTGG	930	0.0	21.483871	1
TTGGACC	1315	0.0	20.39924	4
CAATGCG	95	1.6779086E-7	19.473684	19
TATTGGA	1055	0.0	18.938387	2
GGACCCT	1365	0.0	18.703297	6
TATCCGT	50	0.007036946	18.5	10
CGTCTGT	655	0.0	18.35878	34
GTATCAA	12160	0.0	18.18051	2
GAACCGT	75	2.0683877E-4	17.266666	6
TGGACCC	1515	0.0	17.09571	5
CGATCTG	245	0.0	16.612244	33
ACGTTTA	145	2.9849616E-9	16.586208	26
GACCCTC	1535	0.0	15.908794	7
TGAACCG	70	0.002593539	15.857143	5
TCACGTT	165	9.749783E-10	15.69697	24
CGTGTCT	205	5.456968E-12	15.341463	35
AGTATCA	485	0.0	15.257732	1
TTTTTAC	1530	0.0	14.9934635	1
>>END_MODULE