##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909532
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.502014222699145	31.0	30.0	31.0	26.0	34.0
2	30.1518231354147	31.0	30.0	31.0	26.0	34.0
3	30.092658642026887	31.0	30.0	31.0	26.0	34.0
4	34.17401696696763	35.0	33.0	37.0	30.0	37.0
5	33.927965151308584	35.0	33.0	37.0	30.0	37.0
6	34.21615512153503	35.0	35.0	37.0	30.0	37.0
7	33.875139082517165	35.0	33.0	37.0	29.0	37.0
8	34.14978912231785	35.0	33.0	37.0	30.0	37.0
9	35.39835431848467	37.0	34.0	39.0	30.0	39.0
10	35.22949384958418	37.0	34.0	39.0	29.0	39.0
11	35.4656559637264	37.0	34.0	39.0	30.0	39.0
12	35.1201969804251	37.0	34.0	39.0	29.0	39.0
13	35.3425289049753	37.0	34.0	39.0	30.0	39.0
14	36.03627909738195	38.0	34.0	40.0	29.0	41.0
15	36.146834855728	38.0	34.0	40.0	29.0	41.0
16	35.93501273182252	38.0	34.0	40.0	29.0	41.0
17	36.09446946341635	38.0	34.0	40.0	29.0	41.0
18	36.09542819823822	38.0	34.0	40.0	29.0	41.0
19	36.224326356851655	38.0	34.0	40.0	29.0	41.0
20	36.191803037166366	38.0	34.0	40.0	29.0	41.0
21	36.13084311492064	38.0	34.0	40.0	29.0	41.0
22	36.03696736343526	38.0	34.0	40.0	29.0	41.0
23	35.850253756877166	38.0	34.0	40.0	28.0	41.0
24	35.77084038824363	38.0	34.0	40.0	27.0	41.0
25	35.65041801717806	38.0	34.0	40.0	27.0	41.0
26	35.36616633609373	38.0	34.0	40.0	27.0	41.0
27	35.11763302445653	37.0	33.0	40.0	27.0	41.0
28	34.91054850186689	37.0	33.0	40.0	26.0	41.0
29	34.68947326757058	37.0	33.0	40.0	25.0	41.0
30	34.4957164783647	37.0	33.0	39.0	25.0	40.0
31	34.19062111063712	36.0	33.0	39.0	25.0	40.0
32	33.983888417339905	36.0	32.0	39.0	24.0	40.0
33	34.10742447764345	36.0	33.0	39.0	24.0	40.0
34	34.036127370999594	36.0	33.0	39.0	24.0	40.0
35	33.90497090811538	36.0	32.0	39.0	24.0	40.0
36	33.77192226331784	36.0	32.0	39.0	23.0	40.0
37	33.58130665001342	36.0	32.0	39.0	22.0	40.0
38	33.4231582836008	36.0	31.0	39.0	21.0	40.0
39	33.32161155407396	36.0	31.0	39.0	21.0	40.0
40	33.13483637738969	36.0	31.0	39.0	19.0	40.0
41	32.92645778268384	36.0	31.0	39.0	18.0	40.0
42	32.67153657045601	36.0	31.0	39.0	16.0	40.0
43	32.263166111802555	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	5.0
15	15.0
16	45.0
17	122.0
18	215.0
19	508.0
20	1021.0
21	1833.0
22	2983.0
23	4873.0
24	7696.0
25	11054.0
26	15440.0
27	20807.0
28	27387.0
29	34517.0
30	42538.0
31	50615.0
32	59037.0
33	69290.0
34	79583.0
35	92785.0
36	109677.0
37	122646.0
38	109550.0
39	45285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.73845889974184	27.000479367410936	14.138260116191622	19.1228016166556
2	22.737847596346253	25.245510878121934	30.817717243593407	21.198924281938403
3	25.518178579753105	26.092210059679044	30.589577936785073	17.80003342378278
4	18.0289423571683	18.43123716372816	31.353047501352343	32.1867729777512
5	11.053157008219612	43.56735112123597	32.969263313440315	12.410228557104093
6	32.56641877361105	35.44207350593492	14.396414859510164	17.59509286094387
7	23.58641587101938	36.71217725159753	21.125589863798087	18.57581701358501
8	29.176763434381638	34.58723827199043	19.538290021681483	16.69770827194645
9	25.333907987844295	13.231530061614105	24.09810759819336	37.33645435234824
10	17.528355242036564	29.281212755570994	32.101674267645336	21.0887577347471
11	31.801409955889405	20.989365959636388	24.564831143928966	22.644392940545245
12	18.129433598817855	28.857588298157733	32.598303303237266	20.414674799787143
13	31.939502953167125	20.130242806190438	30.259847921788346	17.67040631885409
14	20.554966730142535	22.52806938073647	29.518037848036133	27.398926041084863
15	24.499192991560495	28.260138180954602	27.661478650558745	19.579190176926158
16	21.298755843664654	28.20384549416623	28.69761591675719	21.799782745411928
17	18.321070616536854	27.279414028313465	29.718470598065817	24.681044757083864
18	19.291129943751294	25.92047338631296	33.66346648606096	21.124930183874785
19	18.995703284766233	23.86315159884424	35.99675437477735	21.14439074161217
20	23.799492486245676	23.216555327355167	34.90278516863618	18.08116701776298
21	22.413175127428172	23.85226688010977	33.45830602991428	20.27625196254777
22	21.018721716223286	22.867364754621057	35.451089131553374	20.662824397602282
23	20.09088190410013	25.99853551057027	35.95937251245695	17.95121007287264
