FastQCFastQC Report
Wed 25 May 2016
SRR2088284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909532
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184352.026866564342981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141991.5611325384923234No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG110491.214800578759186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92461.0165667618071712No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT63480.6979413588526846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.6229577409041134No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG46600.5123514070972764No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT45250.49750860882299913No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA35340.3885514748244152No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30860.3392953738845912No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29590.3253321488413822No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23480.2581547433185419No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT22350.24573077142970232No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT20880.22956861330882256No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.22352154734522808No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17540.19284643091172163No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC15600.17151678005831572No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15320.1684382737495767No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.1652498207869542No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA14820.16294094105539994No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14570.16019227470831152No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA13860.1523860622825805No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC13280.1460091563573354No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.14216102347141166No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12600.138532783893255No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12490.1373233707005361No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11590.12742817185101787No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG11300.12423971888839536No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC11290.12412977223451181No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG11230.12347009231121062No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10970.12061147931023868No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT10390.1142345733849936No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT10280.11302516019227472No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC10100.11104612042237107No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9640.10598857434372842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.031.0339491
TGCGTCG303.598893E-430.83333425
CCGTATA454.007048E-628.7777792
CCCGTAT451.3229155E-424.6666681
CGTATAC551.9020927E-523.5454543
AGTATCA1450.021.6896551
GTATCAA52500.021.4600012
GGCGTAG555.1419594E-420.1818181
AGTACGT656.900105E-519.9230777
GTGGTAT4850.018.6907221
CGGTATA500.0070337818.521
GTTATAC609.2347944E-418.53
GGACCCT4900.017.74496
TTGGACC4750.017.5263164
GCCCCGA752.0666685E-417.26666611
CCCCGAA752.0666685E-417.26666612
GTAAGAT752.0666685E-417.2666663
AGGCCGT650.001579591117.0769236
CGAATTA650.001579591117.07692315
GCTAACG650.001579591117.07692326
Produced by FastQC (version 0.11.5)