24	19.03616365339537	24.191892093956014	33.82618753380859	22.94575671884002
25	19.828109401318482	26.48274057427336	34.68992844671766	18.999221577690502
26	21.957995980350333	24.101186104502094	33.25743349326907	20.683384421878504
27	21.203322148093743	26.240747989075704	32.93155161115827	19.62437825167229
28	20.280319988741464	25.816243958431368	33.42136395420942	20.482072098617753
29	19.057383357594894	24.68038507716056	35.51584771069077	20.74638385455377
30	18.663004710114652	25.19746419037483	36.56814713501009	19.571383964500424
31	19.785120259650018	25.089056789645664	34.56634840775256	20.55947454295176
32	21.51095288565988	25.979404792794536	32.532115417599385	19.977526903946206
33	22.94696613203274	25.703218798239096	31.43165935887907	19.918155710849096
34	20.836430164084383	28.586020063065398	31.901021624307884	18.676528148542324
35	19.47298170927466	28.95829943311505	32.12883109115457	19.439887766455715
36	20.449417942414343	24.99879058680728	35.318053680354296	19.233737790424087
37	20.72868244327852	25.798982333771654	33.79727156383723	19.6750636591126
38	20.991564892714052	25.571172866924968	33.94789847965767	19.489363760703306
39	22.028911572105216	25.165579660748605	33.5763887361852	19.229120030960978
40	20.4376536504488	25.945211383436757	31.704546953818006	21.912588012296432
41	21.655092948901196	26.248664148155314	31.434188131918393	20.662054771025097
42	19.002739870614775	28.538303215279946	30.73569703979629	21.723259874308987
43	20.80388595453486	27.16265068188915	30.63762462453218	21.395838739043814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	77.0
2	80.0
3	192.0
4	304.0
5	304.0
6	779.0
7	1254.0
8	1480.5
9	1707.0
10	2909.5
11	4112.0
12	4112.0
13	8632.5
14	13153.0
15	21628.5
16	30104.0
17	27159.0
18	24214.0
19	24214.0
20	29271.5
21	34329.0
22	26130.0
23	17931.0
24	18789.5
25	19648.0
26	19648.0
27	21054.5
28	22461.0
29	23378.0
30	24295.0
31	25577.5
32	26860.0
33	26860.0
34	29203.5
35	31547.0
36	34151.5
37	36756.0
38	38267.5
39	39779.0
40	39779.0
41	42488.5
42	45198.0
43	52499.0
44	59800.0
45	78136.5
46	96473.0
47	96473.0
48	94788.0
49	93103.0
50	83257.0
51	73411.0
52	68439.0
53	63467.0
54	63467.0
55	54465.5
56	45464.0
57	37880.5
58	30297.0
59	26056.5
60	21816.0
61	21816.0
62	19235.0
63	16654.0
64	14303.0
65	11952.0
66	10117.5
67	8283.0
68	8283.0
69	7020.0
70	5757.0
71	4781.0
72	3805.0
73	3060.5
74	2316.0
75	2316.0
76	1842.0
77	1368.0
78	1080.5
79	793.0
80	619.5
81	446.0
82	446.0
83	337.5
84	229.0
85	178.5
86	128.0
87	112.5
88	97.0
89	97.0
90	75.5
91	54.0
92	31.5
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	909532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.569218574059285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45130434475406	39.56026203862585
2	10.346343540432938	10.050276417151876
3	3.2218200148582232	4.6944384152378404
4	1.4386465163249473	2.794957484087813
5	0.7702412944753887	1.8705008893070763
6	0.4960398478840082	1.4455360675992908
7	0.3352758902759712	1.1398861598198176
8	0.26637011556807055	1.034991069169844
9	0.20443704171156943	0.8936412627170962
>10	1.2828978407207525	11.910975404648061
>50	0.11224834951982926	3.730939746157722
>100	0.05977393653640387	5.668833869481126
>500	0.006844343877152063	2.2479648292663783
>1k	0.006388054285341924	5.760583012721013
>5k	6.844343877152062E-4	2.3557811539509634
>10k+	6.844343877152062E-4	4.840432180058323
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18435	2.026866564342981	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14199	1.5611325384923234	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11049	1.214800578759186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9246	1.0165667618071712	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6348	0.6979413588526846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5666	0.6229577409041134	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4660	0.5123514070972764	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4525	0.49750860882299913	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3534	0.3885514748244152	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3086	0.3392953738845912	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2959	0.3253321488413822	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2348	0.2581547433185419	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2235	0.24573077142970232	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2088	0.22956861330882256	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.22352154734522808	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1754	0.19284643091172163	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1560	0.17151678005831572	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1532	0.1684382737495767	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1503	0.1652498207869542	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1482	0.16294094105539994	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1457	0.16019227470831152	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1386	0.1523860622825805	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1328	0.1460091563573354	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1293	0.14216102347141166	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1260	0.138532783893255	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1249	0.1373233707005361	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1159	0.12742817185101787	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1130	0.12423971888839536	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1129	0.12412977223451181	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1123	0.12347009231121062	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1097	0.12061147931023868	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1039	0.1142345733849936	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1028	0.11302516019227472	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1010	0.11104612042237107	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	964	0.10598857434372842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.099466538835357E-4	0.0
3	0.0	0.0	0.0	1.099466538835357E-4	0.0
4	0.0	0.0	0.0	1.099466538835357E-4	0.0
5	0.0	0.0	0.0	1.099466538835357E-4	0.0
6	1.099466538835357E-4	0.0	0.0	1.099466538835357E-4	0.0
7	1.099466538835357E-4	0.0	0.0	1.099466538835357E-4	0.0
8	1.099466538835357E-4	0.0	0.0	1.099466538835357E-4	0.0
9	1.099466538835357E-4	0.0	0.0	1.099466538835357E-4	0.0
10	1.099466538835357E-4	0.0	0.0	2.198933077670714E-4	0.0
11	1.099466538835357E-4	0.0	0.0	3.2983996165060714E-4	0.0
12	1.099466538835357E-4	0.0	0.0	3.2983996165060714E-4	0.0
13	1.099466538835357E-4	0.0	0.0	3.2983996165060714E-4	0.0
14	1.099466538835357E-4	0.0	0.0	5.497332694176785E-4	0.0
15	1.099466538835357E-4	0.0	0.0	7.6962657718475E-4	0.0
16	1.099466538835357E-4	0.0	0.0	0.001099466538835357	0.0
17	1.099466538835357E-4	0.0	0.0	0.0017591464621365713	0.0
18	1.099466538835357E-4	0.0	0.0	0.0017591464621365713	0.0
19	1.099466538835357E-4	0.0	0.0	0.001869093116020107	0.0
20	1.099466538835357E-4	0.0	0.0	0.0023088797315542497	0.0
21	1.099466538835357E-4	0.0	0.0	0.0027486663470883926	0.0
22	1.099466538835357E-4	0.0	0.0	0.004287919501457893	0.0
23	1.099466538835357E-4	0.0	0.0	0.0067067458868956785	0.0
24	1.099466538835357E-4	0.0	0.0	0.011984185273305392	0.0
25	1.099466538835357E-4	0.0	0.0	0.014512958312626714	0.0
26	2.198933077670714E-4	0.0	0.0	0.01715167800583157	0.0
27	2.198933077670714E-4	0.0	0.0	0.03518292924273143	0.0
28	2.198933077670714E-4	0.0	0.0	0.05662252675002089	0.0
29	2.198933077670714E-4	0.0	0.0	0.08531860341362371	0.0
30	2.198933077670714E-4	0.0	0.0	0.14611910301121897	0.0
31	2.198933077670714E-4	0.0	0.0	0.2130766152262922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3535	0.0	31.033949	1
TGCGTCG	30	3.598893E-4	30.833334	25
CCGTATA	45	4.007048E-6	28.777779	2
CCCGTAT	45	1.3229155E-4	24.666668	1
CGTATAC	55	1.9020927E-5	23.545454	3
AGTATCA	145	0.0	21.689655	1
GTATCAA	5250	0.0	21.460001	2
GGCGTAG	55	5.1419594E-4	20.181818	1
AGTACGT	65	6.900105E-5	19.923077	7
GTGGTAT	485	0.0	18.690722	1
CGGTATA	50	0.00703378	18.5	21
GTTATAC	60	9.2347944E-4	18.5	3
GGACCCT	490	0.0	17.7449	6
TTGGACC	475	0.0	17.526316	4
GCCCCGA	75	2.0666685E-4	17.266666	11
CCCCGAA	75	2.0666685E-4	17.266666	12
GTAAGAT	75	2.0666685E-4	17.266666	3
AGGCCGT	65	0.0015795911	17.076923	6
CGAATTA	65	0.0015795911	17.076923	15
GCTAACG	65	0.0015795911	17.076923	26
>>END_MODULE